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41.
José Henrique Furtado Campos Francisco Assis Lima Costa 《Revista do Instituto de Medicina Tropical de S?o Paulo》2014,56(4):297-300
In this study, we detected Leishmania spp. infection in
R. sanguineus collected from dogs that were naturally infected
with L. (L.) infantum. We examined 35 dogs of both sexes and unknown
ages. The infected dogs were serologically positive by the immunofluorescence
antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Quick Test-DPP
(Dual Path Platform), as well as parasitological examination of a positive skin
biopsy or sternal bone marrow aspiration. Ten negative dogs were included as
controls. The ticks that infested these dogs were collected in pools of 10 adult
females per animal. The PCR was performed with specific primers for
Leishmania spp., which amplified a 720-bp fragment. Of the 35
analyzed samples, a product was observed in eight samples (8/35; 22.9%). We conclude
that the presence of parasite DNA suggests that ticks participate in the zoonotic
cycle of canine visceral leishmaniasis, in the city of Teresina, Piauí. 相似文献
42.
Yousr Galaï Mario Canales Mourad Ben Saïd Mohamed Gharbi Moez Mhadhbi Mohamed Jedidi José de La Fuente Mohamed-Aziz Darghouth 《Vaccine》2012
The Rhipicephalus microplus recombinant Bm86-based tick vaccines have shown their efficacy for the control of several Hyalomma cattle ticks genera, namely H. dromedarii and H. anatolicum. However, H. scupense species, the most important tick in North Africa has never been studied. Vaccination trials using either a recombinant Bm86-based vaccine or a recombinant Hd86-based vaccine (the Bm86 ortholog in H. scupense) were conducted in cattle against immature and adult H. scupense ticks and adult H. excavatum ticks. The results showed a 59.19% reduction in the number of scupense nymphs engorging on Hd86 vaccinated cattle. However, cattle vaccination with Bm86 or Hd86 did not have an effect on H. scupense or H. excavatum adult ticks infestations. These results showed that Hd86 vaccines are selectively effective against H. scupense immature instars and emphasize on an integrated anti-tick vaccine control in North Africa. 相似文献
43.
目的观察中药百部、枫树果对微小牛蜱的防制作用。方法湘南林区9个采样点对带有微小牛蜱的耕牛12头随机分为2组,分别用百部、枫树果乙醇提取液;1%的敌百虫溶液观察比较其对微小牛蜱的击倒作用以及对微小牛蜱的中枢神经团、生殖系统的影响。结果中药组较敌百虫组对微小牛蜱中枢神经团、雄蜱生殖孔有明显的损害作用(P<0.01),中草药组和敌百虫组对微小牛蜱的击倒作用两组比较差异无显著性(P>0.05)。结论中药组和敌百虫组对微小牛蜱均有明显的击倒作用,但对神经系统、生殖系统的影响前者明显优于后者,中草药且有对人畜几乎无毒等优点。 相似文献
44.
45.
Peripheral cellular and humoral responses to infestation with the cattle tick Rhipicephalus microplus in Santa Gertrudis cattle
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E. K. Piper N. N. Jonsson C. Gondro M. E. Vance A. Lew‐Tabor L. A. Jackson 《Parasite immunology》2017,39(1)
Resistance to cattle tick infestation in single‐host ticks is primarily manifested against the larval stage and results in the immature tick failing to attach successfully and obtain a meal. This study was conducted to identify immune responses that characterize the tick‐resistant phenotype in cattle. Thirty‐five tick‐naïve Santa Gertrudis heifers were used in this study, thirty of which were artificially infested for thirteen weeks with tick larvae while five animals remained at a tick‐free quarantine property to serve as a control group. Following thirteen weeks of tick infestation, the animals in this trial exhibited highly divergent tick‐resistant phenotypes. Blood samples collected throughout the trial were used to measure peripheral immune parameters: haematology, the percentage of cellular subsets comprising the peripheral blood mononuclear cell (PBMC) population, tick‐specific IgG1 and IgG2 antibody titres, IgG1 avidity for tick antigens and the ability of PBMC to recognize and proliferate in response to stimulation with tick antigens in vitro. The tick‐susceptible cattle developed significantly higher tick‐specific IgG1 antibody titres compared to the tick‐resistant animals. These results suggest that the heightened antibody response either does not play a role in resistance or might contribute to increased susceptibility to infestation. 相似文献
46.
C. C. Constantinoiu A. Lew‐Tabor L. A. Jackson W. K. Jorgensen E. K. Piper D. G. Mayer L. Johnson B. Venus N. N. Jonsson 《Parasite immunology》2018,40(4)
This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance. Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick‐resistant phenotypes. There was a tendency for more CD3+, CD4+, CD8+, CD25+, γδ T cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T cells and CD4+ cells. Most of the cattle developed intra‐epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies (mAbs) specific for CD45 and CD45 RO antigens reacted with skin leucocytes from a higher number of susceptible cattle than resistant cattle. Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mAbs specific for CD45 and CD45 RO directly, or a test for CD45 genotype might be developed as markers of tick susceptibility or resistance. 相似文献
47.
48.
四川东部南川县是莱姆病流行区。本文对300例居民进行了血清学调查,抗莱姆病螺旋体IgG抗体阳性54人,人群自然感染率为18%。35例经临床和血清学确诊的莱姆病患者,其临床表现为慢性游走性红斑7例,关节炎13例,面神经麻痹7例,多发性神经炎7例,脑膜炎2例和心脏异常1例。47只微小牛蜱的中肠标本,有3只检出了螺旋体,带菌率为6.4%。 相似文献
49.
Renard G Lara FA de Cardoso FC Miguens FC Dansa-Petretski M Termignoni C Masuda A 《Insect molecular biology》2002,11(4):325-328
Efforts are being undertaken to control tick infestations that cause important economic losses. A cathepsin L-like endopeptidase of Boophilus microplus was expressed in Escherichia coli; the recombinant enzyme was capable of hydrolysing gelatin, tick vitellin and bovine haemoglobin. In this paper we focus on the expression and local of synthesis of this enzyme in the tick. RT-PCR experiments showed that this endopeptidase is transcribed in the gut of partially engorged tick females. In immunoblotting, polyclonal antibodies against the recombinant enzyme reacted with proteins of larvae older than 5 days, of fully and partially engorged female gut. In immunolocalization experiments the enzyme was localized in probable secretory cells of the gut. Based on our findings we postulate that BmCL1 may be involved in haemoglobin degradation in the B. microplus gut. This enzyme may be used as target for the control of this parasite. 相似文献
50.
Guerrero FD Bendele KG Chen AC Li AY Miller RJ Pleasance E Varhol R Rousseau ME Nene VM 《Insect molecular biology》2007,16(1):49-60
Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial analysis of gene expression (SAGE) was used to analyse differential gene expression in response to OP treatment and to compare the responses of OP-treated and -untreated resistant and susceptible tick larvae. An R. microplus Gene Index was used as an EST database to identify genes which corresponded to SAGE tags whose abundance changed in response to acaricide exposure. Relative quantitative RT-PCR was used to confirm the differential expression results from the SAGE experiments. Of particular interest is a SAGE tag which corresponds to a cytochrome P450-like EST in the Gene Index which was more abundant in untreated OP resistant larvae compared to untreated OP susceptible larvae. This SAGE tag was also more abundant in OP resistant larvae treated with OP compared to OP susceptible larvae treated with OP. 相似文献