人脐带源间充质干细胞条件培养液对人肝癌HepG2细胞生长的影响

目的 探讨人脐带源间充质干细胞(HUC-MSC)条件培养液(CM)对人肝癌HepG2细胞生长的影响.方法 选择2011年2月至2014年1月于中国人民解放军第105医院妇产科分娩的35例足月妊娠剖宫产健康胎儿脐带为研究对象,取其用于分离、培养并扩增HUC-MSC.传代扩增至第3代HUC-MSC融合达80％时,更换培养液,继续培养24 h后收集上清液,即为HUC-MSC-CM备用.将对数生长期的人肝癌HepG2细胞按照随机数字表法随机分成相等的3份,分别于含50％,20％及不含HUC-MSC-CM的培养中培养,并分别纳入高含量组、低含量组和空白对照组.采用倒置显微镜观察人肝癌HepG2细胞生长状态,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法和细胞划痕愈合实验分别检测3组人肝癌HepG2细胞增殖和迁移情况,并采用流式细胞术(FCM)法检测各组细胞的细胞周期变化情况.本研究遵循的程序符合中国人民解放军第105医院人体试验委员会所制定的伦理学标准,得到该委员会批准,脐带获取前,均征得孕妇知情同意,并与之签署临床研究知情同意书.结果 ①所分离的原代HUC-MSC培养至第3代后,细胞形态开始比较均一.②MTT法检测结果显示,人肝癌HepG2细胞培养24,48和72 h时,3组相对吸光度(A)值比较,差异均有统计学意义(F=21.330,6.223,9.345;P=0.004,0.032,0.027),且在这3个时间点,高含量组和低含量组相对A值均显著高于空白对照组,差异均有统计学意义(P＜0.05),高含量组相对A值亦显著高于低含量组,差异亦均有统计学意义(P＜0.05).③划痕愈合实验结果显示,3组人肝癌HepG2细胞过河时间比较,差异有统计学意义(F=12.860,P=0.025),且高含量组人肝癌HepG2细胞过河时间短于低含量组和空白对照组,差异均有统计学意义(P＜0.05),低浓度组人肝癌HePG2细胞过河时间亦短于对照组,差异亦有统计学意义(P＜0.05).④FCM法检测人肝癌HepG2细胞周期结果显示,3组G0/G1期和S期人肝癌HepG2细胞比例比较,差异均有统计学意义(F=30.600,30.590;P＜0.05).且与空白对照组相比,高含量组和低含量组人肝癌HepG2细胞G0/G1期细胞比例显著降低,S期细胞比例显著增加,差异均有统计学意义(P＜0.01);且高含量组G0/G1期细胞比例比低含量组下降更显著,而S期细胞比例显著增加,差异均有统计学意义(P＜0.05).结论 HUC-MSC-CM可促进入肝癌HepG2细胞增殖和迁移,并可促进人肝癌HepG2细胞周期G1/S转换.     

Transsphenoidal surgery

Transsphenoidal surgery has evolved much over nearly 100 years. Initially operations were performed often without any input from endocrinology colleagues, and without preoperative imaging, operative magnification and illumination. Advances in the understanding of the biology of pituitary tumours, close co-operation between endocrinologists, surgeons and oncologists, and huge advances in imaging and surgical techniques have led to the evolution of the current transsphenoidal operation to the pituitary fossa to the point where a ‘cure’ is often possible with low complication rates. The indications, contraindications of transsphenoidal surgery will be discussed, together with nature of the surgical approach and how it can be applied to particular pituitary tumours and suprasellar lesions.     

Calcifying fibrous tumor of the gastrointestinal tract: A clinicopathologic review and update

Calcifying fibrous tumor(CFT) is a rare mesenchymal lesion that has been documented throughout the gastrointestinal tract. Gastrointestinal CFTs may occur at virtually any age, with a predilection for adults and for females. They occur most commonly in the stomach and the small and large intestines. CFTs are most often found incidentally, cured by local resection, and have a low risk of recurrence. Histology shows three characteristic features: Spindle cell proliferations within a densely hyalinized stroma, scattered calcifications, and lymphoplasmacytic inflammation. CFTs are immunoreactive for CD34, vimentin and factor XIIIa, helping to distinguish them from other benign mesenchymal neoplasms. The differential diagnosis of CFTs includes sclerosing gastrointestinal stromal tumor, leiomyoma, schwannoma, solitary fibrous tumor, inflammatory myofibroblastic tumor, plexiform fibromyxoma, fibromatosis, sclerosing mesenteritis, and reactive nodular fibrous pseudotumor. The pathogenesis of CFTs remains unclear, but some have hypothesized that they may be linked to IgG4-related disease, inflammatory myofibroblastic lesions, hyaline vascular type Castleman disease, sclerosing angiomatoid nodular transformation of the spleen, or trauma.     

