参松养心胶囊治疗室性早搏的效果

目的：观察参松养心胶囊不同治疗方法对室性早搏治疗的疗效。方法：98例室性早搏的入选病例均使用参松养心胶囊并美托洛尔联合治疗,治疗一周后,随机均分为两组：一组持续使用参松养心胶囊联合美托洛尔治疗3个月（持续治疗组）,另一组使用参松养心胶囊联合美托洛尔治疗1个月后停用参松养心胶囊1周,再继续原方案治疗（间断治疗组）,观察两组室性早搏发作的情况。结果：治疗1月时,两组效果无显著差异（P〉0.05）,停用参松养心胶囊1周内,间断治疗组早搏发生率（65.1%,28/43）明显高于持续治疗组（2.4%,1/41）（x2=36.47,P〈0.05）,两组在3个月后抗心律失常作用方面没有显著差别（P〉0.05）。结论：参松养心胶囊在治疗室性早搏过程中,需要持续给药,如果短期中断治疗可出现病情反弹,再加用参松养心胶囊治疗仍然有效,对长期室性早搏的控制无显著影响。     

胆石症手术患者CIC、Ig、T淋巴细胞亚群和胆石中细菌感染及IgG型IC变化

目的研究胆石症手术患者CIC(循环免疫复合物)、Ig(免疫球蛋白)、T淋巴细胞亚群和胆石中IgG型IC(免疫复合物)及细菌的变化。方法胆石症患者50例(男20例,女30例),平均年龄46岁。术前、术后5d测定CIC、Ig、T淋巴细胞亚群,并培养结石细菌。结果与对照组比较,胆结石患者术前血中CIC(u/L,5.3±1.5vs1.2±0.8,P＜0.01)增高,IgG(g/L,8.7±2.0vs11.2±3.2,P＜0.05),CD3+(%,52±5vs66±10,P＜0.01),CD4+(%,29±4vs44±9,P＜0.01)和CD8+(%,20±3vs31±7,P＜0.01)则降低,但术后恢复正常。胆石中IC阳性率为82%,细菌培养阳性率为85%,主要是大肠杆菌。结论胆石症术前、术后血CIC、IgG、T淋巴细胞亚群均有明显的改变,结石中IC阳性率高,培养主要为大肠杆菌。且细菌培养阳性率高。     

钐、钇配合物对人胃癌细胞的作用及其机制研究

 目的 为了深入研究稀土配合物的抗肿瘤活性,将稀土配合物开发成抗癌新药,本实验对钐、钇配合物抑制胃癌细胞 BGC-823 细胞增殖的活性 作用及其机制作了探讨 。 方法 磺酰罗丹明 B （ SRB ）法测定钇、钐配合物及硝酸盐对人胃癌细胞株 BGC-83 增殖的影响;观察稀土配合物诱导 BGC-823 细胞凋亡,采用流式细胞仪检测稀土配合物对 BGC-823 细胞周期的影响,运用彗星微凝胶单细胞电泳观察其对 BGC-823 细胞 DNA 剪切作用。 结果 钇、钐配合物明显抑制 BGC-823 的增殖, 并能诱导 BGC-823 凋亡;经不同浓度钐、钇配合物处理 BGC-823 细胞的细胞周期发生明显变化并 损伤细胞的 DNA 。 结论 稀土配合物的体外抗肿瘤活性可能是在 5-Fu 配体的细胞毒性基础上,稀土离子发挥了协同作用; 钐、钇稀土配合物可能通过将胃癌 BGC-823 细胞阻滞于 G₀/G₁ 期或直接切断肿瘤细胞 DNA 诱导细胞凋亡,从而发挥其抗肿瘤活性作用。     

Dipicolinate Complexes of Oxovanadium(IV) and Dioxovanadium(V) with 2-Phenylpyridine and 4,4′-Dimethoxy-2,2′-bipyridyl as New Precatalysts for Olefin Oligomerization

Polyolefins are used in everyday life, including in the production of many types of plastic. In addition, polyolefins account for over 50% of the polymers produced in the world. After conducting the oligomerization reactions of 2-propen-1-ol, 2-chloro-2-propen-1-ol, and norborene, polyolefins are obtained. In this report, two complexes of oxovanadium(IV) and dioxovanadium(V) with dipicolinate, 2-phenylyridine, and 4,4′-dimethoxy-2,2′-bipyridyl as precatalysts for 2-propen-1-ol, 2-chloro-2-propen-1-ol, and norborene oligomerizations are prepared. We present for the first time the new dipicolinate complex compound of oxovanadium(IV) with 4,4′-dimetoxy-2,2′-bipyridyl. Both complexes were tested for catalytic activity in the oligomerization reactions of 2-propen-1-ol, 2-chloro-2-propen-1-ol, and norbornene. Both synthesized complexes showed high catalytic activity in these oligomerization reactions, except for the oligomerization of norbornene.     

