P16、Cyclin D1蛋白表达与胰腺癌生物学行为

目的 探讨P16、CyclinD1蛋白表达水平与胰腺癌发生、发展及预后的关系。方法 应用免疫组织化学SP法结合微波抗原修复法对53例原发性胰腺癌和42例胰腺良性病变及正常组织中P16蛋白及CyclinD1蛋白进行检测。结果 胰腺癌组织、胰腺良性病变和正常组织中P16蛋白的阳性率分别为66．04％和87．71％（P＜0．05）；CyclinD1表达的阳性率分别为52．83％和23．81％（P＜0．05），P16及CyclinD1蛋白表达与胰腺癌的组织分化、病理分期及术后生存期密切相关。结论 P16及CyclinD1蛋白在胰腺癌中的表达可判断胰腺癌的预后。     

毛喉萜对人肝癌细胞增殖影响的实验研究

目的　探讨毛喉萜 (Forskolin)的抗肝癌作用。方法　采用克隆形成、MTT比色法观察毛喉萜对SMMC 772 1细胞的作用 ,并利用免疫细胞化学方法检测其对rasp2 1、p5 3蛋白表达的影响。结果　毛喉萜可明显抑制SMMC 772 1细胞的增殖 ,其抑制率与剂量呈正相关 (P <0 .0 1 ) ,经毛喉萜处理后 ,rasp2 1、p5 3蛋白表达均有明显下降。结论　毛喉萜能明显抑制肝癌SMMC 772 1细胞增殖 ,其作用可能与降低rasp2 1、p5 3蛋白表达有关。     

电离辐射调控脂质体介导的p16基因在HeLa细胞中的表达

目的探讨不同剂量γ射线照射后诱导脂质体介导的p16基因在HeLa细胞中的表达及抗癌作用。方法用脂质体Lipofectamin介导重组质粒Egr-p16转染人HeLa细胞，采用RT-PCR的方法检测了。Coγ射线照射转染后的人HeLa细胞剂量效应和时程变化，用细胞计数检测细胞增殖的变化，用流式细胞术检测细胞周期的变化。结果研究证实0．5～8Gv照射后p16的转录水平高于对照，在2～4Gy照射后达到峰值，2Gy照射后2—24h高于对照组，在照射后4h达到峰值，然后逐渐下降。细胞生长曲线显示体外稳定转染联合。Coγ射线照射对HeLa细胞的增殖具有明显的抑制作用。细胞周期变化显示转染后的HeLa细胞经过照射后G0／G1期比例呈现剂量依赖性的下降，而S期则出现剂量依赖性的增加，出现明显的S期阻滞，G2／M期在2Gy出现明显的阻滞，在5和10Gy时逐渐下降，但是仍然高于对照组。结论^60Coγ射线可诱导转染的HeLa细胞p16转录水平的增强，同时在细胞增殖上出现明显的变化。     

外源性p21WAF1/CIP1基因转染对人胶质瘤细胞生长和细胞周期的影响

目的：探讨外源性p21^WAF1／CIP1基因对胶质瘤细胞生长和细胞周期的影响．方法：采用脂质体介导法将p21^WAF1／CIP1基因质粒转导人人胶质瘤细胞系SHG44细胞，然后用电镜、流式细胞仪等技术对细胞形态、生长及细胞周期进行观察。结果：酶切电泳和斑点杂交显示p21^WAF1／CIP1基因成功的转染至SHG44细胞。转染p21^WAF1／CIP1基因的细胞光镜及电镜下可见凋亡产生；生长速度明显慢于亲代细胞，其倍增时间约是对照细胞的二倍；细胞周期发生明显改变，G1期明显增多，S期明显减少．结论：外源性p21^WAF1／CIP1基因对SHG44细胞的生长和细胞周期有明显的抑制作用，并可诱导细胞凋亡产生。     

Expression of p27 and p53: comparative analysis of uterine carcinosarcoma and endometrial carcinoma

The aim of the study was to assess both p27 and p53 expression in the stromal and epithelial component of carcinosarcoma and to assess if their expression in the latter is different than in endometrial carcinoma. Immunohistochemical staining for p27 and p53 was performed on paraffin-embedded tissue blocks of 18 uterine specimens with carcinosarcoma and their expression assessed. Their expression in the epithelial element was also compared to that in 35 paraffin-embedded tissue blocks of endometrial endometrioid carcinoma. Reduced p27 expression was observed in a similarly high proportion of the stromal (77.8%) as well as of the epithelial component (66.7%) of carcinosarcoma. Although statistically not significant, the proportion of reduced p27 expression in endometrial carcinoma (85.7%) was higher than in the epithelial element of carcinosarcoma. The percentage of p53 overexpression in both elements of carcinosarcomas and in endometrial carcinomas was low and also similar (27.8 and 20.0%, respectively). Our results indicate that reduced p27 expression is common and p53 overexpression is infrequent in carcinosarcoma. Their similar rates of expression in the stromal and epithelial elements of the tumor support the contention of a monoclonal origin of carcinosarcoma. Unexpectedly, reduced p27 expression is more common in endometrial carcinoma than in the epithelial element of carcinosarcoma, in spite of the less favorable prognosticators and outcome in the latter. Further studies of p27 expression in carcinosarcoma are indicated to establish its clinical value in this aggressive malignancy.     

