Timing of IUI Treatment after hCG Administration 1-48 h Affecting Pregnancy Rate

Objective To compare the different time of administration of hCG affecting pregnancy rate of IUI.
Methods A total of 189 infertile couples underwent 331 cycles of lUI with husband＇s sperm. They were separated into 3 groups according to the time of hCG administration in IUI： hCG 1-23 h （group A）; hCG 24-36 h （group B）; hCG 37-48 h （group C）.
Results There were no statistical differences among 3 groups. None of the other relative factors, such as the female age, the different methods of ovulation and the cause of infertility, showed differences in pregnancy rate among 3 groups.
Conclusion IUI can be performed any time after administration of hCG （1-48 h）.     

电针/经皮穴位电刺激技术在生殖医学中的应用专家共识

不孕不育症近年来呈上升趋势,尽管现代医学解决了多种生殖障碍,但是总体活产率徘徊在20%~30%,仍有进一步提高的社会需求。针灸是中国传统医学的代表,在妇科不孕症治疗中积累了很多临床经验。本文从针刺/电针(EA)/经皮穴位电刺激(TEAS)相关的原理与技术演变入手,阐述其在生殖领域多种病症类型中的应用原则。从最佳刺激参数筛选,穴位辩证,疗程与治疗次数确定,疗效情况,基本原理等几方面详细论述EA/TEAS技术在诱导排卵、取卵镇痛、卵巢储备功能减退、改善子宫内膜容受性或胚胎种植及男性少弱精子症中的具体应用,以期为广大临床医生正确使用该技术提供帮助。     

少弱精夫妇接受传统体外受精和卵浆内单精子注射治疗结果的比较

本文将１２６个周期的ＩＶＦ和ＩＣＳＩ按男性精情况和治疗方法分３组,男方精液正常夫妇接受ＩＶＦ治疗共５１个周期为第１组,男方少、弱精夫妇接受ＩＶＦ治疗共２２个周期为第２组,男方少、弱精夫妇接受ＩＣＳＩ治疗共３０个周期为第３组。分别比较第１组与第２组、第３组与第２组的受精率、卵裂率和怀孕率。结果第２组的受精率明显低于第１组和第３组（Ｐ〈０．０１）,而不受精率为３１．８％,明显高于其它两组（Ｐ〈０．０１     

应用SELDI-TOF-MS技术分析少精子症患者精浆中的差异表达蛋白

目的:用表面增强激光解吸离子化飞行时间质谱(SELDI-TOF-MS)分析少精子症患者和正常对照组精浆蛋白质指纹图谱的变化,建立能鉴别少精子症患者和正常对照组精浆标志物的诊断模型。方法:收集33例少精子症和31例正常对照的精浆,用CM10(弱阳离子交换芯片)蛋白质芯片和SELDI-TOF-MS检测蛋白质指纹图谱的表达。用Biomarker Patterns Software分析软件进行数据处理,建立区分少精子症患者与正常对照组精浆中蛋白质指纹图谱差异表达模型,并用此模型对33例少精子症患者及31例正常对照组进行盲法交叉验证。结果:在相对分子质量2000~20000范围内,共检测到185种有差异的蛋白峰,其中23种有统计学意义(P<0.05)。建立了由3种差异蛋白质组成的少精子症诊断模型,其敏感性为90.9%(30/33),特异性为93.5%(29/31),双盲法验证结果其敏感性为87.9%(29/33),特异性为90.3%(28/31)。结论:用SELDI-TOF-MS技术初步建立的区分少精子症与正常对照精浆蛋白质差异表达模型可以区别少精子症和正常对照。     

五子衍宗软胶囊治疗肾虚精亏证男性少精、弱精不育症研究分析

目的：对采用五子衍宗软胶囊对患有由于肾虚精亏证所引起的男性少精和弱精不育症的患者进行治疗的效果进行研究分析。方法采用随机抽样的方法,在2006年5月～2010年5月这四年时间里,抽取150例在我院就诊的患有由于肾虚精亏证所引起的男性少精和弱精不育症的临床确诊患者病例,再将其随机分为A、B两组,平均每组75例。A组患者采用口服五子衍宗软胶囊的方法进行治疗;B组患者采用口服五子衍宗蜜丸进行治疗。对两组患者的临床治疗效果、用药后的并发症和不良反应现象进行比较分析。结果分析结果表明,A组患者的临床治疗效果与B组患者进行比较,其有效率和治愈率都明显高出很多,有显著的统计学差异（P〈0．05）;该组患者治疗前后的精子增加幅度明显比B组高出很多,有显著的统计学差异（P〈0．05）;两组患者在治疗的过程中均没有出现比较严重的并发症和不良反应现象,没有显著的统计学差异（P〉0．05）。结论采用五子衍宗软胶囊对患有由于肾虚精亏证所引起的男性少精和弱精不育症的患者进行治疗的效果十分明显,在治疗的过程中不会出现比较严重的并发症和不良反应现象,可以作为对该类患者采用中医方法进行治疗的首选方法,值得进一步的使用和推广。     

