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1.
Deoxynivalenol (DON) has broad toxicity in animals and humans. In this study the impact of DON treatment on apoptotic pathways in PC12 cells was determined. The effects of DON were evaluated on (i) typical indicators of apoptosis, including cellular morphology, cell activity, lactate dehydrogenase (LDH) release, and apoptosis ratio in PC12 cells, and on (ii) the expression of key genes and proteins related to apoptosis, including Bcl-2, Bax, Bid, cytochrome C (Cyt C), apoptosis inducing factor (AIF), cleaved-Caspase9, and cleaved-Caspase3. DON treatment inhibited proliferation of PC12 cells, induced significant morphological changes and apoptosis, promoted the release of Cyt C and AIF from the mitochondria, and increased the activities of cleaved-Caspase9 and cleaved-Caspase3. Bcl-2 expression decreased with increasing DON concentrations, in contrast to Bax and Bid, which were increased with increasing DON concentration. These data demonstrate that DON induces apoptosis in PC12 cells through the mitochondrial apoptosis pathway. 相似文献
2.
Animal behaviour can be viewed as a stream of elements, which, once accurately described, can be counted and timed. Data acquisition techniques and tools are reviewed, and some strategies for collection and analysis of data using PC computers are suggested. Automated instruments are not satisfactory for the study of complex behaviour and as such systemic observation remains irreplaceable. IBM PC-type computers, with a wide range of analytical software (e.g., spreadsheets, statistical packages, technical graphics), are practical for data acquisition. Several systems which can satisfy different applications are reviewed. Some systems can communicate with a videorecorder, a facility which remarkably increases the accuracy of measurement; this is essential for meaningful analyses of the internal structure of behavioural streams (sequences, time patterns) or communication processes. The power of new tools enables behavioural measurement with the necessary complexity to allow a whole new set of questions to be addressed. However, it also increases demands for meaningful content and analysis of data. 相似文献
3.
Eileen J. Martin Kiran S. Panickar Michael A. King Malgorzata Deyrup Bruce E. Hunter Geehuan Wang Edwin M. Meyer 《Drug development research》1994,31(2):135-141
The potential cytoprotective actions of a novel nicotinic agent 2,4-dimethoxybenzilidene anabaseine (DMXB) were investigated in differentiated PC12 cells and transected rat septal cholinergic neurons in vivo. In NGF-differentiated PC12 cells, removal of both NGF and serum led to cell loss, a reduced % of cells expressing neurites, the release of lactate dehydrogenase, and a decrease in total cellular protein. Cell loss was apparent within 24 h, and remained constant between 4–8 days post-NGF removal. NGF alone (100 ng/ml), DMXB (10 μM), but not nicotine (10 μM), prevented these cell and neurite losses. DMXB-induced cytoprotection was blocked by 1 μM mecamylamine. DMXB (1 mg/kg, ip) injected twice but not once per day protected cholinesterase-staining septal neurons from retrograde degeneration following unilateral fimbrial transections. The twice per day DMXB injection-protocol also decreased cell roundness among cholinesterase-staining cells in the lesioned septal hemisphere compared to saline-injected animals. These studies suggest that DMXB may exert cytoprotective activity in NGF-sensitive neuronal populations. © 1994 Wiley-Liss, Inc. 相似文献
4.
采用毛细管流交仪测定了PBT/PC共混物的表观粘度、剪切应力,观察了不同共混物组成和不同温度下共混物的流变行为,并借助扫描电镜对共混物和微观形态结构进行分析。结果表明:PBT/PC熔体共混物的流变行为接近假塑性流体.温度对共混物的流变行为影响很大,共混物的熔体粘度在PBT/PC为90/10和60/40时呈双极值.共混物为两相结构,PC含量为4-50%时呈两互锁结构。 相似文献
5.
利多卡因对N-甲基-D-天冬氨酸抑制大鼠肾上腺嗜铬细胞瘤细胞增殖的影响 总被引:2,自引:0,他引:2
目的 观察利多卡因对 N-甲基-D-天冬氨酸(NMDA)抑制大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)增殖的影响。方法 将体外培养的 PC12细胞分为6组,分别采用正常不含药液的培养基(C组);含400μmol·L(-1)NMDA 的培养基(N组);NMDA 分别混合10μmol·L~(-1)(L_1组)、10~2μmol·L~(-1)(L_2组)、10~3μmol·L~(-1)(L_3组)以及10~4μmol·L~(-1)(L_4组)利多卡因的培养基培养5d,应用流式细胞仪测定细胞 DNA 相对含量,解析细胞周期,计算 S 期细胞荧光强度占受测细胞总荧光强度的百分数为 S期分数(SPF)和 S 期与 G_2期细胞荧光强度之和与 M 期细胞荧光强度的比值[(S G_2)/M]。结果 与C 组比较,N、L_1组 SPF 和(S G_2)/M 均降低(P<0.05),L_4组 SPF 降低(P<0.05),而 L_2及 L_3组 SPF和(S G_2)/M 差异无统计学意义(P>0.05)。与 N 组比较,L_2、L_3及 L_4组 SPF 和(S G_2)/M 升高(P<0.05),L_1组 SPF 升高(P<0.05),而(S G_2)/M 差异无统计学意义(P>0.05)。结论 NMDA 可以通过抑制 PC12细胞 DNA 合成而影响细胞的增殖活性,利多卡因能拮抗 NMDA 对 PC12细胞增殖的抑制作用。 相似文献
6.
