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61.
62.
The Use of Stent Grafts for the Treatment of Aneurysms and Dissections of the Thoracic Aorta: A Single Centre Experience 总被引:5,自引:0,他引:5
P. Matravers R. Morgan A. Belli 《European journal of vascular and endovascular surgery》2003,26(6):587-595
OBJECTIVE: To review the results of stent graft treatment for diseases of the thoracic aorta. DESIGN: A retrospective study of the results of thoracic stent graft procedures in a single centre. METHODS: The results of 30 procedures performed in 24 patients were analysed. Eleven patients had aneurysmal dilatation of the descending thoracic aorta (>5.5 cm), nine patients had complicated type B dissection, three had penetrating ulcers and one had a traumatic pseudoaneurysm. Imaging follow-up consisted of CT scans performed at one, three, six and 12 months following the procedure and annually thereafter. RESULTS: One technical failure occurred due to a ruptured external iliac artery. There were no other immediate failures. The primary technical success rate was 83%. The 30-day procedural and patient mortality rates were 7 and 8%, respectively. No neurological complications occurred. Seven patients had the subclavian artery covered without complications. Secondary intervention was required in 21%. During the follow-up period (mean 11 months, range 1-48 months.) there were five deaths, two of which were related to stent graft infection. CONCLUSION: Thoracic stent grafts offer a realistic alternative to surgery. 相似文献
63.
成骨细胞与血管内皮细胞联合培养的生物学特性 总被引:7,自引:1,他引:6
目的探讨成骨细胞与血管内皮细胞联合培养的生物学特性. 方法取2周龄乳兔颅盖骨及肾脏皮质传代培养制备成骨细胞(A组)、血管内皮细胞(B组)及成骨细胞与血管内皮细胞联合培养(C组),用Ⅰ型胶原和血管Ⅷ因子免疫细胞化学染色鉴定成骨细胞和血管内皮细胞,倒置相差显微镜和组织学染色观察细胞的生长特性和细胞相容性,检测碱性磷酸酶 (alkaline phosphatase,ALP)活性,观察血管内皮细胞对成骨细胞产生的ALP活性有无影响,MTT法检测细胞活力,分析细胞生长和增殖情况. 结果免疫细胞化学染色证实,培养的细胞为成骨细胞和血管内皮细胞.倒置相差显微镜、HE和Masson染色均显示两种细胞混合生长良好.ALP检测结果:C组ALP活性明显高于A组和B组(P<0.01),A组高于B组(P<0.05).MTT检测结果表明:C组细胞早期增殖较慢,而后期增殖较快. 结论成骨细胞与血管内皮细胞具有良好的相容性,血管内皮细胞能够增强成骨细胞的ALP活性,提高成骨细胞的增殖能力.联合培养细胞具有很强的增殖潜能. 相似文献
64.
对197例拇指Ⅲ~Ⅳ级缺失者,采用自体姆甲瓣游离移植包裹冷冻异体手指的复合组织(骨、关节、肌腱、腱鞘)再造拇指200指。笔者认为冷冻能降低异休手指组织的抗原性,术前、后不用抑制排异反应的制剂与激素,无急性排异反应发生。手术成功率96.4%,异体手指存活率91.5%。术后3个月感觉恢复,测定对掌功能144例,优良率为91.6%。平均随访时间4年5个月,部分病例远期出现异体骨吸收及关节变性,可能与骨片未镶嵌好,术后活动过早及慢性排异有关;但主要是移植组织未及时恢复神经血管的营养支配有关。为此取■甲瓣时内附着趾关节2块骨片,挖去异体指骨内骨髓,植入自体新鲜红骨髓及松质骨,有利于血管长入异体骨及促进宿主对其爬行替代。提出合理设计供足阳趾胫侧的保留皮瓣,尽量维持趾长度,供皮瓣区中厚皮片植皮,保存第1背侧跖骨肌及跗内外侧血管及足背皮神经,可减少足的并发症。 相似文献
65.
66.
67.
微量沙眼衣原体组织培养法的建立及临床应用 总被引:8,自引:0,他引:8
建立了微量沙眼衣原体组织培养法,并应用于不同人群的生殖道标本的沙眼衣原体培养。结果生殖道沙眼衣原体培养的阳性率:男性尿道炎患者147%(9/61);正常孕妇33%(7/210);急性和亚急性盆腔炎女性患者67%(7/104);性病门诊患者182%(49/269)。由于96孔培养板及倒置荧光显微镜的应用,微量培养法试剂用量少、过程简化;自制的抗沙眼衣原体单克隆抗体大大降低了培养后的染色成本,适用于大宗标本。 相似文献
68.
W. Gubisch 《European journal of plastic surgery》1993,16(3):125-129
Summary Traumatic defects of the auricle are quite common. The eventual success of the repair is considerably influenced by the initial treatment. The different parts of the auricle require different approaches. This paper presents our methods for repairing damage to the cartilaginous frame using autologous ear cartilage grafts, and local flaps from the mastoid region to cover soft tissue defects. 相似文献
69.
Inhibition of tissue factor surface expression in human peripheral blood monocytes exposed to cytokines 总被引:1,自引:0,他引:1
Interleukin (IL)-4, IL-10, IL-13 and transforming growth factor beta (TGF-β) are known to regulate several monocyte functions, including inhibition of the synthesis of different cytokines. Using quantitative RT-PCR and flow cytometry analysis we investigated the effects of these cytokines on bacterial lipopolysaccharide (LPS)-induced tissue factor (TF) expression in human monocytes. The effects of IL-4 and IL-10 on monocyte chemoattractant protein-1 (MCP-1)- and C-reactive protein (CRP)-induced TF expression were also studied. A direct comparison revealed that IL-4, IL-10 and IL-13 all down-regulated LPS-induced TF expression in a concentration-dependent manner without the need for priming. In contrast, TGF-β required 4 h of priming to inhibit TF expression induced by LPS. IL-10 was the most powerful inhibitor, causing almost complete inhibition at 5 ng/ml. IL-4 and IL-13 exhibited a significantly lower inhibitory capacity even at concentrations of 100 ng/ml. IL-4 and IL-10 showed similar concentration-dependent inhibition of MCP-1- and CRP-induced TF expression. We also showed that the regulatory effect of the interleukins occurred at the mRNA level. In vivo , these inhibitory cytokines may play an important regulatory role in preventing thrombosis. IL-10, in particular, may be a possible candidate as a TF-preventing drug. 相似文献
70.
Ultraviolet Laser-Induced Fluorescence Spectroscopy Diagnosis of Human Stomach Malignant Tissues 总被引:2,自引:0,他引:2
To evaluate the potential of laser-induced autofluorescence spectroscopy for the detection of premalignant lesions of human
stomach, fluorescence properties of stomach tissues have been investigated in vitro and in vivo. A specially made optical
fibre probe and the multichannel fluorescence collection system have been used successfully in our research.
Paper received 26 June 1997; accepted in revised form 31 October 1997. 相似文献