Geographical differences in semen quality in a population of young healthy volunteers from the different regions of Spain

Numerous studies have shown geographical differences in semen quality even within a given country. We have previously reported a low semen quality in volunteers from the province of Barcelona compared with the general population. The objective of this study was to determine the semen quality in a population of young healthy volunteers from the different regions of Spain. A total of 1239 volunteers between 18 and 30 years of age were enrolled. The parameters evaluated were semen volume, sperm concentration and progressive sperm motility. The results indicate that while there were no differences in semen volume or sperm motility, there were statistically significant differences in the rate of oligospermia in volunteers from the different regions studied. The prevalence of oligospermia was highest in Valencia (22.7%), Barcelona (22.7%) and Pais Vasco (18.7%), which are the regions of Spain with the highest degree of industrialisation for the last 50 years, and lowest in Galicia (8.5%) and Andalucía (13.7%), regions with a more recent industrial development. There were no differences in the rate of oligospermia as a function of age. These results support the hypothesis that exposure to environmental toxicants may affect the process of spermatogenesis leading to meiotic alterations, maturational arrest and oligospermia.     

445对不孕夫妇精液质量情况及相关社会因素分析

目的 通过分析445对不孕夫妇精液质量及其相关社会因素,探讨影响不孕的危险因素.方法 2008年1月～7月在上海瑞金医院生殖医学中心就诊的不育孕夫妇445对,先分析原/继发、不孕年限等基本情况以及精液质量各指标之间的相关情况,再根据男方精液质量情况分为3组,即精液正常组(83例)、少弱精子症组(344例)和无精子症组(18例).采用病例.对照研究方法,回顾性分析3组患者男方年龄、体重指数、原/继发、不孕年限、吸烟及精索静脉曲张等因素,采用R×C表卡方检验进行统计比较.结果 (1)原/继发不孕患者男性年龄及不孕年限存在差别;(2)精液质量各指标之间存在一定的相关关系,精子密度与A级、B级、C级、D级精子百分率明显相关:(3)与断发不孕相比,原发不孕与精液质量关系更为密切,精索静脉曲张也是影响精液质量的重要因素,吸烟、体重指数与不孕年限对精液质量影响不大.结论 应提倡适龄婚育,降低不孕风险,如出现不孕情况,尽早到不孕专科检查治疗.     

Y染色体上热休克转录因子在无、少精子症患者精液、睾丸中的缺失研究

目的 通过检测无、少精子症不育患者睾丸、精液基因组HSFY基因缺失情况,探讨其病因的基因诊断方法.方法 选择35例特发性无、少精子症患者作为研究对象,其中少精子症18例、严重少精子症12例、无精子症5例;10例正常已生育健康男性作为正常对照.应用PCR技术,检测每例患者睾丸、精液中精子基因组中的Y染色体上特异性序列标签位点(STS)的引物扩增了解基因缺失情况.结果 35例患者中5例表现睾丸、精液中精子基因组微缺失,其中少精子症2例,严重少精子症3例;其余30例患者和10例正常对照睾丸、精液中精子基因组未见基因微缺失.结论 AZFb区热休克转录因子基因的部分缺失将会使精子数量明显减少.     

An effective method for establishing animal models of azoospermia and oligospermia

The current study aims to develop a validated animal model to predict successful spermatogenesis retrieval in azoospermia and oligospermia men. Thirty-two mice were equally divided into 4 groups: control, scrotal hyperthermia (15 times), scrotal hyperthermia group (10 times), scrotal hyperthermia group (5 times). In the scrotal hyperthermia groups, their scrotum exposed to water at a temperature of 43°C for 20 min every other day. Then, the mice were euthanised and sperm samples were collected for sperm parameters analysis, and blood samples were obtained for hormonal assay. The testis samples were taken for histopathology experiments, immunofluorescence staining and Western blot in order to examine the protein expression together with RNA extraction in order to examine the gene expression of germ cell markers. The results of sperm analysis and histopathology of testicular tissue as well as the results of gene expression and Western blot showed that hyperthermia can significantly impair spermatogenesis. In conclusion, we have developed a novel model of azoospermia and oligospermia in mouse, which uses a high temperature to suppress spermatogenesis process through demolition of germ cells subsequent cell cycle arrest and apoptosis. The model will contribute to understanding azoospermia in human, oligospermia pathophysiology and the development of treatment.