反相HPLC法同时测定饮料中的两种人造甜味剂

应用反相HPLC法，探讨了同一色谱条件下，食品中安赛蜜，糖精钠、咖啡因及苯甲酸的分离条件，并在此基础上建立了饮料中人造甜味剂安赛蜜、糖精钠的测定方法，取得了满意的结果。在反相C_(18)柱上，使用甲醇-冰乙酸-水（20+3.5+76.5）为流动相，可使安赛蜜、糖精钠、咖啡因，苯甲酸快速而良好地分离，254nm波长下检测，安赛蜜的线性范围为0.002～1.00g／L，最小检测量为0.04μg；糖精钠的线性范围为0.005～1.00g／L，最小检测量为0.08μg，在饮料分析中，外标单点校正法测得数据再用标准添加法处理进而求出的回收率分别为安赛蜜97.6％（n=5,CV=0.74%)、103.5%（n=5，CV=1.l%）、95.l%（n=5，CV=0.73%）；糖精钠97.9%（n=5，CV=0.66％）、99.9%（n=5，CV=0.73%)、102.1％（n=5，CV=0.18%）。本方法分析步骤简便，且检测波长适用于国产单波长检测器。     

24例骨折术后深部感染的原因及对策

目的 探讨骨折术后深部感染的原因、预防和治疗方法。方法 对骨折术后深部感染病灶行扩创后置管闭合灌洗。结果 24例经清创闭合灌洗平均27．5d后，感染均得以控制，效果满意。结论 骨折手术后深部感染原因是多方面的，宜积极预防，早期发现，及时扩创闭合灌洗，遗留肢体功能障碍者留待后期治疗。     

阿魏酸在血瘀证兔体内的药代动力学

为验证及研究症治药动学假说（ｓｙｎｄｒｏｍｅａｎｄＴｒｅａｔｍｅｎｔＰｈａｒｍａｃｏｋｉｎｅｔｉｃｓ，Ｓ＆ＴＰＫ）的客观性和基本规律，本文以ＲＰ－ＨＰＬＣ法同时测定了活血祛瘀药物阿魏酸在正常及高分子右旋糖酐所制血瘀症（微循环障碍）兔体内经时浓度，以ＭＣＰＫＰ药动学程序在ＣＯＭＰＡＱ８０３８６机上自动拟合了药动学参数。统计结果表明，与正常组相比，阿魏酸在血瘀症兔体内的分布容积（Ｖ＿１和Ｖ＿Ｂ）及消除速率（ＣＬＢ）显著减小（Ｐ＜０．０５），半衰期（ｔ＿（１／２β）及相同时间段曲线下面积（ＡＵＱ）非常显著增加（Ｐ＜０．０１）。结论：实验结果基本符合Ｓ＆ＴＰＫ的观点：即同一药物在不同证兔体内的药代动力学参数经统计学处理有显著差异。     

手性液晶的研究——Ⅸ．联苯羧酸酯类

合成了文题所述的十三个手性液晶化合物。经元素分析和红外光谱分析确证了其结构，并进行了相态、相变温度和电滞回线的测定，证实其为铁电液晶。     

Effects of granulocyte colony-stimulating factor on bacterial translocation due to burn wound sepsis

The presence of certain defects in both cellular and humoral immunity after thermal injury has been established. Likewise, the translocation of enteric bacteria to the mesenteric lymph nodes and to distant organs has also been observed following serious thermal injury. The effects of granulocyte colony-stimulating factor (G-CSF) on bacterial translocation, the small bowel mucosa, and cecal bacterial content were investigated in a rat model of burn wound sepsis in which albino Wistar rats were scalded over 30% of their bodies, after which the lesions were infected by 1×10⁸ colony-forming units (cfu)Pseudomonas aeruginosa. The control group was treated with 5% dextrose solution subcutaneously starting 2 days preburn, while the treatment group received 100μg/kg human G-CSF subcutaneously. On the 4th day post burn all animals were killed to examine the bowel and culture of the mesenteric lymph nodes (MLN), livers, and spleens. No significant differences were observed between the groups regarding the cecal bacterial content and small bowel; however, a difference was seen in the ratio of translocation in the MLN liver and spleen and quantitative MLN cultures. Based on these findings, G-CSF was thus found to be significantly effective in reducing bacterial translocation due to burn wound sepsis.     

