Zinc finger of the cerebellum 5 promotes colorectal cancer cell proliferation and cell cycle progression through enhanced CDK1/CDC25c signaling

IntroductionColorectal cancer (CRC), mostly caused by external or environmental factors, is the third most common and lethal cancer worldwide. Although a large number of investigations have been carried out to reveal the evolution of CRC, the underlying mechanisms of CRC remain unclear.Material and methodsExpression of zinc finger of the cerebellum 5 (ZIC5) in CRC tissues and cell models was measured by qRT-PCR and IHC. Cell transfection was carried out for ZIC5 overexpression or knockdown. The MTT assay was applied to examine the capacity of glioma cell proliferation. Wound healing assay and tumor invasion assay were used to test the capacity of glioma cell migration and invasion respectively. Cell cycle analysis and western blot were used to verify the apoptosis rates of CRC cells upon ZIC5 overexpression or downregulation. A further tumor Xenograft study was used to examine the effects of ZIC5 on tumor malignancy in vivo.ResultsCell models using HCT116 and SW620 cells were established to study the ZIC5 function upon ZIC5 overexpression of knockdown. Consistently, we discovered that ZIC5 also significantly increased in Chinese CRC patients. In addition, ZIC5 promoted CRC cell proliferation through increasing the proportion of cells maintained in the S phase. ZIC5 overexpression facilitated the capacity of CRC cell migration and invasion. Inhibition of ZIC5 mitigated such malignant effects.ConclusionsCollectively, investigations of the ZIC5 in CRC provided a new insight into CRC diagnosis, treatment, prognosis and next-step translational therapeutic developments from bench to clinic.     

断指再植术后再植指总体活动度恢复状况及其影响因素分析

目的探讨断指再植术后再植指总体活动度恢复状况及其影响因素分析。方法回顾性选择2018年1月—2020年1月医院收治的断指再植术后患者82例为研究对象,收集汇总患者的性别、年龄、文化程度、术后康复训练情况、缺血时间、术后心理状态、疼痛程度、断离性质、断指伤的原因、断离平面、医保情况、家庭年均收入、职业类型、术后皮肤温度、术后毛细血管反应、术后指腹弹性情况、合并基础疾病、受伤的手指类型等相关因素,进行统计学处理,确定并分析独立影响因素。结果断指再植术后患者的再植指外观恢复得分为15.59±3.45分,运动功能评分为14.27±4.13分,感觉恢复评分为16.38±5.39分,日常活动恢复评分为7.36±2.47分,恢复工作情况评分为6.78±2.59分,再植指功能总分为60.38±16.28分,其再植指功能活动恢复水平较差。单因素分析显示,年龄、术后康复训练、缺血时间、术后心理状态、断离性质、断指伤原因、断离平面、受伤的手指类型、术后指腹弹性、术后皮肤温度均为影响断指再植术后患者再植指功能活动恢复的相关因素(P<0.05)。多元线性回归分析显示,断离平面、术后康复训练、术后心理状况与断指伤原因均为断指再植术后患者再植指功能活动恢复的影响因素(P<0.05)。结论断指再植术患者术后再植指功能恢复与断离平面、断指伤的原因、术后心理状态与术后康复训练情况等密切相关,护理人员配合医师,采取针对性护理干预,促进其功能康复,对患者恢复生活能力、回归工作岗位并重返社会有着极其重要的意义。     

Five-Years Trigger Finger Due to Partial Flexor Tendon Laceration in a Child

Trigger finger (TF) is a condition that affects quality of life and one of the most common causes of hand pain and disability. TF is characterized by catching, snapping or locking of the involved finger flexor tendon, associated with pain. TF in the children occurs rarely than in adults and partial tendon laceration is an uncommon cause of TF in the children. Thus, our aim in this study to define TF due to partial flexor tendon laceration in a child.

Electronic supplementary material

The online version of this article (doi:10.1007/s12593-015-0180-8) contains supplementary material, which is available to authorized users.     