突出腰椎间盘组织中免疫复合物的表达及意义

目的：检测突出腰椎间盘组织中是否有免疫复合物（Immune Complexes IC)的表达，以便进一步探讨突出腰椎间盘组织的致痛机制。方法：取76例不同类型突出腰椎间盘组织标本用生物素化羊抗人IgG抗体进行免疫组化染色。结果：48例脱出和游离型腰椎间盘组织标本中40例IC表达阳性（占83％），28例膨出和突出型中仅8例（占28％）表达阳性。结论：突出腰椎间盘组织中存在着IC的表达，且IC很可能是盘源性下腰痛及坐骨神经痛的重要病生基础之一。     

平板运动试验中室性早搏ST段下移诊断冠状动脉病变的价值

探讨平板运动试验中室性早搏(VPCs)ST段下移程度对反映冠状动脉(简称冠脉)病变的价值。将休息和平板运动试验中均发生VPCs并且在3个月内进行冠脉造影的92例患者按冠脉造影结果分成冠心病组和非冠心病组,比较两组VPCsST段下移程度,下移程度与冠脉狭窄程度的关系以及VPCsST段下移诊断冠心病的价值。结果:冠心病组运动中VPCsST段下移及ST/R均大于非冠心病组。其中ST/R>10%对冠心病诊断灵敏度为91%,特异度为75%;对单支、2支、3支血管病变诊断灵敏度分别为84%、91%、100%。冠脉狭窄≥90%组运动中VPCsST段下移和ST/R大于狭窄为50%~69%组。结论:运动试验中VPCsST段下移可作为诊断冠心病的有效参考指标,其下移程度可能与冠脉狭窄程度有关。     

急性心肌梗死患者组织因子活性和凝血酶-抗凝血酶复合物的变化及临床意义

目的探讨急性心肌梗死(AMI)患者血浆组织因子(TF)活性及凝血酶-抗凝血酶(TAT)复合物血浆水平及临床意义.方法采用发色底物法和酶联免疫吸附测定检测了AMI30例、不稳定型心绞痛(UA)27例,胆囊炎及健康人各30例的TF活性及TAT.结果 AMI组[TF活性(258±111) pmol/L和TAT(28.4±3.6) μg/L]显著高于对照组[TF活性(110±50) pmol/L和TAT(6.6±3.4) μg/L]、UA组[TF活性(121±49) pmol/L和TAT(9.8±5.6) μg/L]、胆囊炎组[TF活性(143±33) pmol/L和TAT(5.9±2.1) μg/L](P均<0.01).结论监测血浆TF活性和TAT的变化,有助于判断AMI和UA患者是否存在由高浓度TF触发的凝血途径激活及有无凝血酶生成引发的血液高凝状态.     

Palladium(II) Complexes with N‐Heteroaromatic Bidentate Hydrazone Ligands: The Effect of the Chelate Ring Size and Lipophilicity on in vitro Cytotoxic Activity

Novel Pd(II) complex with N‐heteroaromatic Schiff base ligand, derived from 8‐quinolinecarboxaldehyde (q8a) and ethyl hydrazinoacetate (haOEt), was synthesized and characterized by analytical and spectroscopy methods. The structure of novel complex, as well as structures of its quinoline and pyridine analogues, was optimized by density functional theory calculations, and theoretical data show good agreement with experimental results. A cytotoxic action of the complexes was evaluated on cultures of human promyelocytic leukemia (HL‐60), human glioma (U251), rat glioma (C6), and mouse fibrosarcoma (L929) cell lines. Among investigated compounds, only complexes with quinoline‐based ligands reduce the cell numbers in a dose‐dependent manner in investigated cell lines. The observed cytotoxic effect of two isomeric quinoline‐based complexes is predominantly mediated through the induction of apoptotic cell death in HL‐60 cell line. The cytotoxicity of most efficient novel Pd(II) complex is comparable to the activity of cisplatin, in all cell lines investigated.     

射频消融治疗起源于左室流出道的室性心动过速和频发室性期前收缩

目的 探讨源自左室流出道部位的室性心动过速(室速)和室性期前收缩(室早)的心电图特点和射频消融的安全性.方法 对9例于左室流出道部位消融的室速/室早病例的心电图和射频消融情况进行归纳总结.结果 消融成功部位6例在左冠窦内,3例在主动脉瓣下.心电图特点:(1)Ⅱ、Ⅲ、avF导联均呈高R波;(2)aVR和aVL导联均呈QS型,且aVL振幅多大于aVR;(3)Ⅰ导联多呈QS型;(4)V1导联R波偏高:R/S>0.62;(5)胸前导联R波移行不规则;(6)V5、V6多呈R型.9例均消融成功,无复发病例,无左主干及主动脉瓣损伤.结论 源自左室流出道的室速/室早具有独特的心电图表现,射频消融能安全有效地根治此类心律失常.     

Comparative fluorescence spectroscopy of root caries lesions

In this study, the light-emission properties of carious and sound root surfaces were investigated under a wide range of excitation wavelengths. Human molar teeth with exposed root surfaces containing light- and dark-discolored root caries (n = 3 of each) were selected. Emission spectra were recorded from carious and corresponding sound root surface areas from each tooth by using a fluorescence spectrophotometer at excitation wavelengths from 360 nm up to 580 nm, in steps of 20 nm. The spectra were corrected for fluctuations in detector sensitivity and excitation light intensity, and normalized to peak intensity. Excitation spectra were recorded for selected emission wavelengths that showed maximum intensity. Light- and dark-discolored root surface caries showed distinct fluorescence emission bands between 600 and 700 nm that were not present in sound root surface areas. These bands were strongest for excitation wavelengths between 390 and 420 nm. The excitation spectra of root caries revealed maximum excitation at around 405 nm, which is equivalent to the Soret band of porphyrin compounds. The emission spectra of both types of root caries lesions were shifted towards longer wavelengths (red shift at half maximum) when compared to the spectra of corresponding sound root surfaces. The red shift for dark-discolored root caries was higher than for light-discolored lesions at all excitation wavelengths.