Establishment of the culture technique of pulmonary vascular pericytes and its identification in rats

Summary In order to study the cellular origin of muscularization in non-muscular arterioles of the lung, the pulmonary vascular pericytes-culture was established. The terminal lung tissue of the rat was taken out and minced. Then 0.5 % of type IV collagenase solution was added for digestion and the microvascular segments were obtained by screening. The targeted cells were cultured by “selective conditioned media”. Under phase-contrast microscope, the cultured cells were large in size with ragged margin and numerous pseudopodia, which imparted tubule-like structure. There was no contact inhibition in growing cells, so multiple layers developed. When they were confluent, there were morphologically no “hillock and dale” growth pattern as in smooth muscle cells or “weave-like” pattern as in fibroblasts. The ultrastructure of cultured cells showed numerous digital processes, moderate amount of rough and smooth endoplasmic reticulum, rich Golgi’s apparatus, microfilaments, few lysosomes without myofilaments and dense bodies. Immunohistochemical staining revealed that the cultured pericytes had same kind of cellular skeletal protein, α-SM-actin, like smooth muscle cells. The cultured cells also exhibited positive reaction to CD₃₄ antigen and S-100 antigen, which were negative in smooth muscle cells and fibroblasts. The cell growth pattern, ultrastructure and immunological phenotype suggested that the cultured cells had characteristics of vascular pericytes. Pericytes are one of the components of microvascular cells, and the establishment ofin vitro culture technique of pericytes is of significance for further exploration of the muscularization of non-muscular arterioles in lung and the mechanism of structural remodeling of pulmonary vessels. This project was supported by the grant of National Nature Sciences Foundation of China (No. 39570289).     

视力客观检测方法的研究

目的研究用视觉诱发电位（ｖｉｓｕａｌｅｖｏｋｅｄｐｏｔｅｎｔｉａｌ,ＶＥＰ）作为视力客观检测的可行性。方法对４０只视力不等的患眼和５０只正常眼,分别记录用不同大小方格（８°、４°、２°、１°、３０、１５和６）刺激时的ＶＥＰ波形。结果患眼组和正常组Ｐ１００振幅及潜时在统计学上有显著性差异。视力与能诱发出波形的最小方格间有明显关系：最小方格为６、１５、３０和１°,平均视力分别为１．１３,０．６２,０．１８和０．０７。视力与Ｐ１００振幅呈正相关,与潜时呈负相关。结论用诱发出ＶＥＰ波形的最小方格大小可估计患者的视力范围     

中药厚朴及其类似品的有效成分分析

本文对中药厚朴及其类似品中的有效成分——厚朴酚、和厚朴酚及木兰箭毒碱分别进行了高效液相色谱法和双波长扫描法测定;对其挥发油中化学成分进行了气-质联用分离与鉴定。     

Human acoustic neurinomas: Nervous system specific biochemical parameters

Summary A series of 24 human acoustic neurinomas from 24 patients has been assayed for several biochemical parameters characteristic of the nervous system. S 100 protein, 2, 3-cyclic nucleotide 3-phosphohydrolase activity, and the myelin lipids galactosylceramide and sulfogalactosylceramide (sulfatide). Myelin basic protein was not detected. These findings further support the neuroectodermal origin of the human acoustic neurinoma, and provide additional biochemical markers for further study.     

pD2-, pA2- and pD2'-values of a series of compounds in a histaminic and a cholinergic system.

(1) Affinity and intrinsic activity values of 75 compounds for a histaminergic and a cholinergic system are presented. The quantitative correlations between the affinity values of 35 compounds and some physicochemical constants (Van der Waals volume, lipophilicity, number of hydrogen atoms on the protonated amine) are discussed. (2) Absence of systematic differences between pD2 and pA2 of partial agonists supports the assumption that these values are equivalent expressions of the same affinity. (3) The "mimetic moiety" in a number of the antihistaminic test compounds hardly contributes to their affinity. The affinity mainly depends on an interaction tendency with additional receptor areas. (4) The correlation between pA2 and pD2' of the whole series of compounds in the histaminergic system is artificial. The method only allows determination of both values if their ratio lies between certain limits. (5) The correlation between pA2 and pD2' for 16 closely related compounds in the guinea pig ileum and for nearly all compounds in the rat intestine has to be explained by an influence of the structural differences on drug transference and/or the less specific binding forces. (6) The metactoid receptors in the two systems are different structures. (7) Possible molecular modifications to maximize the separation of antihistaminic form cholinergic affinity are suggested.     

