改良极化液对充血性心力衰竭QT离散度的影响

目的 :观察改良极化液治疗慢性充血性心力衰竭 (CHF)前后QTd、QTcd变化 ,探讨它对CHF近期预后影响。方法 :将80例住院CHF患者随机分为两组 ,A组 4 0例应用改良极化液 (葡萄糖 -胰岛素 -门冬氨酸钾镁液 )治疗 ,B组 4 0例仅静滴葡萄糖。结果 :两组患者RR间期在治疗前后比较有显著差异 (P <0 .0 1) ,A组治疗后QTd、QTcd与治疗前比较有明显缩短 (QTd分别为 90 .2± 4 0 .7与 5 0 .2± 30 .4 ,QTcd分别为 86 .4± 12 .4与 4 6 .2± 12 .4 ,P <0 .0 1) ,治疗后QTd、QTcd两组间比较有显著差异 (QTd为 5 0 .2± 30 .4与 78.9± 2 0 .2 ,QTcd为 4 6 .2± 12 .4与 70 .3± 16 .8,P <0 .0 1) ,两组间洋地黄过量中毒、恶性室性心率失常发生率比较 ,有显著性差异 (P <0 .0 5 )。结论 :改良极化液可明显缩短CHF患者QTd、QTcd ,减少洋地黄过量中毒、恶性室性心率失常发生 ,对CHF近期预后可能有改善作用     

Enantiomeric Resolution of Drug Compounds by Liquid Chromatography

Novel techniques have recently emerged to separate chiral drug compounds into pure enantiomers. The mechanism, experimental difficulties, and applicability of these methods can vary greatly, and the choices involved are not straightforward. The most significant new advances in the field of chiral separations have come from work done with liquid chromatographic systems and chiral stationary-phase columns. This review describes several commonly used approaches to chiral separation, diastereomeric derivatization, chiral mobile-phase additives, and three major types of chiral stationary phases. Although no single method can be judged superior for every drug application, it appears that chiral stationary phases have received the most attention recently and they are emphasized here.     

两种剂量氟碳部分液体通气治疗犬吸入伤的比较研究

目的：对比研究两种剂量氟碳（PFC）部分液体通气（PLV）对蒸气吸入性损伤犬的血气和血流动力学的影响，方法：将健康犬15条随机分面两组，即PFC6ml/kg组（PFC－6）和PFC 12ml/kg(PFC-12)组，采用蒸气吸入造成吸入性损伤模型后经气管内注入PFC实施部分液体通气（PLV）治疗，观察治疗后30min,60min,90min血气，血流动力学参数的变化，结果：PFC－6组PaO2在PLV治疗30min后明显升高（P＜0．05），在治疗60min,90min生的PaO2与致伤值比较无统计学意义，而PFC－12组的PaO2在PLV治疗后30min,60min,90min均明显升高（P＜0．05），与PFC－6组相比，PFC－12组的PaO2,PvO2均呈上升趋势，但未达显著水平（P＞0．05），在PLV治疗后各时间点的PaCO2HR,MAP,CVP 致伤值相比均无明显变化（P＞0．05），两组之间比较，各对应时间点的PaCO2,HR,MAP,CVP也均无明显变化（P＞0．05），结论：氟碳部分液体通气是一种新的通气技术，它能明显改善蒸气入伤犬的氧合作用，对血液动力学参数无不良影响，两种剂量氟碳部分液体通气治疗蒸气吸入性损伤未呈现明显剂量一效应关系。     

Changes in the content of Met-enkephalin in different brain structures during the development of immune response

Changes in the content of the opiate peptide Met-enkephalin at the early stages of immune response are studied in different structures of rats brain 20 min and 24 h after immunization with sheep erythrocytes. Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 123, No. 2, pp. 170–172, February, 1997     

血康口服液与他莫昔芬联合治疗免疫性血小板减少性紫癜疗效观察

联合应用血康口服液和他莫昔芬治疗成人免疫性血小板减少性紫癜(ITP)20例。疗程超过2月。15例患者的血小板(BPC)计数明显上升,有效率为75％(15/20),对于激素治疗无效者,仍有良效。对月经过多者和不适合应用激素者尤为适应。两药合用无明显副作用。治疗起效时间较快,平均13天。疗效持续时间较长,长者已达一年以上。     

Diving physiology and pathophysiology

Summary. Divers have worked at 500 m depth in the sea and have reached 700 m in simulated chamber dives. A prerequisite for this has been extensive physiological studies of the body's reactions to pressure and pressure changes. This paper reviews such physiological and pathophysiological studies with emphasis on recent developments.     

