以主药溶解性能筛选体外经皮渗透试验中的接收液

目的:筛选舒心贴剂中CVD体外经皮渗透试验的接收液.方法:采用异氰酸苯酯柱前衍生化RP-HPLC法,测定CVD在不同接收液中的饱和浓度,根据溶解性能筛选体外经皮渗透接收液.结果:七种接收液中以30%乙醇/生理盐水中CVD的浓度最高.结论:以主药溶解性能筛选体外经皮渗透试验的接收液,方法可靠、简便.     

前交叉韧带重建术后短期内移植体黏性变化

目的 研究前交叉韧带重建(anterior cruciate ligament reconstruction, ACLR)术后短时间内移植体黏性的变化规律。方法 选择6只雄性新西兰兔,以跟腱为移植体制作单侧膝关节ACLR动物模型。ACLR术后15 d将实验兔安乐死,取出移植体、健康的前交叉韧带(anterior cruciate ligament, ACL)和跟腱。测量移植体的截面积,分别进行0.1、1 MPa平衡条件的蠕变实验,并计算黏性系数,总结移植体的黏性变化规律,与健康ACL进行对比。结果 移植体的截面积在手术后15 d内缓慢上升。ACL与移植体的黏性呈非线性变化。在不同应力下,黏性系数有较大差异。移植体黏性系数随ACLR术后时间呈下降趋势,但在低应力下更明显。结论 本文建议,ACLR术后早期康复过程中,应使用助行器,降低步频,并避免踢、踹等对关节冲击的运动。     

Fibrin glue assisted amniotic membrane graft reconstruction of conjunctival fornix and canthus following excision of conjunctival tumours

Conjunctival tumors involving non-limbal locations, such as the fornix and canthus, are typically excised using a “non-touch” technique, often with a wide surgical margin. Reconstruction of these large defects can be difficult due to the contour of the ocular surface and are often complicated by shortening of the fornix, symblepharon formation, and restriction of eye movements. In our experience, the use of amniotic membrane grafts combined with the sealant properties of fibrin glue such as Tisseel® has improved our surgical outcomes during the reconstruction phase. We would like to highlight and describe our surgical technique using fibrin glue and squint hooks to aid amniotic membrane graft reconstruction for surgically challenging locations in the fornix and canthus following excision of conjunctival lesions, with excellent surgical outcomes.     

脊髓纵裂的手术疗效观察

收治脊髓纵裂４６例，其中２９例作神经功能状态的临床评价及胫后神经皮层体感诱发电位检查。结果：非手术组随访后神经功能缺陷无改善（Ｐ＞０．０５）；２０例手术组神经功能的临床评分明显增加及胫后神经皮层体感诱发电位明显改善（Ｐ＜０．０５）。表明手术不仅可阻止神经功能缺陷的发展，而且可使其得到改善，是一有效的治疗手段。     

Use of Gamma-Irradiated Cornea to Plug Sclerostomy Site During Tube Shunt Revision

Tube shunt implantation is a common procedure for control of intraocular pressure (IOP). However, tube revision and repositioning must sometimes be performed, and this involves removing the tube from its sclerostomy site. This site is prone to leaking and this may cause postoperative hypotony. We describe a novel and cosmetically acceptable technique of plugging and covering the sclerostomy site with gamma-irradiated corneal tissue.     

大鼠坐骨神经缺损半个月后人工组织神经移植物修复的实验研究

为了观察人工组织神经移植物对大鼠坐骨神经非新鲜损伤的修复作用。我们在成年SD大鼠左侧股中部切除部分坐骨神经制造神经缺损模型。15d后,实验组(9只)用人工组织神经移植物修复神经缺损,自体神经修复作为阳性对照(6只),保持神经缺损为阴性对照(6只)。第二次手术后3个月,电生理学、形态学结果显示实验组的再生神经虽略差于自体对照组,但明显优于缺损对照组,腓肠肌的萎缩形态学指标变化则较接近自体对照组。实验结果表明人工组织神经移植物对已缺损15d的大鼠周围神经具有较好修复作用。     

皮肤老化的几种常见细胞和富血小板血浆治疗进展

皮肤抗老化领域的细胞治疗技术自问世以来,经历多次更新和优化,目前临床上公认安全性和效果较好的细胞治疗制剂包括脂肪干细胞及其培养基提取物、成纤维细胞、皮肤间充质干细胞以及富血小板浓缩物、富血小板血浆制剂等.然而,针对这些制剂的作用机制及优缺点还缺乏系统的总结,本文将对此进行详细阐述.     

尘螨变应原皮肤点刺试验与血清特异性IgE的相关性研究

目的 探讨尘螨变应原皮肤点刺试验(skinprick test,SPT)结果与血清特异性IgE(specific IgE,S-IgE)检测值之间的相关性.方法 分析170例接受尘螨变应原SPT和相应的血清S-IgE检测的变应性鼻炎患者不同皮肤风团直径和不同皮肤反应指数(skin index,SI)对应的血清S-IgE的差异.结果尘螨变应原SPT不同皮肤风团直径和不同SI对应的血清S-IgE存在显著性差异,随着皮肤风团直径或SI的增加,血清S-IgE阳性率和级别也显著增加.结论尘螨变应原SPT结果和血清S-IgE结果存在相关性.     

眼睑皮肤缺损的三种基本皮瓣转移方式

介绍三种简单易行的皮瓣转移方式,可有效矫正眼睑皮肤缺损.     

Arthroscopy-assisted bone grafting for the treatment of SNAC stage I without radial styloidectomy

To report the clinical and radiological outcomes of arthroscopic bone grafting and percutaneous K-wire fixation without radial styloidectomy in patients with scaphoid nonunion advanced collapse (SNAC).We retrospectively analyzed the records of 15 patients with SNAC who were treated with arthroscopic bone grafting and percutaneous K-wire fixation and subsequently followed up for a minimum of 1 year between November 2009 and March 2018. The clinical outcomes were evaluated by comparing the range of motion (ROM), grip strength, the modified Mayo Wrist Score, and visual analog scale (VAS) scores for pain, all of which were measured preoperatively and at the last follow-up. The radiologic outcomes were evaluated by comparing the scapholunate (SL) and radiolunate (RL) angles preoperatively and at the last follow-up.All 15 cases of nonunion were resolved. The average radiologic union time was 9.7 ± 1.2 weeks. The average VAS score increased from 5.7 ± 2.3 (range, 2 − 10) preoperatively to 1.3 ± 1.3 (range, 0 − 3) at the last follow-up (P < .05). The average modified Mayo wrist score increased from 58.3 ± 14.0 preoperatively to 80.0 ± 9.2 at the last follow-up (P < .05). The mean ROM of the wrist improved, but there was no statistical significance. At the last follow-up, the mean flexion and radial deviation on the affected side were significantly decreased, and the mean extension on the affected side was significantly improved compared to the normal side (P < .05). The mean preoperative SL and RL angles were 66 ± 11.9° and 7.2 ± 6.8°, respectively, and were decreased to 50.4 ± 7.5° and 6.4 ± 5.2°, respectively, at the last follow-up. The mean SL angle was significantly corrected (P = .01).Arthroscopic bone grafting and percutaneous Kerschner (K)-wire fixation without radial styloidectomy are considered to be very effective methods for correcting scaphoid deformities to treat SNAC stage I. However, caution may be needed during the surgery to prevent reductions in flexion and the radial deviation of the wrist.