FQ-I型清创(冲洗)机的研制

介绍一种电脑清创(冲洗)机的工作原理和系统功能。该机由水泵、水箱、压力调节器、温控器、喷枪等组成。操作时仅需打开开关，根据伤口类型调节压力、水温和冲洗水流的方式即可进行伤口冲洗，简单易行、省时省力，特别适合于战时救治。能降低火器伤的感染率、致残率，提高伤口清创冲洗的效率。     

复方桂枝汤中芍药甙煎出量的动态分析

 本实验运用CS－930双波长薄层色谱扫描仪，分析测定桂枝汤方剂在煎煮过程中，其主药芍药的主要化学成分芍药甙量的动态变化。结果表明，煎煮时间在30min时，芍药甙煎出量较高。该法简便、快速，色谱分离良好，排除了其它成分的干扰。平行性和重现性较好。     

火箭推进剂呼吸防护装备研究现状及发展趋势

本文对呼吸防护装备的应用、发展进行了综述，分析了火箭推进剂毒性对呼吸防护的要求和国内推进剂呼吸防护研究现状，对推进剂专用呼吸防护装备的发展趋势进行了探讨。     

S-nitrosoglutathione-containing hydrogel accelerates rat cutaneous wound repair

BACKGROUND: Nitric oxide (NO) plays a key role in wound repair and S-nitrosothiols like S-nitrosoglutathione (GSNO) are well known NO donors. METHODS: Animals were separated in two groups and submitted to excisional wounds on the dorsal surface at the first day. GSNO (100 microm)-containing hydrogels were topically applied on the wound bed in the GSNO group, daily, during the first 4 days. Control group was topically treated with hydrogel without GSNO for the same period. Wound contraction and re-epithelialization were measured. Animals were sacrificed 21 days after wounding. Samples of lesion and normal tissue were formalin-fixed, paraffin embedded for histological analysis. RESULTS: Wound contraction, measured 14 and 21 days after wounding, was greater in the GSNO group than in the control group (P<0.05 for both). The re-epithelialized wound area, measured 14 days after wounding, was higher in the GSNO group than in the control group (P<0.05). A higher amount of inflammatory cells was observed in superficial and deep areas of the granulation tissue of the control group compared to the GSNO group. Twenty-one days after wounding, thin red-yellow collagen fibers arranged perpendicularly to the surface were found in the granulation tissue of the control group, whereas in the GSNO-treated group collagen fibers were thicker and arranged parallel to the surface. Increased number of mast cells was observed in the GSNO group compared with that in the control group. Vascularization and myofibroblast distribution were similar in both groups. CONCLUSION: Topical application of GSNO-containing hydrogel during the early phases of rat cutaneous wound repair accelerates wound closure and re-epithelialization and affects granulation tissue organization.     

依西美坦片在不同介质中的溶出特性

目的:考察新药依西美坦片在3种不同介质中的溶出特性,以研究影响其口服吸收速率的主要因素和规律。方法:建立HPLC法检测其制剂含量和溶出液浓度,色谱柱:HypersilC18(150mm×4.6mm,5μm);流动相:甲醇:0.05mol/LKH2PO4溶液(60:40);检测波长:247nm。溶出介质选用水、0.1mol/L盐酸溶液及0.5%十二烷基硫酸钠溶液,采用转篮法,转速100r/min。结果:依西美坦片在水和0.1mol/L盐酸溶液中的溶出较差,在0.5%十二烷基硫酸钠溶液巾溶出迅速完全,60min时在3种介质中的溶出百分率分别为(60.25±3.76)%、(47.57±1.20)%和(82.24±0.96)%。结论:依西美坦片可能主要在肠道溶出,其溶出速率受介质影响大。