蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其机制

目的 探讨蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其抗肿瘤机制.方法 四甲基偶氮唑蓝(MTT)比色法检测蝎毒重组蛋白对人肺癌A549细胞增殖的抑制作用;荧光显微镜及流式细胞仪(FCM)技术观测蝎毒重组蛋白对肿瘤细胞内钙离子浓度([Ca2+]I)的影响,探讨[Ca2+]I变化时Ca2+的可能来源.结果 蝎毒重组蛋白对人肺癌A549细胞生长有明显抑制作用;荧光显微镜显示,蝎毒重组蛋白作用细胞48 h后细胞内钙离子荧光强度增强,提示[Ca2+]I升高;流式细胞仪显示蝎毒重组蛋白在不同情况下可显著增加肿瘤[Ca2+]I(P<0.05).结论 蝎毒重组蛋白对人肺腺癌A549细胞增殖具有显著抑制作用,可能与升高肿瘤[Ca2+]I继而诱导肿瘤细胞凋亡有关;其升高肿瘤[Ca2+]I是通过开放肿瘤细胞膜钙通道和肿瘤细胞内钙库释放两条途径实现.     

Granulocyte colony-stimulating factor treatment in AIDS patients

Summary Frequent complications of human immunodeficiency virus infection are hematopoietic failure and poor tolerance of myelosuppressive drugs. Reasons for neutropenia resulting from hematopoietic failure are infection of the bone marrow and hematotoxicity of treatment with zidovudine, ganciclovir, sulfonamides, and interferons. Moreover, tumor necrosis factor-, transforming growth factor- and interferon- have been shown to suppress proliferation of bone marrow cells. Both granulocyte (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) increase neutrophil counts and ameliorate phagocytic and bactericidic function of neutrophils. We report eight cases of AIDS patients with serious infections and neutropenia (< 750 cells/l), who were treated concomitantly with recombinant human G-CSF (3–4 g subcutaneously per kilogram body weight daily). G-CSF treatment was well tolerated in all patients and showed no side effects or disturbances of other lineages than neutrophils. Life-threatening bacterial infections were treated successfully by stimulating the neutrophil immune system. This therapy shortened the duration of subsequent treatment with antibiotics. Since human immunodeficiency virus infects CD4-positive monocytes and macrophages, which are stimulated by GM-CSF, G-CSF seems to be the cytokine of choice, if stimulation of the neutrophil lineage is warranted.Abbreviations G-CSF granulocyte colony-stimulating factor - GM-CSF granulocyte-macrophage colony-stimulating factor - HIV human immunodeficiency virus     

Liver metastasis from rectal cancer with prominent intrabile duct growth

Intrabiliary growth of liver metastases from colorectal cancer has rarely been studied. A surgically resected case of a metastatic liver tumor with prominent intrabiliary growth derived from rectal cancer is reported. The patient was a 62-year-old man who had received a low anterior resection for rectal cancer in March 2000. He was re-admitted due to obstructive jaundice in January 2003, and was diagnosed with hepatic malignancy in segment II of the liver with an intrabiliary tumor extending from the intrahepatic bile duct of segment II to the common hepatic duct. He underwent a left hepatectomy, a partial resection of segment VI, and an extrahepatic bile duct resection with reconstruction of the biliary tract. In the resected specimen, there were whitish tumors of 3 cm and 1.5 cm in diameter in segments II and VI, respectively, and an intrabiliary tumor originating from the main tumor in segment II extended to the common hepatic duct. Both the liver tumors and the intrabiliary tumor consisted of a well- to moderately differentiated adenocarcinoma, which showed the same histological features as the rectal cancer. The immunohistochemical findings strongly supported that these tumors, including the intrabiliary growth, were liver metastasis from the rectal cancer. The intrabiliary invasion and growth of metastatic liver tumors has generally been overlooked, notwithstanding their frequently observed biological behavior. The present case is informative, and further investigation into this type of metastatic liver tumor may be warranted.     

Value of serum and follicular fluid cytokine profile in the prediction of moderate to severe ovarian hyperstimulation syndrome

The aim of this study was to examine the role of serum and follicular fluid pro-inflammatory cytokines and vascular endothelial growth factor (VEGF) in the prediction of ovarian hyperstimulation syndrome (OHSS). A total of 156 consecutive women undergoing in-vitro fertilization were recruited. The study group comprised 12 women who subsequently developed moderate (n = 7) or severe (n = 5) OHSS. The two control groups were comprised of a randomized selection of 12 high-risk and 12 low-risk women in whom OHSS did not develop. Serum was collected on days of human chorionic gonadotrophin, oocyte retrieval, and embryo transfer. Serum and follicular fluid concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha (TNF-alpha), and VEGF were measured. Follicular fluid IL-6 concentrations at the time of oocyte retrieval and serum IL-8 concentrations at the time of embryo transfer were significantly higher in the OHSS compared to the two control groups (P = 0.026 and P = 0.017 respectively). Serum concentrations of TNF-alpha and VEGF showed no statistically significant difference between the OHSS group and the controls at any studied time point. This study suggests that follicular fluid IL-6 concentrations at the time of oocyte retrieval and serum IL-8 concentrations on the day of embryo transfer may serve as early predictors for this syndrome.     

蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其机制

目的 探讨蝎毒重组蛋白对人肺腺癌A549细胞的抑制作用及其抗肿瘤机制.方法 四甲基偶氮唑蓝(MTT)比色法检测蝎毒重组蛋白对人肺癌A549细胞增殖的抑制作用;荧光显微镜及流式细胞仪(FCM)技术观测蝎毒重组蛋白对肿瘤细胞内钙离子浓度([Ca2+]I)的影响,探讨[Ca2+]I变化时Ca2+的可能来源.结果 蝎毒重组蛋白对人肺癌A549细胞生长有明显抑制作用;荧光显微镜显示,蝎毒重组蛋白作用细胞48 h后细胞内钙离子荧光强度增强,提示[Ca2+]I升高;流式细胞仪显示蝎毒重组蛋白在不同情况下可显著增加肿瘤[Ca2+]I(P<0.05).结论 蝎毒重组蛋白对人肺腺癌A549细胞增殖具有显著抑制作用,可能与升高肿瘤[Ca2+]I继而诱导肿瘤细胞凋亡有关;其升高肿瘤[Ca2+]I是通过开放肿瘤细胞膜钙通道和肿瘤细胞内钙库释放两条途径实现.     

Role of oxidative stress,angiogenesis and chemo-attractant cytokines in the pathogenesis of ischaemic protection induced by remote ischaemic conditioning: Study of a human model of ischaemia-reperfusion induced vascular injury

Aims

We explored the effect of remote ischaemic conditioning (RIC) on endothelial function and on circulating mediators.

肿瘤坏死因子与肾小球系膜细胞的关系

目的研究肾小球系膜细胞的肿瘤坏死因子的自分泌功能,肿瘤坏死因子对肾小球系膜细胞作用的机制。方法利用体外培养的人和大鼠的肾小球系膜细胞,通过逆转录－多聚酶链反应、原位杂交和免疫组化方法,探讨它们之间的关系。结果肾小球系膜细胞既可产生肿瘤坏死因子,又有肿瘤坏死因子受体。结论肾小球系膜细胞是肿瘤坏死因子作用的靶细胞,肿瘤坏死因子通过旁分泌和自分泌两种途径作用于肾小球系膜细胞。