Improved bone regeneration through amniotic membrane loaded with buccal fat pad-derived MSCs as an adjuvant in maxillomandibular reconstruction

BackgroundHuman amniotic membranes (HAMs), as a biological membrane with healing, osteogenic, and cell therapy potential, has been in the spotlight to enhance the outcomes of treating bone defects. Present study aims to clinically assess the potential of HAM loaded with buccal fat pad-derived stem cells (BFSCs) as an osteogenic coverage for onlay bone grafts to maxillomandibular bone defects.Materials and methodsNine patients with jaw bone defects were enrolled in the present study. The patients were allocated to two study groups: Iliac crest bone graft with HAM coverage (n = 5), and Iliac bone grafts covered with HAM loaded with BFSCs (n = 4). Five months following the grafting and prior to implant placement, cone beam computed tomography was performed for radiomorphometric analysis.ResultsThe mean increase in bone width was found to be significantly greater in the HAM + BFSCs group (4.42 ± 1.03 mm versus 3.07 ± 0.73 mm, p < 0.05). Further, the changes in vertical dimension were greater in the HAM + BFSCs group (4.66 ± 1.06 mm versus 4.14 ± 1.03 mm, p > 0.05).ConclusionCombined use of HAM with mesenchymal stem cells may enhance bone regeneration specifically in the horizontal dimension. Moreover, this methodology reduces the amount of harvested autogenous bone and diminish secondary bone resorption.     

人胎盘底蜕膜间充质干细胞抗炎特性对帕金森病模型大鼠的神经保护作用

目的 研究人胎盘底蜕膜间充质干细胞(hPDB-MSCs)抗炎特性对帕金森病(PD)模型大鼠多巴胺能神经元的影响. 方法 体外培养hPDB-MSCs,6-羟基多巴(6-OHDA)制备大鼠PD模型并按照随机数字表法分为模型组与移植组,每组32只.hPDB-MSCs尾静脉移植观察各组大鼠行为学改变.免疫组化检测移植后3d、1周、2周及4周各组大鼠损伤侧黑质酪氨酸羟化酶(TH)、离子钙接头蛋白(Ibal)表达.实时荧光定量PCR检测各时间点各组大鼠损伤侧黑质抗炎因子人白细胞介素-10 (hIL-10)、人转化生长因子-β(hTGF-β)及肿瘤坏死因子-α(TNF-α)表达. 结果 hPDB-MSCs移植后1周、2周、4周,移植组大鼠阿朴吗啡诱导的平均旋转圈数明显低于模型组,差异有统计学意义(P＜0.05).免疫组化结果显示移植组大鼠损伤侧黑质部位TH阳性细胞数较模型组明显增加,1周、2周、4周时差异有统计学意义(P＜0.05).移植组大鼠损伤侧黑质部位Ibal阳性细胞则明显减少,4周时最为显著.mRNA水平,移植组大鼠hIL-10及hTGF-β表达均较模型组增加,而TNF-α表达量则逐渐降低. 结论 hPDB-MSCs尾静脉移植后能够通过抗炎机制抑制PD模型大鼠多巴胺能神经元丧失,改善PD模型大鼠症状.     

Prophylactic academic intervention for children treated with cranial radiation therapy

This single case study investigated the feasibility and effectiveness of a prophylactic intervention for improving academic skills in a child with a brain tumour deemed at high risk for cognitive delay and academic failure because of cranial radiation treatment (CRT). An 8 year old boy participated in a 12 week home and hospital based tutoring programme. Standardized and non-standardized measures of academic achievement were administered at pre- and post-intervention. A follow-up assessment took place 8 months post-intervention (standardized measures only). Pre-test and follow-up neuropsychological data was collected. Significant improvement was observed on the Wechsler Individual Achievement Test–2nd Edition (WIAT-II) pseudoword decoding and spelling sub-tests and on measures of single word and grapheme knowledge. There was no improvement on the WIAT-II math sub-tests. At follow-up, gains were maintained or improved for reading-related sub-tests but declined for math and spelling sub-tests. Overall, neuropsychological data showed decreased performance. The gains in reading skills were made in the context of an overall decline in neuropsychological functioning, suggesting that the intervention helped to preserve reading skills and may be protective against difficulty with skill acquisition, but did not prevent a more global decrease in functioning. This study is the first reported prophylactic intervention delivered concurrently with intensive medical treatment.     

Uterine intravenous lipoleiomyomatosis: uncommon manifestation as a huge retroperitoneal mass with intracaval/intracardiac extensions

Lipoleiomyomas are benign mesenchymal tumours of smooth muscle and adipose tissue and uncommonly occur in the uterus. Here we report a case of uterine lipoleiomyoma that manifested purely as intravenous component and clinically presented as a large retroperitoneal mass with extension into inferior vena cava and right atrium. The connection of the mass to the corneal end of the uterus was noted only at surgery.