面部凹陷自体脂肪颗粒填充术的临床分析

目的探讨自体脂肪颗粒注射填充矫正面部凹陷畸形的临床效果。方法首先在患者脂肪丰厚的部位,注射器抽取脂肪颗粒,经清洗液反复冲洗后,将脂肪颗粒分点多层注入皮下。结果本组85例患者,55例注射1次,25例注射2次,5例注射3次。72例随访6~24个月,3例上下睑凹陷出现局部不平整,2例患者出现无菌性炎症,其余效果满意。结论面部凹陷畸形采用自体脂肪颗粒填充矫正,方法简单,安全有效。     

腰椎板棘突复合体回植椎板交叉螺钉固定重建腰椎椎管的研究

目的探讨腰椎板棘突复合体回植椎板交叉螺钉固定在腰椎后路手术中的应用价值及疗效分析。方法1998年6月-2006年11月,800余例腰椎疾患患者中168例行腰椎后路减压手术时将椎板棘突复合体完整切下后保留,减压完成后将椎板棘突复合体回植,用交叉螺钉固定。随访137例,随访时间为6～88个月,平均23．6个月。影像学研究通过复查X线片、CT和／或三维重建进行。临床疗效按JOA评分评定。结果回植骨块位置良好,椎板螺钉固定佳。椎管内无明显狭窄,椎间小关节融合或椎板间骨性连接123例,骨融合率为89．78％,临床优良率为90．5％。结论此术式可以重建腰椎椎管,固定可靠,从而有效防治术后椎管内的瘢痕黏连。     

Persistence and survival of autologous muscle derived cells versus bovine collagen as potential treatment of stress urinary incontinence

PURPOSE: We explored the use of autologous muscle derived cells as a method of treating stress urinary incontinence. We determined whether urethral muscle derived cell injection is feasible and compared it with bovine collagen injection. MATERIALS AND METHODS: Muscle derived cells isolated from female Sprague-Dawley rats were first transduced with retrovirus carrying the transgene for beta-galactosidase. We injected approximately 1 to 1.5 x 106 cells into the bladder wall and proximal urethra of 6 autologous animals. Tissue was harvested after 3 and 30 days, sectioned, stained for fast myosin heavy chain and assayed for beta-galactosidase. To compare muscle derived cell and bovine collagen injections 100 microl. of commercially available bovine collagen were also injected in Sprague-Dawley female rats. Tissue was harvested in 3 animals each after 3 and 30 days, sectioned and stained for trichrome. Subsequently, 3 adult SCID mice were used to compare the level of transgene expression at each time point after injecting 1.5 x 106 cells per injection, which were transduced with adenovirus carrying the transgene for beta-galactosidase. RESULTS: A large number of cells expressing beta-galactosidase were observed in the bladder and urethral wall 3 and 30 days after autologous cell injection in Sprague-Dawley rats. The persistence of primary muscle derived cells at 3 days was similar to that of collagen. However, at 30 days there was significant cell persistence while only a minimal amount of injected bovine collagen was detectable. Approximately 88% of the beta-galactosidase expression at day 3 remained at day 30 in SCID mice. CONCLUSIONS: We present 2 new findings important for the emerging field of urological tissue engineering, including the feasibility of injecting autologous skeletal muscle derived cells into the lower urinary tract and the greater persistence of such injected cells versus injected bovine collagen. Therefore, autologous muscle derived cell injection may be an attractive alternative treatment option for stress urinary incontinence.     

自体骨髓移植治疗股骨头无菌性坏死

Objective To investigate the effectiveness and mechanism of transplantation of autol-ogous bone marrow in treating femoral head necrosis. Methods Sixty New Zealand rabbits were randomly divided into groups A,B,and C after establishment of the models of femoral head necrosis. The left femoral head served as the control one,and the right as the experimental group. The mitoxantrone (0.1 mg/kg) with the help of DSA was injected into the femoral head of group A. One ml autologous bone marrow was injected into the femoral head of group B. In group C,mitoxantrone (0. 1 mg/kg) was injected,and 72 h later,1 ml autologous bone marrow was injected. Four months later,all rabbits were killed,and the femoral heads were removed and observed histologically and electron microscopically. Results The number of necrotic osteoclasts in groups A and B showed no significant difference ( P > 0.05 ), ande in group C, the number of necrotic femoral heads at the left and fight sides was 40. 60±4.11 and 21.33±2.16 respec-tively ( P < 0.05 ). At the experimental side of group C, the structure of majority bone cells was clear and intact,and necrosis was occasionally seen. Conclusion At the cellular level, local chemotherapy and au-tologous hematopoietic stem cell transplantation had certain effectiveness for aseptic necrosis of the femoral head.     

自体输血在骨科手术中的应用

我院自1999年3月开展自体血回输技术,明显减少了异体输血用量,未出现明显不良反应,现报告如下. 1 临床资料 本组275例,男176例,女99例;年龄17～82岁,平均68岁.     

