骨疏灵胶囊的质量标准研究

目的：研究骨疏灵胶囊的质量控制标准。方法：采用薄层色谱法对制剂中的补骨脂、女贞子进行定性鉴别；并利用高效液相色谱法对制剂中的淫羊藿苷进行定量分析，以CH3CN-H2O(30：70)为流动相，流速1．0mL／min，色谱柱为C18柱，检测波长为270nm。结果：补骨脂与女贞子的薄层色谱鉴别专属性强，阴性对照无干扰；淫羊藿苷在0.0968～0.4840μgg范围内线性关系良好(r=0．9999)，平均回收率为97．27％(RSD=1．88％，n=5)。结论：该方法可为骨疏灵胶囊的质量评价提供科学依据。     

酸克糖颗粒的质量标准研究

目的：建立酸克糖颗粒的质量控制方法。方法：采用薄层色谱法鉴别其所含山茱萸、白芍，薄层扫描法测定其熊果酸的含量。结果：薄层色谱法鉴别专属性强、重现性好。熊果酸的含量测定线性范围为0．24～1．20μg，回归方程Y=7186．3 X-1356.4，相关系数r=0.9972；加样回收率为100．5％，RSD=2．4％(n=5)。结论：方法操作简便、可行，可用于酸克糖颗粒的质量控制。     

Medulloblastoma in an adult suggestive of external granule cells as its origin: A histological and immunohistochemical study

We describe a rare case of medulloblastoma in an adult woman with histological findings suggesting an origin for this neoplasm in the external granular layer or its remnants. The patient presented with cerebellar dysfunction, and neuroimaging revealed a right cerebellar mass lesion. Pathological examination of the operative specimen revealed a medulloblastoma with occasional areas of neuronal or glial differentiation. Zic protein was also detected immunohistochemically in the tumor cells. The tumor cells were mainly distributed in the subarachnoid space and extended to the cerebellar parenchyma through the perivascular space to form tumor nodules. A suggestive finding, as concerns the origin of this neoplasm, was that the tumor cells were also spread evenly along the subpial zone of the molecular layer, reminiscent of the cellular architecture of the fetal external granular layer.     

重组人白细胞介素-11对小鼠尾部表皮颗粒层形成和血清白细胞介素-2水平的影响

目的观察重组人白细胞介素-11(rhIL-11)对小鼠尾部表皮颗粒层生成和血清白细胞介素-2(IL-2)水平的影响。方法采用小鼠尾部鳞片表皮颗粒层形成模型,不同组别的小鼠给予rhIL-11或甲氨喋呤,观察其对鼠尾鳞片表皮颗粒层和血清IL-2含量的影响。结果rhIL-11可显著促进小鼠尾部鳞片表皮颗粒层形成(P<0.01),并显著抑制血清IL-2的水平(P<0.01)。结论rhIL-11可抑制角质细胞增殖分化不全和减少促发因子IL-2的生成,对银屑病具有较好的防治作用。     

双姜止痛软胶囊质量标准研究

目的:建立双姜止痛软胶囊的质量标准.方法:采用薄层色谱法鉴别双姜止痛软胶囊中干姜及高良姜;高效液相色谱法对制剂中6-姜酚和高良姜素进行定量分析.结果:6-姜酚平均回收率为99.1%,RSD=1.2%(n=9);高良姜素平均回收率为98.8%,RSD=0.78%(n=9).结论:所建立之方法可靠、准确、专属性强,可有效控制双姜软胶囊的质量.     

益肾软肝合剂中赤芍的定性鉴别

[目的]制定益肾软肝合剂的质量标准。[方法]采用薄层色谱法对方中赤芍进行定性鉴别。[结果]供试品色谱在与对照品、对照药材色谱相应的位置上显相同颜色的斑点,且较清晰。[结论]方法灵敏、准确,专属性强,可用于本制剂的定性鉴别和质量控制。     

复方逍遥合剂中黄芩苷的薄层扫描测定

建立了薄层扫描法测定复方逍遥合剂中黄芩苷含量,并采用TLC法对黄柏、白芍、柴胡、贝母等成分进行鉴别.黄芩苷点样量在0.15～0.75μg范围内线性关系良好(r=0.9987),平均回收率99.2%(RSD 1.5%).     

