Photocrosslinkable laminin-functionalized polyethylene glycol hydrogel for intervertebral disc regeneration

Intervertebral disc (IVD) disorders and age-related degeneration are believed to contribute to lower back pain. There is significant interest in cell-based strategies for regenerating the nucleus pulposus (NP) region of the disc; however, few scaffolds have been evaluated for their ability to promote or maintain an immature NP cell phenotype. Previous studies have shown that NP cell–laminin interactions promote cell adhesion and biosynthesis, which suggests a laminin-functionalized biomaterial may be useful for promoting or maintaining the NP cell phenotype. Here, a photocrosslinkable poly(ethylene glycol)–laminin 111 (PEG-LM111) hydrogel was developed. The mechanical properties of PEG-LM111 hydrogel could be tuned within the range of dynamic shear moduli values previously reported for human NP. When primary immature porcine NP cells were seeded onto PEG-LM111 hydrogels of varying stiffnesses, LM111-presenting hydrogels were found to promote cell clustering and increased levels of sGAG production as compared to stiffer LM111-presenting and PEG-only gels. When cells were encapsulated in 3-D gels, hydrogel formulation was found to influence NP cell metabolism and expression of proposed NP phenotypic markers, with higher expression of N-cadherin and cytokeratin 8 observed for cells cultured in softer (<1 kPa) PEG-LM111 hydrogels. Overall, these findings suggest that soft, LM111-functionalized hydrogels may promote or maintain the expression of specific markers characteristic of an immature NP cell phenotype.     

冠心病心功能不全患者PCⅢ、LN、HA的变化及对比研究

目的::探讨冠心病病人心功能不全改善前后血清Ⅲ型前胶原(PCⅢ)、层粘连蛋白(LN)、透明质酸(HA)的含量变化及其临床意义。方法:将78例冠心病病人分为观察组和对照组,观察组行冠状动脉造影及左心室造影和超声心动图检查,并测定左室功能,术后每天用异舒吉20mg,共用14d,治疗结束后再次行超声心动图检查测定左心室功能。两组病人于治疗前后分别测定血清PCⅢ、LN、HA的浓度变化。结果:观察组病人的血清PCⅢ、LN、HA浓度较对照组明显增加(t=14.17~16.69,P<0.01),观察组病人治疗后血清PCⅢ、LN、HA浓度较治疗前明显下降(t=2.352~4.271,P<0.05,0.01)。结论:血清PCⅢ、LN、HA在冠心病心功能不全的发生、发展中起重要的作用,具有一定的临床参考价值。     

HIF-1a,VEGF和LN-R在胃肠间质瘤中的表达及其意义

目的 探讨缺氧诱导因子(HIF-1α)、血管内皮生长因子(vEGF)和层粘连蛋白因子受体(LNR)在胃肠间质瘤(GISTs)中的表达及其在预后判断中的价值.方法 应用免疫组织化学S-P法和Envision二步法方法对95例GISTs检测,观察HIF-1a,VEGF和LN-R.在GISTs中的表达情况.结果 按Flecther标准将95例GISTs分为极低风险(2例),低风险(12例),中度风险(53例)和高度风险(28例),HIF-1a,VEGF的表达率在极低和低风险,中度风险和高度风险GISTT中的表达逐渐升高,3组比较有较显著差异(P＜0.05);而LN-R仅7例表达,在侵透浆膜的中度恶性和高度恶性中占6(83.7%)例表达.肿瘤按直径＜5cm,5～10 cm,＞10cm和核分裂＜5/50HPF,5～10/50HPF,＞10/50HPF分为三组,HIF-1a,VEGF的表达也是渐升高的趋势,且3组比较有较显著差异(P＜0.05).结论 HIF-1a,VEGF和LN-R在GISTs中的表达可能提示肿瘤的恶性程度及其预后,并可作为判断预后的有用标志物.同时可能给GISTs的抗血管分子治疗提供科学依据.     

肝纤维化指标的检测及临床意义

目的 研究血清纤维化指标透明质酸（HA）、IV型胶原（Ⅳ-c）、Ⅲ型前胶原肽（PCⅢ）、层粘连蛋白（LN）水平与肝纤维化指标的关系.方法 对确诊的慢性乙型肝炎及肝硬化患者124例和健康人18例,测定血清纤维化指标水平,33例患者进行肝穿行肝组织炎症及肝纤维化分期.血清纤维化指标行统计学分析.结果 血清中HA与CIV水平在慢性肝炎各相平行,而LN与PCⅢ在慢性肝炎病理分期相同时结果一致,在G4期时PCⅢ结果与正常对照相近.四项指标与对照组比较有明显升高.结论 血清纤维化指标与肝纤维化有一定的相关性,这些指标与临床结合对肝纤维化有一定的价值.LN与PCⅢ在慢性肝炎患者分期G_4前与纤维化程度相一致,联合多项指标检测可在一定程度上提高肝纤维化诊断.     

Animal study on expression of laminin and fibronectin in cornea during wound healing following alkali burn

Objective:To observe the expression of laminin and fibronectin in alkali-burned corneas in rats.Methods:A total of 18 normal Wistar rats were randomly divided into 6 groups(n=3 in each group).For each rat, one eye was injured by alkali burn,the other one was taken as the normal control.Then all the corneas were surgically removed and the expression of laminin and fibronectin was observed with immunohistochemistry respectively at 7 hours,1 day,3 days, 7 days,14 days and 28 days after alkali burn.Results:Compared with that of the normal controls, the expression of laminin and fibronectin of the burned eyes was dramatically higher at 7 hours, reached peak at 14 days and decreased to the normal level at 28 days after alkali burn.Conclusions:In the process of wound healing after alkali burn, the expression of laminin and fibronectin increases dramatically, which suggests that laminin and fibronectin may participate in the process of corneal wound healing.     

