Mesothelioma of the tunica vaginalis testis with prominent adenomatoid features: a case report

Malignant mesotheliomas of the testis arise from the tunica vaginalis, formed from the evagination of the abdominal peritoneum into the scrotum. It is an extremely rare tumor representing 0.3% to 5% of all malignant mesotheliomas. We presented an interesting case of 68-year-old male with swelling and slightly painful in the right scrotum. Histologically, the lesion were composed of small tubular, microcystic, gland lined by flattened epithelioid cells and vague signet ring cells set in a myxofibrous stroma, which is resemblance to adenomatoid tumor. But the tumor cells showed significant atypical cytologic morphology and invaded into spermatic cord tissue, which indicated the diagnosis of malignant tumor. Immunohistochemistry study showed positive expression of CK, CK5/6, CK7, Calretinin, D2-40 and Vimentin which indicated the diagnosis of malignant mesothelioma. This case of mesothelioma should be classified as epithelial in type. To our knowledge, the mesothelioma of the tunica vaginalis testis with adenomatoid tumor-like microscopic features is very rare.     

阴道毛滴虫Rab6鸟苷三磷酸酶同源基因的cDNA克隆和序列分析

Rab鸟苷三磷酸酶是细胞膜转运机制的关键调节分子,与原虫的分泌功能密切相关。本研究旨在克隆和分析阴道毛滴虫Rab6鸟苷三磷酸酶同源基因,以便进一步探讨其功能。作者从阴道毛滴虫cDNA表达文库中分离出2个Rab6鸟苷三磷酸酶同源基因的cDNA克隆,其中1个cDNA序列长658对碱基,读码框含597对碱基,推测蛋白质序列具198个氨基酸。序列分析表明该氨基酸序列与Rab6a蛋白亚家族的同源性最高。另一cDNA序列长764对碱基,读码框含657对碱基,推测蛋白质序列具218个氨基酸。序列比对分析显示该氨基酸序列与Rab6b蛋白有较高的同源性。2个氨基酸序列都拥有Rab鸟苷三磷酸酶家族的所有保守结构域、特异性RabF结构域和典型的异戊烯化结构域。进化树分析提示毛滴虫的这2个基因系Rab6家族的同源基因,在进化上更接近原虫和单细胞的酵母Rab6亚家族。序列分析还显示这2个基因都无内含子,其基因组DNA序列与其cDNA序列完全一致。     

阴道毛滴虫致男性不育的机制研究

目的调查了解男性泌尿生殖系阴道毛滴虫（Tv）感染情况与不育的关系,及发生机制的研究。方法涂片、瑞-姬（W—G）染色和培养用光学双目显微镜观察了1986例精液内Tv及精子的活率、活力等指标;采用肝-胨-糖培养方法,对Tv体外培养后,除去虫体,留取培养液,稀释成3个不同浓度,将10例正常生育男性的优化精子分为等体积的（A、B、C、D）4组：A组加入未培养Tv的原培养液,B、C、D组依次加入（1．2×10^8／L、6×10^8／L、1．2×10^9／L）梯度培养液,分别于37％水浴箱中孵育0．5、1、2、4h观察精子运动参数。结果Tv检出率为38％,Tv感染者精子运动参数明显低于正常生育者（P〈0．01）。Tv培养液浓度在6×10^8／L以上时,精子的活率和活力与对照组相比均显著降低（P〈0．05）,并呈浓度时间依赖性。结论Tv对精子运动能力有明显的损害作用及代谢产物是其主要的损害抑制因素,是造成不孕不育的重要原因之一。     

Acute pancreatitis masquerading as testicular torsion

A 40-year-old man presented with fat necrosis of scrotum as the complication of acute pancreatitis. Excessive fluid accumulation in the pancreas and the extrapancreatic spaces, including around the spermatic cord, was seen on computed tomography. Surgical specimen showed typical fat necrosis of tunica vaginalis and the spermatic cord. After the surgery, pain of the testicle subsided completely, without recurrence. From the clinical presentation alone, it had been difficult to differentiate this patient's condition from torsion of the spermatic cord.     

无症状及复发性细菌性阴道病的诊治

细菌性阴道病(bacterial vaginosis,BV)是阴道内厌氧菌混合感染,由正常菌群失调引起,是育龄期女性最常见的下生殖道感染性疾病[1-2]. 现如今阴道微生态的诊断评价体系已相对完善,国内外均有针对BV诊治的相关指南以指导临床治疗,但是对于特殊状态下BV的处理,如无症状性BV及复发性BV,临床实际工作中经...     

低温保存阴道毛滴虫的实验观察

利用5%、10%、15%、20%、25%、30%浓度的二甲基亚砜和甘油肝浸汤培养液对临床分离的阴道毛滴虫进行4℃保存观察。二甲基亚砜和甘油两实验组最适保种浓度分别为15%和10%,50%虫体存活率天数分别为29和27 d,最长保存天数分别为31和29 d;而空白对照组仅为5和7 d。结果表明,15%二甲基亚砜或10%甘油肝浸汤培养液4℃冷藏保存阴道毛滴虫可显著延长其保存期。     

Trichomoniasis treatment in women: a systematic review

objective  To compare the effectiveness of various treatment strategies for trichomoniasis in women.
data sources  Medline from 1966 to1996, Embase from 1986 to 1996, Science Citation Index from 1990 to 1996; reference lists of existing reviews; through the manufacturers of metronidazole and tinidazole in the UK, the Cochrane Controlled Trials Register until October 1997 and informal discovery.
study selection  Any randomized or quasi-randomized trial in nonpregnant women with trichomoniasis where different treatment strategies were compared. 45 of the 124 identified studies met the criteria and were included in the review.
data extraction  Settings, diagnostic methods, exclusions, loss to follow-up and partner treatment strategies were extracted. Outcomes sought were parasitological cure, clinical cure and side-effects of treatment.
results  Most trials were small, with only two trials containing more than 100 women in each comparison group. Only 11 trials followed up women for more than one month. Oral nitroimidazoles were effective in achieving parasitological cure. Fourteen trials compared different treatment strategies with the remainder comparing different doses or different drugs. Partner treatment was effective in decreasing longer-term re-infection rates in the one trial testing this.
conclusions  Parasitological cure can be achieved by a single oral dose of nitroimidazoles. There is, however, very little data on partner treatment strategies and long-term cure rates after initial treatment. Further research should test various partner treatment strategies to prevent re-infections and reduce trichomoniasis prevalence.     

Immunohistochemical study of the vaginal inflammatory response in experimental trichomoniasis

In the present paper, the acute and subchronical inflammatory processes of the vaginal epithelial were studied in mice experimentally infected with two Trichomonas vaginalis strains of different pathogenicity, by means of histological and immunological methods. There was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. The proliferation of the vaginal epithelium favors the settlement and persistence of the parasitic infection. All of the findings corresponded with signs of a systemic disease being observed in the animals, including significant weight loss and also intestinal invasion. The entire inflammatory process has been corroborated by studies of adhesion molecules such as E-Selectin, VCAM-1 and PECAM-1. A correlation between the time of appearance and the perseverance of the inflammatory process with E-Selectin and VCAM-1 expression was observed, but not with PECAM-1. The strain with a higher pathogenicity was able to invade deep vaginal tissues and thus, parasites could not be detected by vaginal washings. This may be an important cause of diagnosis and treatment failure. Also, by the different localization of trichomonads, it appeared that the battle between host and parasite took place in different areas dependent upon the characteristics of the strain.