视网膜光凝对糖尿病视网膜病变患者神经纤维层厚度的影响

目的：探讨分析糖尿病视网膜病变(diabetic retinopathy,DR)患者全视网膜激光光凝术(panretinal photocoagulation,PRP)治疗后1a视网膜神经纤维层(retinal nerve fiber layer,RNFL)厚度的变化。

方法：选取2014-05/2017-06在我院行PRP治疗的92例92眼DR患者,采用OCT的DISC CIRCLE程序,测量分析以视盘为中心、直径3.45mm范围DR患者PRP术前和术后1a(采用Follow-Up程序自动追踪模式)视盘周围RNFL厚度,按视盘上方(S)、下方(I)、鼻侧(N)、颞侧(T)4个象限分区所得的数值进行统计分析。

结果：DR患者PRP术后1a较术前视盘周围上方、下方、鼻侧、颞侧和全周平均RNFL厚度明显变薄,且差异有统计学意义(均P<0.05)。

结论：PRP治疗可导致视网膜RNFL变薄,临床上应充分重视和考虑RNFL的损伤,更好地保护视网膜神经细胞的机能对DR治疗具有重要意义。     

ICL植入术后黄斑区节细胞-内丛状层及视网膜厚度的变化

目的：应用OCT观察ICL植入术后黄斑区节细胞-内丛状层及中央区视网膜厚度的改变,明确ICL植入术对于眼后节的影响。

方法：前瞻性研究。最终纳入行ICL植入术患者26例41眼,平均年龄28.19±6.48岁。所有受试者均行眼轴长度(AL)、裸眼视力(UCVA)、综合验光、最佳矫正视力(BCVA)、眼压(IOP)及OCT检查。观察术前及术后的中央区视网膜厚度(CRT)和节细胞-内丛状层厚度(GCT),以及UCVA、BCVA和IOP的变化。

结果： ICL植入术后UCVA和BCVA较术前均有提高(P<0.05),而眼压无变化。术前,术后1wk,1、3mo CRT分别为273.20±25.48、274.07±27.64、277.85±25.49、275.99±24.68μm,而GCT分别为85.31±5.19、88.95±6.87、87.73±4.23、87.45±4.59μm(均P<0.05),其中CRT在术后1mo较术前有增加(P<0.01),GCT在术后1wk,1、3mo均比术前有增加(P<0.05)。GCT在术后1wk的变化与AL呈正相关(r_s=0.529, P=0.001)。

结论：ICL植入术具有良好的有效性及一定的安全性,但术后也会发生一定的黄斑区改变,需给予一定的重视。     

Evaluation of spectral domain optical coherence tomography parameters in discriminating preperimetric glaucoma from high myopia

AIM: To evaluate the diagnostic ability of macular ganglion cell-inner plexiform layer (GCIPL) thickness obtained by spectral-domain optical coherence tomography (SD-OCT) in discriminating non-highly myopic eyes with preperimetric glaucoma (PPG) from highly myopic healthy eyes. METHODS: A total of 254 eyes, including 76 normal controls (NC), 116 eyes with high myopia (HM) and 62 non-highly myopic eyes with PPG were enrolled. The diagnostic ability of OCT parameters was accessed by the areas under the receiver operating characteristic (AUROC) curve in two distinguishing groups: PPG eyes with non-glaucomatous eyes including NC and HM (Group 1), and PPG eyes with HM eyes (Group 2). Differences in diagnostic performance between GCIPL and RNFL parameters were evaluated. RESULTS: The minimum (AUROC curve of 0.782), inferotemporal (0.758) and inferior (0.705) GCIPL thickness were the top three GCIPL parameters in discriminating PPG from non-glaucomatous eyes, all of which had statistically significant lower diagnostic ability than average RNFL thickness (0.847). In discriminating PPG from HM, the best GCIPL parameter was minimum (0.689), statistically significant lower in diagnostic ability than average RNFL thickness (0.789) and three other RNFL thickness parameters of temporal and inferotemporal clock-hour sectors. CONCLUSION: The minimum GCIPL thickness is the best GCIPL parameter to detect non-highly myopic PPG from highly myopic eyes, whose diagnostic ability is inferior to that of average RNFL thickness and RNFL thickness of several temporal and inferotemporal clock-hour sectors. The average RNFL thickness is recommended for discriminating PPG from highly myopic healthy eyes in current clinical practice in a Chinese population.