自体软骨细胞修复关节软骨缺损的机制探讨

目的:观察团块样自体软骨细胞植入关节软骨缺损后的病理变化,探讨自体软骨细胞移植修复关节软骨缺损的病理生理机制。方法:24只3.0kg以上4～6月龄新西兰大白兔,雌雄不限,随机分为两组:实验组和对照组。实验组12只,20%的速眠新(1mg/kg)肌肉注射麻醉后取肩关节软骨组织,0.2%Ⅱ型胶原酶消化分离软骨细胞,体外单层培养,细胞长成肉眼可见的膜状后收集固体的组织样细胞团,动物再次麻醉制造双膝股骨滑车4.0mm×6.0mm方形缺损,植入细胞团块,骨膜覆盖,缝合骨膜于双股骨髁上。对照组12只,同实验组手术方法进行缺损单纯骨膜移植。1、3、12、24周两组各3只动物空气栓塞处死取材,观察细胞团块变化和缺损修复情况。结果:1周时软骨细胞朝向关节面部分细胞变大变圆,产生大量基质;3周时此种变化更加明显,但骨膜与细胞团块已然不能分开;12周时缺损为类透明软骨组织修复;24周时修复组织为透明软骨样组织,对照组为纤维软骨组织修复。结论:关节软骨细胞体外聚集培养形成的细胞团块内的细胞有迁移生长能力;细胞团块移植方法植入的细胞数量大,表型好,细胞在缺损内不会流失;关节软骨缺损修复是由植入的细胞团块生长分化而来;自体关节软骨细胞团块植入关节缺损内后,在关节应力的影响下,先从朝向关节面的一侧逐渐发生细胞成熟分化和软骨基质产生,逐渐完成缺损的修复。     

自体肺动脉组织一期矫治主动脉弓病变合并心内畸形

目的 探讨自体肺动脉组织一期矫治主动脉弓病变合并心内畸形的手术方法和治疗效果.方法 1993年4月至2009年11月,共25例主动脉弓病变合并心内畸形的患儿接受了一期矫治手术.男性17例,女性8例;年龄26 d～6.5岁,平均11个月;体质量3.0～14.5 kg,平均12.4 kg.其中6例患儿为主动脉弓缩窄,6例为主动脉弓中断,13例为主动脉弓发育不良.手术技术包括切开肺动脉前壁,切除动脉导管,应用部分自体肺动脉壁重建主动脉弓,重建肺动脉,并同时矫治合并心内畸形.结果 24例患儿术后恢复顺利,1例患儿死于围手术期肺动脉高压危象.随访心脏超声及心脏CT检查证实主动脉弓重建及心内畸形矫治满意.术后未发生神经系统及其他并发症.随访过程中,患儿生长发育正常,主动脉弓无再狭窄.结论 自体肺动脉具有生长潜力且柔韧性好,是一种理想的重建主动脉弓的材料.一期矫治手术治疗先天性主动脉弓病变合并心内畸形可以得到满意的结果.     

自体脂肪颗粒移植鼻根局部隆鼻术

目的：探讨自体脂肪颗粒移植在隆鼻术中的临床应用。方法：针对一组10例单纯鼻根低平的就医者,注射自体脂肪颗粒进行隆鼻。结果：8例就医者1次充填即取得预期临床效果,2例就医者经过再次手术填充达到满意效果。结论：应用自体脂肪颗粒移植鼻根局部隆鼻能够达到良好、稳定的临床效果。     

自体腓骨移植治疗股骨下1/3骨折伴大段缺损

<正>股骨下1/3骨折伴大段缺损常由高能量暴力所致,骨折较复杂,临床治疗难度较大。异体骨移植、人工替代物置换、骨延长转移术虽都已在临床上得以应用,但都存在各自的局限性[1-4]。我院自2003年7月至2007年4月,采用自体腓骨游     

Nonanemic Patients Do Not Benefit from Autologous Blood Donation Before Total Hip Replacement

To avoid the potential risks of allogeneic transfusion during total hip arthroplasty (THA), the use of preoperative autologous blood donation (PABD) has been utilized. We performed a retrospective chart review of 283 patients undergoing THA that either donated 1 U of autologous blood (188 patients) or did not donate autologous blood before surgery (95 patients) in order to investigate the difference in postoperative transfusion rate (autologous and allogeneic), the incidence of allogeneic transfusion, and the difference in cost of each protocol. In addition, the study compared transfusion rates in patients with and without preoperative anemia (hemoglobin (Hb) ≤ 12.5 g/dL). At 0.75 transfusions per patient versus 0.22 transfusions per patient, the PABD patients had a significantly higher overall transfusion rate. PABD significantly reduced the need for allogeneic blood in anemic patients (Hb ≤ 12.5 g/dL) from 52.6% to 11.8%. PABD did not have the same affect in nonanemic patients (allogeneic transfusion rate 5.7% versus 4.0%). The study demonstrated that nonanemic patients undergoing THA do not benefit from PABD, but it is effective for anemic patients.