Indirect detection of anti-acetylcholinesterase compounds in microcolumn liquid chromatography using packed bed reactor with immobilized human red blood cell acetylcholinesterase and choline oxidase

The inhibiting compounds were separated by micro-column liquid chromatography in the mobile phase containing the natural substrate acetylcholine. A home-made packed bed microbioreactor system containing immobilized enzyme acetylcholinesterase (ACHE) in human red blood cell membrane and choline oxidase (CHO) from alcaligenes was used for the post-column conversion of acetylcholine to hydrogen peroxide which was detected by an electrochemical detector. The inhibition effect of the solutes caused a decrease in the acetylcholinesterase activity, a decrease in the formation of hydrogen peroxide and also a decrease in the response corresponding to the concentration of the solutes. The rate of the enzyme regeneration was also recorded. The micro-system was compared with a conventional LC system comprising commercially prepared enzyme reactor. The stability of the enzymes is at least 3 weeks at ambient temperature. The limit of detection depends on biological activity of inhibition and for galanthamine was 1 pmol.     

一种含胆甾醇介晶基团的侧链液晶高分子的合成

以２，４－二异氰酸甲苯酯（２，４—ＴＤＩ）、胆甾醇、丙烯酸β－羟丙酯为原料，合成了含胆甾醇介品基团的侧链液晶高分子、并用ＮＭＲ、ＩＲ和元素分析方法对其结构进行了鉴定。聚合物的相转变温度和形态织构用ＤＳＣ、Ｘ－射线衍射和偏光显微镜进行了测定和观察。     

Pharmacokinetics of intravenously administered sodium dichloroacetate in rabbits

ＰｈａｒｍａｃｏｋｉｎｅｔｉｃｓｏｆｉｎｔｒａｖｅｎｏｕｓｌｙａｄｍｉｎｉｓｔｅｒｅｄｓｏｄｉｕｍｄｉｃｈｌｏｒｏａｃｅｔａｔｅｉｎｒａｂｂｉｔｓＧｕＢｉｎ（顾斌）；ＳｏｎｇＬｉｎｇ（宋岭）；ＪｉａｎｇＹｏｎｇｐｅｉ（蒋永培）；ＷｅｎＡｉｄｏｎｇ（文...     

人体中氧氟沙星的立体选择性代谢

５名健康志愿者日服单剂３００ｍｇ氧氟沙星［（±）－Ｏｆ１］后，采用立体选择性的ＲＰ－ＨＰＬＣ手性流动相添加剂法测定尿中Ｓ－（－）－Ｏｆ１和Ｒ－（＋）－Ｏｆ１浓度结果显示Ｏｆ１在人体内呈现立体选择性代谢。尿中排泄的Ｓ－（－）－Ｏｆ１／Ｒ－（＋）－Ｏｆ１之比随时间变化。服药后２ｈＳ／Ｒ是０．８７５，２４ｈ增加到１．１５０．Ｓ－（－）－Ｏｆ１的消除半衰期（ｔ１／２）是４．５７ｈ；消除速率常数（ｋ）是０．１５４ｈ；药时曲线下的面积（ＡＵＣ）为１．１７ｍｇ·ｈ－１．ｍＬ－１；Ｒ－（＋）－Ｏｆ１的ｔ１／２为４．１８ｈ，ｋ为０．１６８ｈ－１，ＡＵＣ是１．７８ｍｇ·ｈ－１·ｍＬ－１；经配对ｔ检验这三对参数间有显著性差异，Ｐ值分别小于０．０１，０．００１和０．００５．因此两对映体在人体内呈现立体选择性处置