地方性氟中毒病区胎儿肾上腺皮质细胞超微结构体视学研究

对地方性氟病病区胎儿肾上腺皮质细胞进行超微结构体视学研究。结果显示：病区胎儿肾上腺皮质细胞核比表面和核平均体积缩小，细胞平均截面积和平均体积缩小；线粒体肿胀，平均周长和平均体积增大；粗面内质网体密度缩小。结果表明：机体过量氟对胎儿肾上腺皮质细胞超微结构有损伤作用。     

星体积及其在肺泡空间局部大小估计中的应用

星体积是反映任意结构(尤其是不规则结构)的(平均)局部大小的体视学新参数,可据点取截距测量进行无偏估计.星体积已用于定量研究骨小梁、骨髓腔以及胎盘绒毛间隙的大小;星体积在评价肺泡空间的(局部)大小中也非常有用.     

猕猴小肠5—羟色胺内分泌细胞的分布及形态学研究

以免疫组织化学链霉卵白素（ Ｓ Ｐ）法显示猕猴小肠５ 羟色胺（５ Ｈ Ｔ）内分泌细胞，并用 Ｗｅｉｂｅｌ体视学方法对其进行了定量分析。结果表明，此种细胞的密度在十二指肠最高，空肠密度中等，回肠密度最低；５ Ｈ Ｔ 免疫反应阳性细胞多分布在肠腺处，绒毛中较少。该细胞形态多样，大多为锥形、梭形、椭圆形或圆形。有的细胞基底部有突起，后者可伸向腺腔或肠腔，其末端含有５ Ｈ Ｔ 阳性物质，提示此种细胞有旁分泌和外分泌功能     

体视学基本知识

本文对体视学方法用于形态定量研究的基本步骤,注意事项,所需的基本工具和所涉及的基本理论与抽样方法等进行了介绍。     

DataVoice: A microcomputer-based general purpose voice-controlled data-collection system

An integrated system of hardware and software has been developed to combine the input of coordinate data from a digitizing pad with voice input for object identification or classification. The menu and voice-controlled software generates a sequential ASCII file which contains an object identification section and a data section. Rules for data entry and analysis programs have been developed for several applications. These are being tested and include general stereological analyses, bone, kidney and skin histomorphometry, boundary analyses, neuron classification and malignancy grading.     

普鲁卡因胺对血小板及其开放管道系统形态结构改变的影响

用比浊法、电镜观察法和体视学计量法研究普鲁卡因胺对血小板聚集、形态改变和开放管道系统改变的影响。结果：普鲁卡因胺明显地抑制血小板聚集反应，抑制率为７０．６％～９１．２％；抑制血小板形成伪足和粘附，抑制开放管道系统形态结构参数值的改变：体视计量学的各种形态学参数值随药物浓度增加而逐渐接近空白对照组的水平，但与生理盐水对照组比较有显著差异，说明ＰＡ显著地抑制血小板及其开放管道系统形态结构的改变。     

大肠癌不同浸润深度和转移淋巴结中癌细胞体视学参数测定及其意义

目的对比分析89例不同浸润深度和转移淋巴结中大肠癌细胞体视学参数,从体视学角度探讨大肠癌浸润与转移的关系.方法根据体视学原理,测定大肠癌组织不同浸润深度及转移淋巴结中癌细胞的体视学参数,依据有关体视学公式计算出参数Vvn、Svn、λ、v、Nv、Kv、k、s,比较这些参数在不同组织间的差异.结果参数Vvn、Svn、v、s的大小是依浅层癌组织到深层癌组织的顺序增加,λ、Nv、k的参数大小则依浅层癌组织到深层癌组织的顺序递减,说明癌细胞浸润肠壁越深,其分化越低,其中Vvn、Svn参数有显著性差异;浅层癌组织和淋巴结转移癌组织所测上述体视学参数结果显示,两者无明显差异.结论癌组织浸润肠壁越深,其癌细胞的分化越低;淋巴结转移的癌组织可能来源于浅层癌组织.     

Prenatal exposure to diclofenac sodium changes the morphology of the male rat cervical spinal cord: a stereological and histopathological study

Diclofenac sodium is one of the most commonly used non-steroidal anti-inflammatory drugs. It may cause alteration in the nervous system during neuronal development. However, there is no investigation concerning its role in the cervical spinal cord. Pregnant rats were divided into two groups, namely drug-treated and control (saline-injected) groups. To obtain the offspring of the drug-treated group, a dose of 1 mg/kg daily diclofenac sodium (Voltaren, 75 mg/3 ml ampoule, Novartis) was injected into the pregnant rats beginning from the 5th day after mating to the 20th day of the pregnancy. To obtain the control group of offspring, serum physiological at a 1 ml/kg daily dose was injected into the pregnant control rats during the same period. Male offspring were obtained after delivery and each group was divided into two subgroups: 4-week-old and 20-week-old. The total neuron number in diclofenac sodium-treated rats was significantly lower than in the control group animals. The total volume of the cervical spinal cord segments (C1-C4) was also estimated. There was a significant difference between the volumes of the two groups, especially in the 20-week-old subgroup. This may suggest that development of neurons and volume of cervical spinal cord are affected in prenatal animals after administration of diclofenac sodium.     

电针对先天性脑损伤仔鼠脑白质神经髓鞘修复的影响

目的观察和探讨电针对宫内感染致脑损伤仔鼠脑白质神经髓鞘修复的影响和机制。方法连续两天对孕第17天的孕鼠腹腔注射脂多糖（LPS）[450μg/（kg.d）],制备先天性脑损伤仔鼠模型;对照组孕鼠腹腔注射等量无菌生理盐水,随机选取对照组仔鼠22只;随机选取LPS组仔鼠40只,随机分为非干预组22只和电针组18只,电针组电针组于第7天开始进行干预,分别于第14天、第28天用电子显微镜观察各组仔鼠脑白质神经髓鞘,并应用体视学技术测量和计算出脑白质神经髓鞘厚度平均值。结果非干预组、电针组仔鼠脑白质神经髓鞘厚度平均值均明显低于对照组（P﹤0.01）,电针组仔鼠脑白质神经髓鞘厚度平均值高于非干预组（P﹤0.01）。结论电针干预可促进脑损伤仔鼠脑白质神经髓鞘修复。