HPLC法测定益心酮片中牡荆素鼠李糖苷的含量

目的：建立益心酮片中牡荆素鼠李糖苷的高效液相色谱含量测定方法；方法：采用岛津VP-OOS（4．6mm×250mm，5μm）色谱柱，流动相为四氢呋喃-异丙醇-1％甲酸溶液（12：4：84），检测波长为340nm；结果：牡荆素鼠李糖苷进样量在1．18—11．80μg之间线性良好，r=0．9998，加样回收率为98．58％，RSD为1．58％；结论：该方法重现性良好，结果准确可靠，可作为评价中成药益心酮片的重要参考方法。     

薄层色谱扫描法测定治带片中苦参碱含量

目的建立治带片的苦参碱含量测定方法。方法采用薄层色谱扫描法，点样于硅胶H板上，以环己烷-乙酸乙酯-丙酮-浓氨试液（4：6：8：0．5）为展开剂，以双波长反射吸收飞点扫描测定（λS =530nm，λR=620nm），外标法定量。结果苦参碱点样量在1-5μg范围内与峰面积线性关系良好，平均加样回收率为99．11％，RSD为3．70％。结论薄层色谱扫描法简单、快速、准确，可以有效控制治带片的质量。     

A study on maize proteins as a potential new tablet excipient

This investigation has examined the use of zein proteins from maize as the major component in oral controlled-release tablets, such formulations often being required to improve patient compliance. Tablets containing ground zein proteins, calcium hydrogen orthophosphate, polyvinyl pyrrolidone, theophylline and magnesium stearate were produced by wet granulation and compression on a single station tablet press and were compared to directly compressed tablets based on zein proteins, calcium hydrogen orthophosphate and theophylline. Non invasive techniques such as Fourier Transform infrared spectroscopy and Fourier Transform Raman spectroscopy were employed to investigate any changes in the secondary structure of zein proteins during tablet production. Random coils, alpha helices and beta sheets predominated and their relative content remained unaffected during grinding, wet granulation and compression, indicating that formulations based on zeins will be robust, i.e. insensitive to minor changes in the production conditions. Drug release from the tablets was studied using a standard pharmacopoeial dissolution test. Dissolution profiles in water, 0.1M HCl (pH=1) and phosphate buffer (pH=6.8) show that only a limited amount of theophylline was released after 4.5h, suggesting that zein proteins could act as a potential vehicle for oral controlled drug release. Analysis of the theophylline release profiles using the Peppas and Sahlin model reveals that diffusion and polymer relaxation occurred in acidic (pH=1) and buffered (pH=6.8) conditions for wet granulated tablets, whereas diffusion was predominant in directly compressed tablets. In conclusion, the present study has shown that zeins can be successfully used as a pharmaceutical excipient, and in particular as a matrix in monolithic controlled release tablets.     

劳损康复片抗急性软组织损伤药效学研究

目的:观察劳损康复片抗急性软组织损伤的作用。方法:受试药采用灌胃给药途径,连续给药7d,劳损康复片给药剂量小鼠分别为3.0,10.0,30.0(生药)g·kg-1;大鼠分别为2.2,7.5,22.0(生药)g·kg-1。采用热板法和醋酸扭体法观察镇痛作用;二甲苯致小鼠耳肿胀和角叉菜胶致大鼠足肿模型观察抗炎作用;自由落体组织损伤模型观察抗急性软组织损伤作用。结果:劳损康复片显著提高热板法小鼠的痛阈值、减少醋酸所致的扭体次数(P<0.01);明显降低二甲苯所致小鼠耳肿胀度;7.5,22.0(生药)g.kg-1剂量组明显抑制角叉菜胶引起的大鼠足肿胀度;明显降低损伤大鼠的表观损伤积分,改善损伤组织的病理学表现。结论:劳损康复片具有抗急性软组织损伤的作用。     

奈普生栓联合米索前列醇片在早孕人工流产术中的应用

目的：探讨奈普生栓联合米索前列醇片在早孕人工流产术中扩张宫颈及镇痛作用。方法：随机将病例分为两组，A组：肛塞奈普生栓1枚及口服米索前列醇片1片30min后行人工流产术50例。B组：宫颈采用直径为8mm的棉签浸湿2％丁卡因放置于进宫颈内口处5min后行人工流产术50例。结果：A组用药后宫颈扩张效果良好，与B组比较有显著性差异（P〈0.01）。结论：奈普生栓联合米索前列醇片在早孕人工流产术中有较好的扩张宫颈及镇痛作用，且人工流产综合征发生率较低。     

高效液相色谱法测定黏膜溃疡含片中儿茶素和表儿茶素的含量

目的建立黏膜溃疡含片中儿茶素和表儿茶素的含量测定方法。方法采用高效液相色谱法,以0.04 mol.L-1枸橼酸-N,N-二甲基甲酰胺-四氢呋喃(45∶8∶1)为流动相,检测波长265 nm。结果儿茶素和表儿茶素分别在0.103~2.568μg(r=0.9999)和0.011~0.275μg(r=0.9999)范围内线性关系良好,平均回收率分别为102.17%和99.84%,RSD分别为1.69%和1.44%。结论该方法简便、准确、重现性好,可用于该制剂的质量控制。     

高效液相色谱法测定黄金咽喉片中甘草酸的含量

目的建立黄金咽喉片中甘草酸的含量测定方法。方法采用HPLC法测定甘草酸的含量。色谱柱为Dikma Kro-masil C18柱(5μm,250 mm×4.6 mm);流动相甲醇-0.2 mol/L醋酸铵-冰醋酸(66∶34∶1);流速0.8 ml.min-1;检测波长252 nm;柱温40℃;进样量10μl。结果甘草酸在0.4-4μg范围内具有良好的线性关系(r=0.999 9),平均回收率为99.65%,RSD为0.514%(n=6)。实验测得黄金咽喉片中甘草酸的含量为0.978-1.086 mg/片。结论该方法简便、快速、有效、灵敏、准确、具有良好的重复性和回收率,可作为该制剂的定量分析方法。     

高效液相色谱法测定止嗽片中橙皮苷的含量

目的:应用高效液相色谱法对止嗽片中橙皮苷进行含量测定。方法:色谱条件为Aglient SB-18(4.6×150mm),流动相为乙腈-0.3%磷酸溶液(20:80),UV检测波长为283nm。结果:此方法线性关系良好,平均加样回收率为98.7%(n=6)。结论:高效液相色谱法测定止嗽片中橙皮苷含量的方法准确,回收率好。     

山楂西洋参疏肝口崩片中总皂苷的含量测定

目的建立测定山楂西洋参疏肝口崩片中西洋参总皂苷含量的紫外分光光度法。方法以人参皂苷Rg1为对照品，香草醛-冰醋酸溶液-高氯酸溶液为显色剂，测定波长为540nm。结果人参皂苷Rg1的含量在19．32～120．75μg范围内与吸收度线性关系良好（r=0．9997），平均回收率为99．36％，RSD=1．76％（n=6）。结论所用方法操作简便，灵敏度高，重现性好，能准确测定山楂西洋参疏肝口崩片中总皂苷的含量。     

五氟利多片微生物限度检查方法的建立

目的建立五氟利多片的微生物检查方法。方法采用5种阳性对照菌回收率试验测定其抑菌作用的消除效果。结果根据回收率试验结果，必须采用离心集菌及薄膜过滤法才能彻底消除其抑菌作用。结论应按离心集菌薄膜过滤法对其进行微生物限度检查。