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91.
Since the role of cutaneous nerves in the pathogenesis of cutaneous diseases associated with diabetes mellitus is not well defined, cutaneous nerves in ten patients with severe diabetic neuropathy were electron microscopically investigated as a preliminary study. The specimens were taken from normal-appearing skin of their lower extremities. Cutaneous nerves were seen as axon-Schwann cell complexes in which variously degenerated axons and Schwann cells coexisted with normal ones. The degenerative changes were not, however, specific for diabetes mellitus. 相似文献
92.
短串联重复序列HumFGA、D3S1359的法医学应用研究 总被引:1,自引:0,他引:1
对短串联重复序列(short tandem repeats,STR)的两个高多态位点HumFGA(human alpha fibrinogen,人类α-纤维蛋白原基因)、D3S1359的法医学应用进行研究,并通过实际检测案件统计结果进行评估。实验结果表明:两位点灵敏度高,分别为0.2、0.5ngDNA;同一性和可重复性均较理想;种属特异性好,常见动物未发现扩增产物,仅灵长类动物(黑叶猴和猕猴)有扩增条带;复合扩增体系效果良好;在352次减数分裂中,D3S1359未发现突变,HumFGA检测到1次,突变率为0.28%;实际案件统计和应用结果也显示两位点是多态性高,实用性强的两个遗传标记系统。HumFGA和D3S1359在法医学个人识别与亲子鉴定案例中有较大应用价值。 相似文献
93.
Active production of anti-human T-lymphotropic virus type I (HTLV-I) IgM antibody in HTLV-I-associated myelopathy 总被引:2,自引:0,他引:2
Kunihiko Nagasato Tatsufumi Nakamura Ohishi Kiyosumi Kohji Shibayama Masakatsu Motomura Ichinose Katsuhiro Mitsuhiro Tsujihata Shigenobu Nagataki 《Journal of neuroimmunology》1991,32(2):105-109
We investigated the presence of anti-human T-lymphotropic virus type I (HTLV-I) IgM in sera and cerebrospinal fluid from patients with HTLV-I-associated myelopathy (HAM) by Western blot analysis. Analyses of 36 serum samples revealed that most patients (31/36; 86.1%) had anti-HTLV-I IgM, whereas only four of 23 (17.4%) HTLV-I carriers had it. In studies of cerebrospinal fluid, anti-HTLV-I IgM was detected in 24 of 36 (66.7%) HAM patients, whereas none was detected in nine HTLV-I carriers. The differences were statistically significant (p less than 0.01). These results suggest that persistent active replication of HTLV-I occurs in the central nervous system as well as in the peripheral blood of HAM patients, and may contribute to the development of HAM. 相似文献
94.
H J Schluesener 《Journal of neuroscience research》1991,28(2):310-314
The transforming growth factors type beta 1, beta 2, and beta 1.2 suppress multidrug transport in human pat-1 glioblastoma cells and even in cells that strongly over-express mdr genes and are resistant to inhibition of multidrug transport by chemosensitizers. Thus, inhibition of multidrug transport by cytokines might be a new approach to increase cellular accumulation of chemotherapeutic agents in multidrug resistant glial tumor cells. Interestingly, a member of the more distantly related decapentaplegic subgroup of transforming growth factors, the bone morphogenetic protein BMP 2, did not inhibit multidrug transport. 相似文献
95.
肝癌患者HBV血清学标志和HBV-DNA的关系 总被引:2,自引:2,他引:0
目的 为了探讨肝癌患血清中HBV标志物与HBV-DNA之间的相关性。方法 运用酶联免疫吸附试验和聚合酶链式反应同时检测血清中HBV标志物和HBV-DNA。结果 在427例肝癌患的血清中,HBV-M(+)为74.0%,HBV-DNA(+)为44.0%,与正常对照组差异非常显。结论 乙型肝炎病毒的感染与复制仍是肝癌的主要致病因素。 相似文献
96.
