间苯三酚联合米索前列醇在绝经后妇女取环中的应用

目的研究分析间苯三酚与米索前列醇联合用药在绝经后妇女取环患者的临床效果.方法选取我院于2011年3月至2012年3月收治的80例绝经后妇女取环患者的临床资料,将其按照随机分组的方式分为治疗组和对照组,治疗组42例患者采用药物联合治疗,即间苯三酚与米索前列醇治疗.对照组38例患者采用间苯三酚单独用药治疗,观察对比两组患者术中疼痛程度和宫颈松弛程度.结果两组患者术中疼痛程度,治疗组优于对照组,有统计学意义(P<0.05);两组患者宫颈松弛程度对比,治疗组软化有效率为97.62%,对照组软化有效率为78.95%,有统计学意义(P<0.05).结论绝经后妇女取环采用苯三酚与米索前列醇联合治疗,可以改善患者的临床症状且镇痛效果较好,安全有效,值得临床推广.     

术前口服止痛药配合人性化护理对商环包皮术患者疼痛的影响

目的：探讨术前口服止痛药配合人性化护理对商环包皮环切术患者术后疼痛的影响。方法：将2011年6月-2012年6月在本院进行商环包皮环切术的患者228例随机分为两组,实验组（114例）和对照组（114例）,对照组采用常规的术前护理,实验组采用术前121服止痛药并配合人性化护理,比较两组患者术后对疼痛的影响。结果：实验组术后24h内未出现剧烈疼痛患者,而对照组有31例出现剧烈疼痛甚至晕倒,两组比较,差异有统计学意义（P〈O．05）。结论：术前口服止痛药并实施人性化护理可有效降低商环包皮环切术患者术后的疼痛,提高患者围手术期的生活质量。     

绝经后妇女取环术中控制疼痛给药方法的效果评价

目的探讨绝绝经后妇女取环术中控制疼痛给药方法及效果。方法 120例绝经后妇女,平分3组,每组40例。一组：术前2d每晚口服米非司酮75mg,术前2h口服米索前列醇600μg。二组：术前2h经阴道后穹窿放置米索前列醇600μg。三组：利多卡因宫颈注射。结果三组扩宫效果比较,差异无统计学意义（P〉0.05）,但一组满意度略高于二、三两组,并且一组镇痛效果明显优于二、三两组差异有统计学意义（P〈0.05）。结论米非司酮配伍米索前列醇用于绝经后妇女取出IUD术前用药,对扩张宫颈和减轻疼痛效果最满意,具有很高的使用价值。     

Methylation of CpG sites in RNF180 DNA promoter prediction poor survival of gastric cancer

RNF 180, a novel tumor suppressor, has been implicated in the carcinogenesis and progress of gastric cancer. At present study, we found that lower expression of RNA180 was specific in gastric cancer tissues, and the inconsistently methylated levels of RNF180 promoter were identified in the gastric cancer tissues. Importantly, we demonstrated that the methylated CpG site count and four hypermethylated CpG sites (−116, −80, +97, and +102) were significantly associated with the survival of 400 gastric cancer patients, respectively. With multivariate survival analyses, we demonstrated that both the methylation of combined CpG (−116, −80, +97, and +102) sites and N stage were the independent indictor of prognosis of gastric cancer patients. Eventually, the methylation of combined CpG (-116, -80, +97, and +102) sites was identified to have smaller AIC value than N stage by mean of AIC calculation with the Cox proportional hazards model. These findings indicated that the quantitative detection of RNF180 promoter methylation had the intensive feasibility for evaluation the prognosis of gastric cancer patients in clinic.     

米非司酮联合米索前列醇在绝经后妇女取环术中的评价

目的:了解米非司酮联合米索前列醇提高绝经后妇女取环术中成功率。方法:选取我院接受取环术的绝经妇女在术前采用米非司酮,每片25毫克,取环前1周口服,每日50mg,共服用3次,空腹或餐后2小时服用。术前2小时口服0.4mg米索前列醇片。结果:患者手术时,宫颈较术前明显软化,除2例因宫颈内闭锁取环失败外,其余患者均成功取出宫内节育环,其中200例顺利取出,48例节育环部分嵌顿,将节育环剪断后抽丝取出,术中患者疼痛感较轻、出血量平均约6毫升,术后常规抗炎止血,术后一个月随访无异常。结论:术前应用米非司酮联合米索前列醇可有效软化、扩张宫颈,对患者术中的痛苦进一步降低,取得了取环成功率并具有较高的应用价值。     

带有ring(13)的B淋巴母细胞株JMSR13的建立

经微量全血EB病毒转化法建立了-Ring（13）染色体B淋巴母细胞株（JMSR13），该细胞呈悬浮生长，核型为2n=45，XY／46，XYring（13）。细胞群体倍增时间为54hr／代。Ring（13）染色体在复制时的交换次数是构成ring（13）染色体倍性改变的原因，Ring（13）衍生染色体的随机断裂和其在有丝分裂中的不分离是其产生遗传学效应的主要根源。     