细胞外间质成分与肝病关系的研究

利用固相酶联免疫检测技术（ＥＬＩＳＡ），建立了层粘连蛋白（Ｌａｍｉｎｉｎ，ＬＮ）和透明质酸（Ｈｙａｌｕｒｏｎａｔｅ，ＨＡ）的检测方法，对１３８例肝病患者的血清含量进行了分析。结果发现，ＬＮ和ＨＡ随着肝病程度的加重而逐步升高，急性肝炎时ＨＡ含量轻度高于正常人（Ｐ＜０．０５），ＬＮ变化不显著。慢性肝病时ＬＮ与ＨＡ血清含量均明显升高（Ｐ＜０．０５～０．００１），于肝硬化时达到最高。以慢性活动性肝炎的上限值为界，ＬＮ和ＨＡ联合诊断肝硬化的敏感性、特异性和诊断符合率均为９０％，提示ＬＮ和ＨＡ是判断慢性肝病损伤程度和诊断肝纤维化的良好指标。     

糖尿病大鼠糖基化终产物与主动脉细胞外基质成分的关系

为探讨糖基化终产物在糖尿病大鼠动脉粥样硬化发生中对主动脉细胞外基质成分生成增加所起的作用,本实验将糖尿病大鼠、糖尿病＋ 氨基胍治疗大鼠和正常大鼠分别喂养１、２、３ 和４ 个月后,测定其血红蛋白－ 糖基化终产物及主动脉壁Ⅲ型前胶原、Ⅳ型胶原、Ⅳ型胶原－ 糖基化终产物及层粘蛋白含量。结果发现,糖尿病组大鼠各时相点血红蛋白－ 糖基化终产物（１ ～４ 个月分别为６．８８±１ ．２３、１０ ．２６±０．６３、１５．３±１．４９ 和１８．５７ ±２．９０ ｋｕｇ, 与血糖水平相关）、Ⅲ型前胶原（１ ～４个月分别为１５．２０±３．０３ 、２１．４４ ±１．７９、２７．１９±３ ．２８ 和３３．９９±４ ．９６ μｇＬ, 与血红蛋白－ 糖基化终产物和Ⅳ型胶原－ 糖基化终产物相关） 、Ⅳ型胶原（１～４ 个月分别为２３．６７±１．４９、３０．３７ ±２ ．８６、３６．６５ ±１．９８ 和４５．４６±５．７７ μｇＬ, 与血红蛋白－ 糖基化终产物和Ⅳ型胶原－ 糖基化终产物相关）、Ⅳ型胶原－ 糖基化终产物（１～４ 个月分别为０．７９ ±０．１５、１．２５ ±０．２２ 、１．５４ ±０．０６ 和１．８０±０ ．１４ Ｍｕｇ, 与血红蛋白－ 糖基化终产物和Ⅳ型胶原相关）      

精液中HA、LN、PCⅢ、CⅣ含量与精子质量关系的研究

目的:探讨精液中透明质酸(HA),层粘连蛋白(LN),Ⅲ型前胶原(PCⅢ),IV型胶原(CⅣ)与男性精子质量的关系。方法:采用放射免疫分析法分别检测60例男性生育者和不育者精液中的HA、LN、PCⅢ、CⅣ,并进行精子质量分析。结果:精液液化时间正常与异常组精液中HA、PCⅢ、CⅣ及LN含量差异无统计学意义;精子密度正常组精液中HA、PCⅢ及LN含量明显高于异常组,两组比较有统计学意义(P<0.01),而两组中CⅣ含量比较差异无统计学意义(P>0.05);精子活动率异常组精液中HA和LN含量明显高于正常组,两组比较差异有统计学意义(P<0.01),而两组中的PCⅢ和CⅣ含量比较差异无统计学意义(P>0.05)。结论:精液中HA、LN的升高可能是生精功能活跃的表现,且不排除HA、LN是刺激男性生精因素的可能,测定精液中HA、LN的含量可以间接了解生育能力。     

The presence of laminin in the fetal human inner ear

Summary The expression of laminin was analyzed in the human fetal inner ear using immunohistochemical methods. In the 11-week-old human fetus, the presence of laminin was found in the basement membrane of the immature cochlea, endolymphatic sac and vestibular end organs. The reaction of the basement membrane of the endolymphatic sac was strong in the 15-week-old human fetus. A laminin reaction was seen in the cochlea, Reissner's membrane, epithelial cells of the limbus spiralis, the basilar membrane and the stria vascularis. In particular, the capillaries and basement membrane of the stria vascularis were strongly positive. These results suggest that laminin may be an essential component in the development of the inner ear and may possibly be related to filtration of the endolymph.     

恶性肿瘤患者血清层粘蛋白含量的初步研究

利用酶联免疫吸咐法（ＥＬＩＳＡ）检测了１３５例恶性肿瘤患者清中层粘蛋白的含量。比较了良恶性肿瘤及健康成人血清ＬＮ的差别，对ＬＮ在癌变中的机制进行了初步探讨。结果表明，所有恶性肿瘤患者清ＬＮ值均高于健康对照（Ｐ＜０．００１），并且高于良性肿瘤患者（Ｐ＜０．００１），良性肿瘤和健康组之间没有差别（Ｐ＞０．０５）。以正常人ＬＮ值上限为界，恶性肿瘤阳性分布率为７４．８％，良性肿瘤１３％，正常人假阳性为８．