97.
bFGF对人毛囊黑素细胞增殖、活化和黑素合成作用的研究 总被引:3,自引:0,他引:3
目的 研究碱性成纤维细胞生长因子 (bFGF)对人毛囊黑素细胞 (Hairfolloclemelanocyte ,HFM )增殖活化和黑素合成的影响。方法 体外培养正常人HFM ,观察不同浓度bFGF(0 3~ 1 2ng ml)对HFM形态、增殖、酪氨酸酶活性和黑素含量的影响。结果 bFGF处理HFM 7d后 ,HFM树突增多延长 ,酪氨酸酶活性和黑素含量增加显著高于对照组 (P <0 0 1) ,以 0 6ng ml组为著。结论 bFGF能诱导HFM增殖、酪氨酸酶活性和黑素合成增加 相似文献
98.
目的:研究大黄乙醇提取物的抗新城疫病毒(NDV)作用。方法:采用体外细胞培养的方法研究大黄乙醇提取物对NDV感染的拮抗作用。结果:20g/L剂量大黄乙醇提取物对BHK21细胞未有任何毒性作用;0.5g/L大黄对NDV所致CPE有明显的抑制作用;大黄可预防NDV感染,并对NDV的复制动力学有明显影响。结论:大黄乙醇提取物具有明显的抗NDV作用。 相似文献
99.
It has been previously demonstrated that the generation of measles virus (MV)-specific cytotoxicity (CTL) is reduced in patients with multiple sclerosis (MS). By contrast, CTL specific for influenza virus (FLU) and mumps virus is normal. It is uncertain if reduced CTL is limited to MV in MS patients, or if reduced CTL may be found to other viruses as well. Since MV-specific CTL is predominantly restricted by HLA class II molecules, while FLU-specific and mumps-specific CTL have large HLA class I-restricted components, reduced MV-specific CTL may reflect a broader reduction in HLA class II-restricted CTL in patients with MS. To examine this question we studied the generation of CTL specific for herpes simplex virus type I (HSV). HSV-specific CTL, like MV-specific CTL is predominantly restricted by HLA class II molecules. We found that patients with MS had reduced generation of CTL to both MV and HSV. Most, but not all patients who had reduced generation of CTL to one virus also had a similar impairment with respect to the second virus. Some patients, however, had a reduction in the generation of CTL only to MV or to HSV. These findings extend our earlier observations regarding reduced MV-specific CTL in patients with MS to a second HLA class II-restricted virus, HSV. Such a reduction may reflect discrete impairments in immune function to separate viruses, possibly those that are associated with viral persistence, or may reflect a more generalized defect in HLA class II-restricted CTL. 相似文献
100.
Irina Elovaara Ilkka Seppl Esko Kinnunen Hannu Laaksovirta 《Journal of neuroimmunology》1991,35(1-3):65-77
The presence of free immunoglobulin light chains (FLCs) in the cerebrospinal fluid (CSF) and sera of patients with human immunodeficiency virus-1 (HIV-1) infection, multiple sclerosis (MS), and neurologically healthy control individuals was investigated by paying special attention to ensure that only truly free light chains would be detected. The FLCs were extracted by specifically binding them to Sepharose-coupled anti-FLC monoclonal antibodies, and thereafter they were electrophoresed and immunoblotted with monoclonal antibodies to both light chain (LC) isotypes. A frequent occurrence of kappa and lambda FLCs was found in both CSF and sera of HIV-1 infected patients. In HIV-1 infection and in MS, the frequency of FLCs of the CSF was equal. In healthy controls, only occasional weak FLCs were observed in either CSF or serum. FLC bands of the CSF from patients with HIV-1 infection tended to be more intensive than those of the appropriately diluted sera. Both intrathecal synthesis of FLCs and their transudation from sera through the impaired blood-brain barrier (BBB) may contribute to this. Increasing severity of general HIV-1 infection was accompanied by an increase of FLC intensity in sera. A qualitative demonstration of FLC in the CSF may be meaningful only in the absence of altered BBB function. 相似文献