Healing of the guinea pig incisor after partial pulp removal

Abstract A histological and microradiographical study was made of eruption and healing of guinea pig lower incisors after endodontic treatment compared with intact incisors. One incisor per animal was cut perpendicularly at the level of the gingiva. One third to one half of the pulp was removed. The experimental periods were 0, 4, 7, 16, 21 and 31 days. Unstained, HE or Brown and Brenn stained sections were studied microscopically. Some teeth were sectioned undecalcified and studied microradiographically. In intact incisors the dentin matrix deposition and mineralization were described. In the dentin, incisally to the pulp proper, narrow canals, the walls of which consisted of mineralized material, were found opening at the occlusal surface. Histologically, in the treated teeth, a beginning of cell-rich fibrodentin deposition at the original dentin wall was present at Day 4. At Day 7 a substantial walling off of the pulp is achieved by cell-rich and some cell-poor fibrodentin. After 16 days large amounts of cell-rich and cell-poor fibrodentin were covered by some tertiary dentin. After 31 days the amount of tertiary dentin was larger than after 16 days and also covered the incisal part of the root canal walls. Further apically some vasodentin and newly formed normal, secondary, dentin was observed. In course of this period the cells of the cell-rich and cell-poor fibrodentin first showed necrobiosis, then necrosis. The cell-rich fibrodentin appeared not fully mineralized. All blood vessels and strands of soft tissue which ran in canals in the fibrodentin came to a dead end. Thus the root canal was fully obturated. Although the teeth appeared (nearly) normal to the naked eye and radiographically after 31 days, histologically they were not. Large amounts of fibrodentin still being present incisally which showed the dual potential of the guinea pig pulp to produce both fibrodentin and orthodentin.     

内皮细胞生长因子表达质粒的构建及其促进烫伤愈合的研究

目的　构建血管内皮细胞生长因子 (VEGF)的表达质粒 ,并观察其表达产物对小型猪深度烫伤创面影响。方法　应用逆转录 聚合酶链式反应 (RT PCR)技术克隆VEGF基因的cDNA序列 ,并将其正向连接到表达载体pQE 40上 ,通过十二烷基硫酸钠 聚丙烯酰胺凝胶电泳(SDS PAGE)和蛋白质印迹方法对其表达产物进行特异性鉴定。猪小面积深Ⅱ度烫伤后 ,立即将含有 1 μg/ g的VEGF膏剂均匀涂于创面 ,每个创面 2 0 0mg膏剂 ,隔日换药 1次 ,至伤后 1 1d。用透明膜描记称量法记录创面面积 ,并取创面组织作组织学检测。结果　克隆出的VEGF基因cDNA片段由 380bp组成 ,包括翻译起始密码子、编码序列和终止密码子等部分。重组表达质粒 pQE VEGF在M1 5细菌体内能够表达 ,VEGF主要存在于细菌包涵体内。小型猪烫伤后 7d,VEGF处理的伤口处肉芽组织增生明显 ,毛细血管数量增多。烫伤 1 1d后 ,VEGF组的创面可见连续的上皮覆盖 ,而M1 5组的创面面积为 (0 .71± 0 .2 3)cm2 ,伤口仍未愈合 ,两者差异有非常显著性 (P <0 .0 1 )。结论　构建的VEGF表达载体可以编码VEGF1 2 1蛋白 ,其表达产物能够诱导肉芽组织生长 ,加速新生血管形成 ,促进创面愈合     

袜套持续外展牵引法整复难复性肩关节脱位

1988年1月～1999年6月,我们应用袜套持续外展牵引法,成功整复难复性肩关节脱位32例.其中2例来院就诊时间超过48小时. 1 临床资料 本组32例,男27例、女5例,年龄18～62岁.前脱位31例,后脱位1例,就诊时间6～58小时不等.其中18例在院外多次复位失败而转入我院就诊.14例为在我院采用Hippocrates及Kocher等方法复位失败者.32例中合并肱骨大结节骨折3例,同侧肋骨骨折2例,伤前患有冠心病者4例.     