雷公藤内酯醇抑制大鼠脑局灶性缺血再灌注后脑组织IL-1βmRNA表达的研究

目的　观察雷公藤内酯醇 (Triptolide ,TL)对局灶性脑缺血再灌注大鼠脑组织内IL 1βmRNA水平变化的影响 ,探讨雷公藤内酯醇对大鼠局灶性缺血再灌注脑损伤保护作用的机制。方法　建立大鼠局灶性脑缺血再灌注模型 ,观察应用雷公藤内酯醇 (0 2和 0 4mg·kg-1)对大鼠脑组织微血管内附壁中性粒细胞计数 ,以及神经功能受损程度的影响。应用RT PCR技术 ,检测大中动脉闭塞侧脑组织IL 1βmRNA表达水平的变化情况。结果　与对照组比较 ,雷公藤内酯醇两治疗组大脑中动脉闭塞侧微血管内附壁中性粒细胞计数、脑组织IL 1βmRNA水平均明显下降。神经功能受损程度也明显改善。结论　雷公藤内酯醇具有明显抑制缺血再灌注大鼠脑组织内IL 1βmRNA表达、降低IL 1β含量和抑制白细胞浸润的作用。这可能是雷公藤内酯醇对大鼠缺血再灌注脑损伤保护作用的机制之一     

β-细辛醚在大鼠体内的药代动力学

目的了解石菖蒲主要成分β-细辛醚的药代动力学特征。方法以HPLC法测定ig大鼠血清和各器官（脑、心、肺、肾、肝）中β-细辛醚的浓度，把器官当作相对独立的系统，用DAS软件进行药代动力学分析。结果β-细辛醚在大鼠体内的药代动力学过程属一级一室动力学模型，血清半衰期为54 min；达峰时间12 min；达峰浓度3.19 mg·L^-1。各器官与血清有相似的动力学过程。结论β-细辛醚在体内吸收、分布、代谢较迅速，极易透过血脑屏障，大脑是重要的分布器官。     

Effect of Complexation with Hydroxylpropyl-β-Cyclodextrin on Solubility, Dissolution Rate and Chemical Stability of Prostaglandin E1

Aim To study the effect of complexation with hydroxylpmpyl-β-cycledextrin (HP-β-CD) on the solubility, dissolution rate and chemical stability of pmstaglandin E1 (PGE1), thereby providing a basis for preparing a stable solid or aqueous preparation of PGF1 formulated with HP-β-CD. Methods The effect of HP-β-CD on the solubility of PGF1 was studied by phase solubility method. The formation of inclusion complexes of PGE1 with HP-β-CD in the aqueous solution was confirmed by UV spectra, circular dichroism spectroscopy, and that in the solid state by IR spectra and X-ray diffractometry. An solid inclusion complex of PGF1 with HP-β-CD was prepared by lyophilization. The dissolution rate and stability of the inclusion complex were determined and compared with those of PGE1 alone. Meanwhile, the stability of PGF1 aqueous solutions in the presence of HP-β-CD was studied under different pH conditions. Results The solubility of PGF1 increased linearly with increasing HP-β-CD concentration in various pH buffered solutions, showing typical AL-type phase solubility diagrams. The stability and dissolution rate of the solid inclusion complex of PGE1 were significantly increased, compared with those of pure PGE1 . The stability of PGEI in HP-β-CD solutions was also obviously improved under acidic and basic conditions, but the stabilizing effect was absent under neutral conditions. Conclulsions The solubility, dissolution rate and chemical stability of PGE1 are markedly improved by complexation with HP-β-CD. It is quite possible to prepare a stable PGE1 inclusion complex-containing solid dosage forms, but almost impossible to obtain a stable aqueous preparation of PGE1 formulated with HP-β-CD.     

腹足纲Helix pomatia βC-血清蛋白的功能单位e中碳水化合物的定位和糖基化位置的鉴定

对腹足纲软体动物罗曼蜗牛Helix pomatia的氧运输蛋白(βc-血清蛋白)的功能单位e中的碳水化合物进行了研究。以该βc-血清蛋白为载体，通过限制性胰蛋白酶水解，产生片段a-c，ef，以及功能单位d，g，和h。以片段ef降解后的产物为研究对象，通过亲合色谱的分离纯化，得到功能单位e的一个馏分e2。该馏分经还原、吡啶乙基化和胰蛋白酶水解后，通过柱色谱的分离纯化，得到一个含有糖肽的成分。对该糖肽中糖组分和肽序列的分析，最终确定了功能单位e的两个糖基化位置：Asn-lll和Asn-387。通过与功能单位d和g的糖基化位置的比较得出结论：Asn-387为一恒定的糖基化位置。