基于SPA技术的网络随访系统的设计与实现

目的：设计和实现具有随访数据录入/检索/导出、历史随访记录对比、疾病风险量表评估等功能的网络随访系统,使用户可以随时随地管理和分析患者随访数据。方法：基于目前较为流行的MEVN全栈开发架构,设计与实现了界面简洁、功能完善的随访系统SPA,并将其部署到云服务上。结果：成功地完成了基于SPA技术的网络随访系统,在临床科室取得了较好的应用,得到了医护人员的一致肯定。结论：采用MEVN架构的SPA技术具有开发效率高、适应复杂数据模型、并发性能好、易部署等特点。基于SPA技术研发的网络随访系统能有效提高随访效率,改善科研环境。     

悬吊式单切口腔镜手术治疗男性乳腺发育症

目的探讨悬吊法单切口腔镜手术治疗男性乳腺发育症的临床价值。方法 2007年12月～2009年6月对11例男性乳腺发育症采用悬吊法行皮下单纯乳腺切除术,在隐蔽部位做2cm小切口,利用SPA器械完成手术。结果 11例手术均获成功,手术时间105～145min,平均122min。术后住院3～10d,平均5.3d。11例随访2～20个月,平均12个月,外形均较满意,乳头形态正常。结论单切口悬吊法腔镜下乳腺切除术有较好的美容效果且手术安全。     

Initial Surgeon Training for Single Port Access Surgery: Our First Year Experience

Background:

We have developed a single port access (SPA) surgical technique that allows for procedures to be done through a single umbilical port incision <20 mm in length. For a new approach to be universally beneficial, it needs to be easily learned and applied.

Methods:

Single port access abdominal procedures are performed through one umbilical incision where skin and soft tissue flaps are raised from the underlying fascia to allow insertion of up to 4 instruments. Fifty surgeons with varying degrees of laparoscopic training participated in SPA training programs at Drexel University to learn and apply the SPA technique through participation in an animate (porcine) laboratory.

Results:

All surgeons successfully performed the SPA access technique without difficulty and completed the cholecystectomy in <55 minutes (average, 42). Eight surgeons successfully performed placement of a cholangiogram catheter. All recognized the value of a formal training symposium to learn SPA techniques before performing SPA procedures in their practice.

Conclusions:

The SPA technique has been successfully shown to be an approach that is easily learned and accomplished. We believe this is a necessary and important bridge towards proficiency in performing SPA procedures in clinical patients.     

快速ELISA法诊断血吸虫病的应用研究

目的对快速酶联免疫吸附试验（ELISA）的血吸虫病诊断实验条件进行优化,并观察其应用价值。方法同时将抗原、待测血清、HRP-SPA共置进行反应,替代常规ELISA法的分步反应。通过比较不同稀释度HRP-SPA的检测效果,确定其最佳工作浓度。观察待测血清和HRP-SPA不同混合反应时间对检测效果的影响,优化快速ELISA法的检测条件。用快速ELISA法、常规ELISA法和胶体染料试纸条法（DDIA）平行检测慢性血吸虫病人、华支睾吸虫病人及健康人血清,观察快速ELISA法的血吸虫病诊断实用价值。结果 HRP-SPA的最佳工作浓度为1∶2 000,待测血清和HRP-SPA最佳混合反应条件是37℃同步反应60 min。快速ELISA法检测慢性血吸虫病人血清抗体阳性检出率为93.8%,与华支睾吸虫病人血清的交叉反应率为4.0%,检测健康人血清的假阳性率为1.5%,与常规ELISA法和DDIA检测结果相似。结论快速ELISA法具有快速、简便等优点,是一种简便实用的血吸虫病免疫诊断方法。     

联系数学中联系度的表达及其在卫生装备评估方面的应用

介绍了一种处理模糊不确定问题的新方法——集对分析原理，并总结了几种联系度的几种计算方法，可为多目标决策中不确定性的描述与表达提供解决的思路，并应用卫生装备评估实例进行了不确定性的研究，建立了综合评估的数学模型，验证了多种联系度计算方法的实用性、有效性。     

ABC-ELISA法诊断肠螨病的研究

目的　探讨生物素 -亲和素酶联免疫吸附试验 (ABC -ELISA)在肠螨病诊断中的应用价值。方法　采集 4 8例肠螨病患者血清 ,用ABC -ELISA法检测血清中螨特异性抗体IgG ,并与葡萄球菌A -蛋白酶联免疫吸附试验 (SPA -ELISA)进行比较。结果　用ABC -ELISA法和SPA -ELISA法检测 4 8例肠螨病患者血清中的螨特异性抗体IgG阳性率分别为89 5 8% (43/ 4 8)和 5 6 2 5 % (2 7/ 4 8) ,两者比较 ,差异具显著性 (χ2 =13 5 0 ,P <0 0 1)。结论　ABC -ELISA法是实验室诊断肠螨病的一种有效方法     

单光子跟骨骨密度仪的研制

介绍一种基于SPA测量方法的跟骨骨密度仪的设计原理,包括机械系统和控制系统的设计及工作原理。还介绍了该仪器达到的主要精度及产生的积极效果。     

Scintillation proximity assay in lead discovery

Background: Scintillation proximity assay (SPA) is a homogeneous scintillant bead-based platform for the measurement of biological processes and plays an important role in the identification of active chemical entities in drug discovery. Objective: The design and development of solid-phase SPA approaches are examined and compared with alternative non-radiometric fluorescence-based technologies. Methods: This review provides background on the principle of SPA and its application to biomolecular interactions from a variety of biological sources. Conclusion: The SPA approach is well suited to the demands of commercial high volume automation and assay miniaturization for target-based high-throughput screening campaigns on synthetic and natural product libraries as well as for benchtop characterization and confirmation studies. In the near future, innovations in the way SPA and fluorescence-based screening strategies are multiplexed will improve our comprehensive understanding of cellular system biology and dramatically advance the lead discovery process for the treatment of complex target-related disorders.     

Detection of specific IgM of Cox B virus in patients with acute myocardial infarction

<正> In this paper, the authors determined the specific antibody IgM of Cox B virus in serum of patients with acute myocardial infarction, using SPA absorption, combined with immunoenzyme staining method. Meanwhile, the antibodies were determined with mieromethod of neutralization test.The results showed that in 26 patients with acute myocardial infarction, the specific antibody IgM ratio of Cox B virus was 57.6%, and the double blood tests also showed that patients with acute myocardial infarction was really suffering from the viral infection.