角蛋白酶的研究进展

本文概述了角蛋白酶的理化性质、降解机理,并综述了近年来其应用领域及国内外研究进展情况。     

Incorporating PCA and fuzzy-ART techniques into achieve organism classification based on codon usage consideration

To recognize the DNA sequence and mine the hidden information to achieve the classification of organisms are viewed as a difficult work to biologists. As we know, the amino acids are the basic elements to construct DNA. Hence, if the codon usage of amino acids can be analyzed well, the useful information about classification of organisms may be obtained. However, if we choose too many amino acids to perform the clustering analysis, the high dimensions also lead the clustering analysis to be a complicated structure. Hence, in this study, we will incorporate the principle component analysis and fuzzy-ART clustering techniques into constructing an integrated approach. The useful information about organisms classification based on the codon usage can be mined by using the proposed approach. Finally, we also employ a case including 18 bacteria to demonstrate the rationality and feasibility of our proposed approach.     

医学科研设计中的随机化问题

目的:引起科研工作者在医学科研设计中对贯彻随机化原则的重视,进一步提高科研的质量. 方法:由医学科研设计中一些实际案例的分析入手,提出了随机化的内涵、要求及常见问题.结果:医学科研中由于随机化设置存在很多问题,影响了研究结论的科学性、可靠性. 结论:随机化原则是医学科研设计中非常重要的原则,在科研过程中必须认真贯彻.     

主成分分析法在核心期刊综合评价中的应用

核心期刊的评价方法多采用综合评价法，但这些综合评价法都存在着一定的局限性。现提出用主成分分析法对中文期刊进行评价，以探讨一种新的核心期刊评价方法，并对评价核心期刊的主要影响因素及各种因素的权重作进一步分析。     

突发公共卫生事件时检伤分类原则的伦理学研究

检伤分类是对伤员在突发公共卫生事件且医疗资源受限的条件下实施有效救治的重要环节之一。从伦理学角度探讨了检伤分类的公平原则、功利原则和科学决策原则,以此来保证检伤分类的快速准确。     

折叠输液管对小儿头皮静脉穿刺的效果观察

目的探讨折叠输液管产生负压来提高小儿头皮静脉穿刺成功率的临床效果。方法将200例头皮静脉穿刺的患儿随机分成对照组和观察组各100例,对照组采取常规静脉穿刺法,观察组采取折叠输液管法,于排气后将输液管末端折叠0．5cm,用夹子夹紧,当针头刺入皮下后松开夹子,使输液管内产生少量负压。观察2组患儿头皮静脉穿刺时的自然回血情况及穿刺成功率。结果对照组自然回血率72％,穿刺成功率87％;观察组自然回血率97％,穿刺成功率97％。观察组自然回血率和穿刺成功率均高于对照组。结论折叠输液管法能迅速判断针头进入血管,可有效提高静脉穿刺成功率。     

折叠输液管对小儿头皮静脉穿刺的效果观察

目的 探讨折叠输液管产生负压来提高小儿头皮静脉穿刺成功率的临床效果.方法 将200例头皮静脉穿刺的患儿随机分成对照组和观察组各100例,对照组采取常规静脉穿刺法,观察组采取折叠输液管法,于排气后将输液管末端折叠0.5 cm,用夹子夹紧,当针头刺入皮下后松开夹子,使输液管内产生少量负压.观察2组患儿头皮静脉穿刺时的自然回血情况及穿刺成功率.结果 对照组自然回血率72%,穿刺成功率87%;观察组自然回血率97%,穿刺成功率97%.观察组自然回血率和穿刺成功率均高于对照组.结论 折叠输液管法能迅速判断针头进入血管,可有效提高静脉穿刺成功率.     

Understanding the physiological functions of the host xenobiotic-sensing nuclear receptors PXR and CAR on the gut microbiome using genetically modified mice

Pharmacological activation of the xenobiotic-sensing nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) is well-known to increase drug metabolism and reduce inflammation. Little is known regarding their physiological functions on the gut microbiome. In this study, we discovered bivalent hormetic functions of PXR/CAR modulating the richness of the gut microbiome using genetically engineered mice. The absence of PXR or CAR increased microbial richness, and absence of both receptors synergistically increased microbial richness. PXR and CAR deficiency increased the pro-inflammatory bacteria Helicobacteraceae and Helicobacter. Deficiency in both PXR and CAR increased the relative abundance of Lactobacillus, which has bile salt hydrolase activity, corresponding to decreased primary taurine-conjugated bile acids (BAs) in feces, which may lead to higher internal burden of taurine and unconjugated BAs, both of which are linked to inflammation, oxidative stress, and cytotoxicity. The basal effect of PXR/CAR on the gut microbiome was distinct from pharmacological and toxicological activation of these receptors. Common PXR/CAR-targeted bacteria were identified, the majority of which were suppressed by these receptors. hPXR-TG mice had a distinct microbial profile as compared to wild-type mice. This study is the first to unveil the basal functions of PXR and CAR on the gut microbiome.     

驱白巴布期中微量元素与组方原理的关系研究

目的 通过测定驱白巴布期中与白癜风相关的微量元素含量,分析该药中微量元素分布与组方原理之间的关系.方法 采用HNO3-H2O2高压消解罐法消化处理样品,用电感耦合等离子体发射光谱法(ICP-AES)同时测定Cu,Zn,Fe,Ca,Mg,Mn,Sr,Co等8种微量元素,原子荧光法(AFS)测定Se元素.结果 两种方法 的RSD(n=5)在0.65%～3.18%内,元素回收率在90.52%～103.56%之间,测定结果 较理想.结论 ICP-AES法和AFS法测定微量元素方法 简便,快速准确,适于分析测定药材中的微量元素,结果 发现驱白巴布期中的微量元素含量与其组方原理有很大相关性,为今后进行基础研究及进一步开发提供信息.     

循证医学原理在药房管理中的应用价值研究

目的探讨分析循证医学原理在药房管理中的应用价值。方法 2012年6月-2013年6月选择本地区两家社区药房作为研究对象,分别为药房A和药房B,药房A采用循证医学原理进行经营管理,药房B采用常规的管理方法进行管理。比较一年后,两家药房的平均月客流量增长率和患者满意度。结果药房A的平均月客流量增长率为25.6%,高于药房B的-11.1%,差异有统计学意义（P〈0.05）。两家药房的患者满意度比较差异有统计学意义（P〈0.05）。结论采用循证医学原理进行药房管理,可大大提高药房的管理效率,提高药房的客流量和患者满意度,值得推广应用。