目的 克隆人野生型parkin基因并构建真核表达载体pCDNA3.1—parkin,将重组质粒转染PC12细胞获得高表达人野生型parkin基因的PC12细胞克隆。方法 从胎脑组织中提取总RNA,用RT—PCR方法获得人野生型parkin基因的全长cDNA,插入pCR2.1—TA克隆载体中进行序列测定,测序正确后将其亚克隆至表达载体pCD—NA3.1,利用脂质体将重组质粒转染PC12细胞,经G418筛选获得抗性细胞克隆,采用RT—PCR和Western Blot方法鉴定人野生型parkin基因在PC12细胞中的过表达。结果 经限制性内切酶酶切图谱分析和DNA序列测定证实目的基因已插入重组质粒,RT—PCR和Western Blot证明经G418筛选得到的转基因PC12细胞克隆中存在人野生型parkin基因的表达。结论 成功构建了人野生型parkin基因的真核表达载体,获得了稳定表达人野生型parkin基因的PC12细胞克隆,为进一步研究parkin的生物学功能以及parkin在帕金森病发病机制中的作用奠定了良好的基础。 相似文献
7.
Chromaffin cells of the adrenal medulla and their tumor counterparts, the pheochromocytoma (PC12) cells, are well-established
model systems in neurobiology. The development of sympathoadrenal progenitor cells to chromaffin cells can be studied with
regard to developmental signals which trigger the differentiation. With regard to potential treatments of neurological disorders
like Parkinson’s disease chromaffin cell grafting can be used as one therapeutical approach. The beneficial effect of chromaffin
cell grafts is possibly not only related to the release of dopamine but may also be linked to the release of growth factors.
One of the growth factors that is synthesized by chromaffin and PC12 cells is basic fibroblast growth factor (FGF-2). The
experimental data available so far, are in agreement with different functional roles of FGF-2. This article summarizes the
putative physiological functions of FGF-2 in the adrenal medulla. Three differential functional roles of FGF-2 are discussed:
(1) as a differentiation factor for sympathoadrenal progenitor cells; (2) as a target-derived neurotrophic factor for preganglionic
sympathetic neurons which innervate adrenal medullary cells; (3) as an auto-/paracrine factor in the adrenal medulla.
Accepted: 21 August 1996 相似文献
8.
异丙酚对布比卡因诱导的PC12细胞毒性的作用 总被引:1,自引:0,他引:1
目的 探讨异丙酚对布比卡因诱导的PC12细胞毒性的作用及其可能机制。方法PCI2细胞接种于96孔培养板中培养,随机分为4组:对照组、布比卡因组、异丙酚组和布比卡因+异丙酚组,分别加入Hanks液、布比卡因(终浓度为0.03、0.06、0.09、0.12、0.15mmol/L)、异丙酚(终浓度为1、2,4、8mmol/L)以及同时加入布比卡因(终浓度为0.09mmol/L)和异丙酚(终浓度为1、2,4、8mmol/L)。培养24h时,采用二甲基噻唑二甲基四唑溴盐比色微量分析法测定各孔的细胞活力和上清液乳酸脱氢酶(LDH)的活性,应用流式细胞仪检测硫氧还蛋白-1(Trx-1)和硫氧还蛋白还原酶-1(TrxR-1)表达率。结果 与对照组比较,布比卡因组PC12细胞活力降低(P〈0.01),且呈浓度依赖性;异丙酚组PC12细胞活力差异无统计学意义(P〉0.05)。与布比卡因组的0.09mmol/L亚组比较,布比卡因+异丙酚组的2—8mmol/L亚组PC12细胞活力增加(P〈0.05)。与对照组比较,布比卡因组和布比卡因+异丙酚组LDH活性升高,Trx-1和TrxR-1表达率降低(P〈0.01);与布比卡因组比较,异丙酚组和布比卡因+异丙酚组LDH活性降低,Trx-1和TrxR-1表达率升高(P〈0.01)。结论 布比卡因对PC12细胞具有浓度依赖性的细胞毒性作用。异丙酚可通过保护内源性硫氧还蛋白系统减轻布比卡因诱导的PCI2细胞的毒性。 相似文献
9.
本文报告了35例双侧唇裂术后唇部继发畸形。这些畸形主要包括唇红缘不整齐、上唇中份厚度不足以及上唇过紧。Z 字成形、V-Y 推进和 Abbe 瓣法常用于矫正这类继发畸形。 相似文献
10.
M. Vandenbranden J.L. de Coen R. Jeener L. Kanarek J.M. Ruyschaert 《Molecular immunology》1981,18(7):621-631
Interactions between rabbit-γ-immunoglobulins and model membranes (lipid monalayers, planar lipid bilayers, liposomes) have been investigated. No significant interaction was observed with immunoglobulins. However, immunoglobulins dialysed first vs aqueous buffer having pH 2 or 3 and then dialysed against pH 7 buffer presumably adopt a new conformation which allows their bindings to model membranes. This binding is hydrophobic and the immunoglobulin region interacting with the lipid acyl chains is probably located in the heavy chain, as suggested by labelling in this region by a photosensitive probe previously incorporated into the lipid hydrophobic core. Cleavage at the hinge region by papain or pepsin, or heating above 38°C, induces the loss of the hydrophobic conformation responsible for hydrophobic bindings. The binding capacity of immunoglobulins heated above 38°C is restored after momentary dialysis at pH 2. The possible existence of two Ig isomers is discussed in relation to the mechanism of γ-immunoglobulin passage through the endoplasmic membrane and fixation into the plasma membrane. 相似文献