重组人表皮生长因子凝胶对动物腹部手术切口抗张力强度影响的实验研究

目的 :探讨重组人表皮生长因子 (rh EGF)凝胶对动物腹部手术切口的修复作用。方法 :实验分两部分进行。第 1部分 :SD大鼠 90只 ,随机分为 3组 (切口内外用药组、切口外部用药组和空白基质对照组 ) ,每组 30只 ,每日切口处涂药 1次。术后第 4、5、6、8、10日每组分别处死 6只动物 ,取切口处皮肤进行拉力试验并进行病理组织学检查。第 2部分 :实验用小型猪 6头 ,设 4个用药组 (切口内外用 rh EGF凝胶组、外用 rh EGF凝胶组、外用 rh EGF水剂组和空白基质对照组 ) ,采用自身对照 ,用药方法同上 ,术后第 5、7、9日各处死 2只动物 ,取切口处皮肤同上法测定和检查。结果 :rh EGF凝胶外部用药组两种动物切口处皮肤抗张力强度及拉伸率在不同的用药时间内较空白基质对照组均明显增强 (P<0 .0 5或 P<0 .0 1) ;内外用药组的抗张力强度明显低于外部用药组 (P<0 .0 5或 P<0 .0 1) ,且切口处炎性细胞弥漫性浸润 ,并见异物巨细胞反应 ;外用 rh EGF水剂组抗张力强度和拉伸率与 rh EGF凝胶内外用药组相当 ,但低于 rh EGF凝胶外部用药组。结论 :rh EGF凝胶可明显增强动物手术切口皮肤抗张力强度 ,提高手术切口的愈合质量 ,加速手术切口的愈合。rh EGF凝胶不同的用药方式及 rh EGF不同的剂型对动物皮肤切口抗张力强度有一定     

Effect of ciprodex on graft healing in tympanoplasty

The present study was conducted to evaluate whether the administration of ciprofloxacin 0.3%/dexamethasone 0.1% (Ciprodex)-soaked gelfoam during tympanoplasty has adverse effects on graft healing. Records of patients who had undergone tympanoplasty with Ciprodex-soaked gelfoam packing placed in the middle and external ear canal were reviewed. The time to heal for each patient and the number of postoperative perforations/complications were recorded. Sixty-four charts met the inclusion criteria. Most procedures were primary type I tympanoplasties with temporalis fascia grafts. Healing of the tympanic membrane was documented in 95.3% of patients, and mean time to healing was 49 d. Two patients who underwent revision tympanoplasty failed to exhibit healing at any visit after surgery. Postoperative complications were infrequent. Patients with Ciprodex-soaked gelfoam packing placed during tympanoplasty showed an overall rate of healing of 95%. Although this study is limited by its retrospective design, the data suggest that the use of Ciprodex during tympanoplasty has no detrimental effect on postoperative graft healing.     

自体邮票植皮术联合冲洗治疗在烧伤患者中的疗效观察

目的分析自体邮票植皮术联合冲洗治疗在深度烧伤患者中的治疗效果。 方法选取新疆医科大学第八附属医院2015年1月至2019年1月收治的68例大面积烧伤患者,随机数字表法分为2组：对照组与观察组,每组34例。对照组Ⅰ期行清创削痂手术、自体邮票植皮术治疗;观察组在此基础上每日联合进行创面冲洗治疗持续至自体邮票植皮术手术前夕,后行邮票植皮术,观察2组手术时间,Ⅰ期创面愈合率、康复率,以及创面分泌物细菌培养阳性率,术后3、7、15 d植皮成活率以及创面愈合时间,评估患者治疗前、治疗后3个月以及治疗后6个月视觉模拟评分法(VAS)评分。数据比较采用t检验。 结果观察组手术时间[(70.31±7.69) min]略长于对照组[(66.52±8.41) min],差异无统计学意义(t＝1.939, P>0.05),观察组Ⅰ期创面愈合率、康复率[(77.34±7.05)%、(74.27±5.84)%]明显高于对照组[(65.61±6.92)%、(63.41±3.35)% ],且创面分泌物细菌培养阳性率[(21.19±4.42)%]低于对照组[(64.36±11.25)%],差异均有统计学意义(t＝ 6.924、9.406、20.826, P<0.05)。术后3、7、15 d,观察组植皮存活率[(34.11±5.26)%、(59.31±4.72)%、(3.19±1.42)%]均高于对照组[(28.64±11.45)%、(51.74±5.68)%、(64.71±8.25)%],差异均有统计学意义(t＝2.531、5.977、3.717,P<0.05),且观察组创面愈合时间[(3.19±1.42) d]短于对照组[(5.36±2.25) d],差异有统计学意义(t＝4.756, P<0.05)。治疗前2组VAS评分差异无统计学意义P>0.05),治疗后3个月、6个月,观察组VAS评分[(5.41±0.34)、(3.35±1.09)分]分别低于对照组[(6.15±1.28)、(4.37±1.24)分],差异均有统计学意义(t＝3.258、3.603,P<0.05)。 结论大面积烧伤患者应用邮票植皮术联合进行创面冲洗治疗可有效改善手术指标,提高植皮存活率,对疼痛缓解也有一定帮助。     

防疤烧伤膏对大鼠伤口愈合的影响

目的：观察防疤烧伤膏对大鼠创面修复的影响。方法：采用大鼠皮肤切割伤模型。创面外用防疤烧伤膏，并采用碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF)和空白处理作为阳性和阴性对照。透明膜标记称量法和注水法记录伤后3d,7d和14d创面面积和伤腔容积，伤后7d和14d取创面组织进行组织学检查。结果 防疤烧伤膏能够减轻伤口炎症反应，促进坏死组织脱落，缩短伤口愈合时间。结论：外用防疤烧伤膏对创面修复有促进作用。