CD44v6、P27蛋白在乳腺癌组织中的表达及意义

目的 探讨乳腺癌中CD44v6、p27蛋白表达的意义。方法 应用免疫组化S-P法检测65例乳腺癌中CD44v6、p27蛋白表达。结果 65例乳腺癌中CD44v6、p27蛋白表达总阳性率分别为58.4％、38.4％。CD44v6蛋白阳性表达率与乳腺癌组织学类型、腋淋巴结转移呈正相关(P<0.05,P<0.01),与预后呈负相关(P<0.01),与组织学分级无明显相关性(P>0.05);p27蛋白阳性表达与乳腺癌组织学类型、组织学分级及腋淋巴结转移呈负相关(P<0.05),与预后呈正相关(P<0.05)。结论 CD44v6、p27蛋白表达与乳腺癌浸润、转移及预后显著相关,检测乳腺癌中CD44v6、p27蛋白表达水平,对判断乳腺癌恶性程度、复发转移潜能,评估患者预后及术后选择合理治疗方案有一定参考价值。     

PCNA AND P53 EXPRESSION IN ORAL LEUKOPLAKIA WITH DIFFERENT DEGREES OF KERATINIZATION

Leukoplakias are oral lesions that may have many clinical and histological aspects and they are usually associated with malignancy when dysplastic alterations are shown. However, these transformations may occur in non-dysplastic lesions that show harmless clinical aspect. For this reason, the proposal was to study the p53 and PCNA immunohistochemical expression in non-dysplastic leukoplakias, trying to correlate the results only with the epithelial keratinization degree. For this, 24 leukoplakias degrees I, II and III of Grinspan were used, all of them located in oral mucosa. Most of the leukoplakias showed p53 and PCNA expression in their different keratinization degrees. The p53 marking was confined to the basal and parabasal layers, while the PCNA marking occurred in practically all epithelial layers. The expression pattern of these markers was histologically and statistically similar between the lesions with these keratinization variations. It was evident that non-dysplastic epithelium of leukoplakias showed submicroscopical signs of alterations that lead to malignant transformation, and that the keratinization degree did not correlate to a greater risk of this event.     

单亲大学生心理健康状况及心理问题的教育对策研究

目的针对单亲大学生心理健康问题提供有效的教育对策．方法针对大学新生开展16PF调查并发现单亲大学生的心理问题。结果本次测查共测量被试8302人，删除部分无效量表．实际有效数据为7742人，问卷有效率为93．27％．以一般情况问卷中“是否是单亲家庭子女”回答“是”的．作为单亲大学生的认定标准，检出单亲家庭子女555人，占总人数的7．17%，其中男生占40．0％．女生60．0％．17．5％的单亲出现各种心理问题．调查表明．单亲家庭子女与非单亲家庭子女相比，在乐群性和怀疑性两个因子有显着区别。事实上也表现为与正常大学生心理健康状况的显着差异。结论单亲大学生心理问题表现为：自卑心理、怀旧心理、逆反心理、猜疑心理等等。教育对策：加强社会教育系统、学校教育系统、家庭教育对单亲大学生心理的干预与影响，同时．单亲大学生遗要加强心理健康的自我教育．以多元系统帮助他们形成健全人格。     

p53、nm23蛋白在宫颈鳞癌中的表达及其临床意义

目的研究p53、nm23基因在宫颈鳞癌中的表达及其临床意义.方法采用免疫组织化学S-P法检测45例宫颈鳞癌组织、10例宫颈上皮内瘤变和10例正常宫颈鳞状上皮中p53、nm23蛋白的表达.结果p53、nm23蛋白在宫颈鳞癌中的阳性表达率分别为64.44%、46.67%;在CIN中阳性表达率分别为0、30.00%;在NE组均无表达.p53蛋白在宫颈鳞癌中阳性表达与在CIN、NE中的不表达差异均有显著性(P＜0.01).nm23蛋白在宫颈鳞癌组织中的阳性表达与在NE中的不表达差异有显著性(P＜0.05),但与在CIN中的表达差异无显著性(P＞0.05);与病理学分级、淋巴结转移均呈负相关(P＜0.05,P＜0.01).结论p53、nm23基因参与了宫颈鳞癌的发生发展过程.p53蛋白的过表达是宫颈癌组织恶性变的生物学标志;检测nm23蛋白对判断宫颈癌淋巴结转移和预后有重要意义.