宫内节育器取出失败后再次手术的探讨

目的 比较宫内节育器手术取出失败后再次手术的3种手术方式.方法 2005年1～12月在我院门诊取环失败入院患者92例,其中取器失败1次61例,2次26例,3次5例.随机分为官腔镜下引导取环组(30例)、中弯钳盲取组(32例)、B超引导下取环组(30例).结果 宫腔镜下引导取环组1例环异位子宫浆膜层下,行腹腔镜下取出;中弯钳盲取组2例未完整取出后行宫腔镜取出,2例异位子宫浆膜层下行腹腔镜下取出;B超引导下取环组1例环异位子宫浆膜层,1例嵌顿子宫肌层,极小部分穿出子宫肌层行腹腔镜取出,余均顺利取出.3组手术时间、出血量和手术费用比较有显著性差异(P＜0.05),3组术后感染率、患者接受情况比较无显著性差异(P＞0.05).结论 宫腔镜下引导取环组安全性高,但费用高;中弯钳盲取组有风险,但较经济;B超引导组介于两者之间.临床可根据具体情况选择手术方式.     

宫颈癌三维适形近距离治疗腔内联合插植施源器对比研究

目的：比较局部晚期宫颈癌三维适形近距离治疗Utrecht与Ring施源器剂量学差异。方法 MRI引导的三维适形近距离治疗局部晚期宫颈癌患者25例,间隔采用Utrecht与Ring施源器,共96次。按照施源器不同分成2个组,每组48次,其中26次先采用Ring施源器,22次先采用Utrecht施源器。考察高危CTV体积和在A点水平处宽、厚、D90;OAR的D2 cm3;V7 Gy 、W7 Gy,A、T7 Gy,A、W／T7 Gy,A;配对t检验差异。结果 Utrecht与Ring组高危CTV体积、在A点水平处宽、厚、D90均相近（P＝0．487、0．340、0．857、0．921）;膀胱、直肠、乙状结肠、小肠D2 cm3均相近（P＝0．136、0．802、0．985、0．458）;V7 Gy 、T7 Gy,A相近（P＝0．076、0．435）,W／T7 Gy,A为Utrecht组大于Ring组（P＝0．002）。结论高危CTV在A点水平处较宽,且在A点水平处宽厚比大的患者,适合采用Utrecht施源器。     

Structure activity relationship studies of anti-inflammatory TMMC derivatives: 4-Dimethylamino group on the B ring responsible for lowering the potency

We previously synthesized 2',4',6'-tris(methoxymethoxy)chalcone (TMMC) derivatives with various substituents on the A ring that showed potent anti-inflammatory effects by inhibiting NO production in RAW 264.7 cells. The 2'-hydroxy group on the A ring could elevate the electrophilicity of Michael addition of GSH and electron donating groups on the A ring could stabilize the GSH adduct by decreasing the acidity of the alpha-hydrogen. Using this interpretation, we tested various substituents on the B ring and established a proper balance between biological activity and the position of the electron donating or electron withdrawing groups on the B ring. In this case, the 2'-hydroxy group was excluded because it could cause the formation of GSSG through a phenoxy radical and can confuse the interpretation of the biological results. Chalcone derivatives without 2'-hydroxy are likely to deplete cellular GSH levels by a Michael addition process. Strong electron donating groups on the B ring, such as 4-dimethylamino group, gave the weakest inhibition of NO production. A 4-dimethyamino group on the B ring could decrease the stability of the GSH adduct by weakening the C-S bond strength through movement of an electron pair on nitrogen via an aromatic ring.     

Risk Factors for Pressure Sores in Hospitalized Acute Ischemic Stroke Patients

BackgroundAcute ischemic stroke patients are a group at high risk for pressure sores. It is important to identify risk factors for pressure sores in acute ischemic stroke patients in order to facilitate early adoption of appropriate preventive and treatment measures.MethodsData were derived from the China National Stroke Registry. Acute ischemic stroke patients aged >18 years who presented at the hospital within 14 days after the onset of symptoms were eligible for this study. Comprehensive baseline data were collected. The definition of pressure sores was based on assessment at discharge of whether the patient had pressure sores at any time during hospitalization.Results12,415 patients with a mean age of 67 years and a mean length of hospitalization of 14 days were included in the study. Among these patients, 97 (0.8%) had pressure sores during hospitalization. In the multivariate analysis of risk factors for pressure sores, age (each increment of 5 years), being unmarried, NIHSS at admission (each increment of 3 points), mRS at admission (3-5 points), diabetes mellitus, hemoglobin at admission (each incremental reduction of 10 units), and history of peripheral vascular disease all were significantly correlated with the occurrence of pressure sores among acute ischemic stroke patients during hospitalization.ConclusionsOld age, severe neurological disability, being unmarried, low hemoglobin, and history of diabetes mellitus and peripheral vascular disease were risk factors for pressure sores in acute ischemic stroke patients.