微型金属螺钉治疗骨性锤状指的临床体会

目的 探讨微型金属螺钉治疗骨性锤状指畸形的疗效。方法 12例骨性锤状指畸形患者,采用手指背侧“S”形切口显露骨折断端,应用微型金属螺钉固定末节指骨骨折块。术后以低温塑料夹板行单指固定,4周后去除外固定并开始循序渐进的功能锻炼,早期练习程度以患指轻度肿胀为止,晚期根据X线片显示的骨折愈合情况予以加强,术后随访时采用Dargan功能评定标准评定治疗效果。结果 12例全部获得随访,切口均一期愈合,未出现伤口感染,关节无明显疼痛,通过4～20个月的随访,治疗效果优良率为91.7%。结论 微型金属螺钉治疗骨性锤状指的操作方法简便易行,术后患指可以早期活动,避免了关节僵直及皮肤感染等并发症,功能恢复效果肯定,值得推广。     

锌指蛋白ZBTB20生物学功能的研究进展

锌指蛋白ZBTB20是ZBTB锌指蛋白亚家族的新成员,与已知的BCL6和PLZF同源.通过基因打靶小鼠和疾病人群研究,学者们发现ZBTB20在肝脏、胰岛、神经系统和免疫系统等多个器官与系统中具有重要生理学功能,是个体发育、学习记忆、痛觉感受、糖脂代谢等正常生理过程所必需的调节分子.ZBTB20表达或功能异常可导致个体发育障碍,与智障、肿瘤和代谢性疾病等多种重大疾病的病理生理过程密切相关.作为甲胎蛋白基因的主要转录抑制因子,ZBTB20在出生后肝脏甲胎蛋白基因的表达失活中发挥了关键性作用,且与肝癌预后密切相关.人群中ZBTB20基因的缺失与多种肿瘤有关,其点突变可导致Primrose综合征.本文就ZBTB20的生物学功能研究进展作一综述.     

Knockdown of Gli1 by small-interfering RNA enhances the effects of BCNU on the proliferation and apoptosis of glioma U251 cells

Aims: The present study is to investigate the effect of the combination of small-interfering RNA (siRNA) treatment with bis-chloroethylnitrosourea (BCNU) on the proliferation and apoptosis of glioma cells. Methods: According to different treatments, glioma U251 cells were randomly divided into blank group, Lipofectamine group, siRNA-Gli1 group, BCNU group and combination group. After treatments, the morphology of U251 cells was visualized under the microscope. Afterwards, semi-quantitative real-time polymerase chain reaction and Western blotting were used to determine Gli1, Bcl-2, Bax and cyclin D1 mRNA levels and protein expression, respectively. MTT assay was used detect the proliferation of U251 cells, while flow cytometry was performed to determine cell apoptosis and cell cycle. Results: The combination of siRNA-Gli1 and BCNU caused more severe damages to U251 cell shapes compared with siRNA-Gli1 or BCNU alone. The combination of BCNU and siRNA-Gli1 altered mRNA level and protein expression of Bcl-2 and Bax, but not those of Gli1 and cyclin D1. The combination of siRNA-Gli1 and BCNU promoted U251 cell apoptosis. The combination of siRNA-Gli1 and BCNU enhanced the arrestment of U251 cells in G0/G1 phase. The combination of siRNA-Gli1 and BCNU significantly inhibited U251 cell proliferation. Conclusions: The present study demonstrates that combined treatment with siRNA-Gli1 and BCNU significantly inhibits the proliferation and promotes the apoptosis of glioma U251 cells, possibly by the up-regulation of Bax and the down-regulation of Bcl-2. The combination of siRNA-Gli1 and BCNU enhances the inhibition of cell cycles, but does not down-regulate the expression of cell cycle protein cyclin D1.     

鋅指转录因子ZIC3在内脏异位中的研究现状及进展

内脏异位是由于左右非对称性发育异常所致,常与胸腹腔器官的异常偏侧化有关。心脏经常受累,且心脏受累的严重程度通常决定其预后效果。内脏异位患者有特征性的心血管畸形、内脏器官的异常排列以及中线结构发育畸形。在内脏异位患者中第一个被发现有突变的基因是编码锌指转录因子的ZIC3。很多研究证实,ZIC3突变可导致X连锁内脏异位,而且在孤立性先心病中也发现了ZIC3的突变。至今,在内脏异位患者中发现有13个ZIC3突变,其中包括无义突变、错义突变、沉默突变、移码突变以及易位突变等。然而,ZIC3基因在内脏异位,特别是伴复杂先心病中的致病机理仍不是很清楚。本文就ZIC3结构、作用、突变以及其在内脏异位伴先心病中的研究现状及存在的问题做一综述。