乙酸奥曲肽中有机溶剂残留量的毛细管GC测定

建立了毛细管气相色谱法测定乙酸奥曲肽中有机溶剂乙醚、甲醇、二氯甲烷、N,N-二甲基甲酰胺的含量.用HP-Innowax毛细管柱,程序升温的方法.各组分的线性范围分别为25～300、25～300、15～180和25～300μg/ml(r均大于0.999).平均回收率分别为99.7%、99.5%、99.3%和99.2%.检测限分别为1、1、0.6和1ng.     

兰索拉唑中有机溶剂残留量的毛细管GC测定

建立了毛细管气相色谱法测定兰索拉唑中甲醇、丙酮、乙腈、氯仿的残留量.采用RTX-50毛细管柱,程序升温.各溶剂检测限分别为18、50.4、1.46和0.4ng,平均回收率分别为102.3%、100.4%、101.8%和1013%.     

顶空气相色谱法检测百乐眠胶囊中树脂残留物

建立气相色谱法检测百乐眠胶囊中树脂残留物的方法.采用气相色谱法对百乐眠胶囊中苯、甲苯、二甲苯、正己烷、苯乙烯、1,4-二乙苯的残留量进行检测,气相色谱条件为:色谱柱HP-5,载气为高纯氮气,柱前压为10Psi,气化室温度200℃,柱温60℃(5min),以30℃*min-1的速率升至90℃(3.5min),再以30℃*min-1的速率升至140℃(4min),最后以30℃*min-1的速率升至190℃(3min),检测器为FID,温度为220℃.正己烷在1.815～29.060μg*ml-1,苯在0.0128～0.1966μg*ml-1内,甲苯在0.685～11.125μg*ml-1内,二甲苯在1.698～27.170μg*ml-1内,苯乙烯在1.695～27.125μg*ml-1内,1,4-二乙苯在1.699～27.140μg*ml-1内均有良好的线性关系,相关系数分别为0.9996、0.9991、0.9996、0.9998、0.9966、0.9980.本法简便、快速、准确度高,适于测定百乐眠胶囊中树脂残留物,可作为百乐眠胶囊的质量监控方法.     

顶空气相色谱法测定盐酸依匹斯汀中溶剂残留

建立顶空气相色谱法分析盐酸依匹斯汀中溶剂残留.采用DB-1大口径毛细管柱,以正丙醇为内标进行定量.甲醇、乙醚、三氯甲烷、四氢呋喃的线性范围分别为7.9 ～ 317.0μg·ml-1 (r=0.9996),0.7～35.5μg·ml-1(r=0.9998),1.5～73.5μg·ml-1 (r=0.9992),0.9～44.4μg·ml-1(r=0.9994),回收率分别为101.64%,97.18%,99.32%,101.12%,RSD分别为1.97%,1.10%,2.40%,0.84%.本方法简单、准确、灵敏度高、重现性好.     

Therapy for minimal residual tumor disease: β‐Galactosylceramide inhibits the growth of recurrent HPV16‐associated neoplasms after surgery and chemotherapy

Natural killer T (NKT) cells are potent modulators of antitumor immunity. Their protective effects can be achieved upon their activation by glycolipid ligands presented in the context of the CD1d molecule. These CD1d‐binding glycolipid antigens have been described as potent therapeutic agents against tumors, infections, as well as autoimmune diseases. Immunoregulatory and therapeutic effects of glycolipid ligands depend on their structure and modes of administration. Therefore, more studies are needed for optimization of the particular therapeutic settings. This study was focused on the tumor‐inhibitory effects of 12 carbon acyl chain β‐galactosyl ceramide (C12 β‐D ‐Galactosyl Ceramide; β‐GalCer(C12)) on the growth of human papillomavirus type 16 (HPV16)‐associated neoplasms transplanted in syngeneic mice. Treatment of tumor‐bearing mice with β‐GalCer(C12) 3–14 days after tumor cell transplantation significantly inhibited the growth of the major histocompatability complex (MHC) Class I‐positive (TC‐1), as well as MHC Class I‐deficient (TC‐1/A9) HPV16‐asssociated tumors. Moreover, administration of β‐GalCer(C12) after surgical removal of TC‐1 tumors inhibited the growth of tumor recurrences. Similar results were obtained in the treatment of tumors after chemotherapy. β‐GalCer(C12) treatment turned out to be also synergistic with immunotherapy based on administration of IL‐12‐producing cellular vaccines. These results suggest that β‐GalCer(C12), whose antitumor effects have so far not been studied in detail, can be effective for the treatment of minimal residual tumor disease as well as an adjuvant for cancer immunotherapy.     

急性髓系白血病的诊断及治疗:现状与挑战

近年来,对急性髓系白血病(AML)生物学特性认识的深入促进了分类和危险分层的更新,微小残留病检测丰富了AML的预后体系,单倍型相合移植等手段为AML患者提供了更多选择.     

谨慎选择异基因造血干细胞移植治疗骨髓增殖性疾病:适应证探讨及疗效回顾

简要回顾了真性红细胞增多症、原发性血小板增多症和原发性骨髓纤维化等骨髓增殖性疾病的临床特点,并建议以此作为其接受异基因造血干细胞移植的适应证及选择治疗方式的依据;介绍了迄今国内外骨髓增殖性疾病的移植治疗现状以及用JAK2突变,尤其是V617F检测移植后微小残留病变的意义.     

牙冠延长术后桩核修复在牙体缺损至龈下残根残冠患者中的应用观察

目的 观察牙冠延长术后桩核修复在牙体缺损至龈下残根残冠患者中的应用效果.方法 选取我院2016年10月～2019年11月期间接收的牙体缺损至龈下残根残冠患者140例,根据随机数字表法分成研究组(70例)与对照组(70例),其中对照组接受直接桩核冠修复治疗,研究组接受牙冠延长术后桩核修复治疗.比较两组临床疗效、美观满意度...     

探索Morphogo在多发性骨髓瘤MRD检测中的应用

目的：探索人工智能骨髓细胞识别系统Morphogo应用于多发性骨髓瘤微小残留病（minimal residual disease,MRD）检测的临床价值及面临问题。方法：收集已经由流式细胞术（multiparameter flow cytometry,MFC）检查后明确微小残留病结果的病例65例,调取其留存的骨髓瑞氏染色涂片,通过基于人工智能（artificial intelligence,AI）平台的分析系统Morphogo对所有骨髓涂片进行全自动扫描及细胞分类。AI及细胞形态学多发性骨髓瘤MRD阳性阈值设为浆细胞比例大于4.4%。按AI自动识别细胞数量将病例分为I 500组、I 1000组、I 2000组,每组病例的人工智能微小残留病（AI-MRD）、细胞形态学（morphology）微小残留病（M-MRD）和流式细胞术微小残留病（MFC-MRD）结果两两行Kappa一致性检验,并计算各组敏感度、特异度、准确度。分别以MFC-MRD和M-MRD结果为金标准绘制AI-MRD的受试者工作特征（receiver operating characteristic,ROC）曲线并计算其曲线下...     

残余胆囊结石6例诊治分析

目的 探讨残余胆囊结石的原因、诊治及预防.方法 回顾性分析绍兴市人民医院2016年10月至2019年12月收治的6例残余胆囊结石患者的临床诊治经过,分析其发生原因,探讨诊治方法及预防措施.结果 6例残余胆囊结石患者中,2例行腹部B超检查,其中1例证实为残余胆囊结石;所有患者行腹部增强CT检查,均证实为残余胆囊结石;3例...