Osteoblasts induce Ca2+ oscillation-independent NFATc1 activation during osteoclastogenesis

Intercellular cross-talk between osteoblasts and osteoclasts is important for controlling bone remolding and maintenance. However, the precise molecular mechanism by which osteoblasts regulate osteoclastogenesis is still largely unknown. Here, we show that osteoblasts can induce Ca(2+) oscillation-independent osteoclastogenesis. We found that bone marrow-derived monocyte/macrophage precursor cells (BMMs) lacking inositol 1,4,5-trisphosphate receptor type2 (IP(3)R2) did not exhibit Ca(2+) oscillation or differentiation into multinuclear osteoclasts in response to recombinant receptor activator of NF-kappaB ligand/macrophage colony-stimulating factor stimulation. IP(3)R2 knockout BMMs, however, underwent osteoclastogenesis when they were cocultured with osteoblasts or in vivo in the absence of Ca(2+) oscillation. Furthermore, we found that Ca(2+) oscillation-independent osteoclastogenesis was insensitive to FK506, a calcineurin inhibitor. Taken together, we conclude that both Ca(2+) oscillation/calcineurin-dependent and -independent signaling pathways contribute to NFATc1 activation, leading to efficient osteoclastogenesis in vivo.     

曲妥珠单抗联合IP方案或SOX方案化疗在人类表皮生长因子受体-2阳性晚期胃癌中的有效性和安全性比较

目的 探讨人类表皮生长因子受体-2(HER-2)阳性晚期胃癌中曲妥珠单抗联合IP方案或SOX方案化疗的有效性和安全性。方法 连续性纳入自2015年6月至2017年6月内蒙古医科大学附属医院收治的58例HER-2阳性晚期胃癌,利用随机数字表法分为SOX组和IP组,每组29例。SOX组给予曲妥珠单抗联合替吉奥+奥沙利铂治疗;IP组给予曲妥珠单抗联合伊立替康+顺铂治疗,比较两个疗程后两组病人血清肿瘤标志物和新生血管标志物的变化,以及化疗效果和不良反应差异。结果 两个治疗疗程后,两组病人血清肿瘤标志物癌胚抗原(CEA),糖类蛋白19-9(CA19-9),糖类抗原125(CA125)以及组织多肽特异性抗原(TPS)水平均差异无统计学意义(t=0.628,P=0.532;t=0.879,P=0.383;t=0.828,P=0.411;t=0.719,P=0.476);新生血管标志物内皮生长因子(VEGF)、血管生成素-2(Ang-2)、内皮抑素(ES)、色素上皮衍生因子(PEDF)水平亦差异无统计学意义(t=0.701,P=0.486;t=0.955,P=0.343;t=1.803,P=0.077;t=0.991,P=0.326;);SOX组客观有效率(ORR)及病控制率(DCR)分别为44.83%和82.76%;IP组ORR及DCR分别为34.48%和69.97%,均差异无统计学意义(χ²=0.648,P=0.421;χ²=1.506,P=0.219)。但IP组骨髓抑制(58.62%)和恶心呕吐(3.45%)发生率明显高于SOX组(31.03%,6.90%),差异有统计学意义(χ²=4.461,P=0.035;χ²=5.836,P=0.016)。结论 曲妥珠单抗联合SOX化疗方案或IP方案在HER-2阳性晚期胃癌病人中疗效相当,但SOX方案不良反应发生率较少,值得临床推广。     

电话随访系统在出院随访中的应用

以镇江市第四人民医院为例,阐述随访系统的积极作用,包括完善随访管理流程、增强随访监控及实现数据汇总,分析存在的问题并提出相应改进措施,包括建立专业随访系统开发团队、实现各系统间数据对接等方面。     

胃肠道平滑肌起搏电流产生机制的研究进展

越来越多的研究结果表明,Cajal间质细胞是胃肠道平滑肌慢波电位和自发性节律性收缩活动的起搏细胞.关于Cajal间质细胞起搏电流产生机制的研究近来进展很快,但仍存在一些分歧.本文就目前关于起搏电流的产生及传播机制的研究进展作一综述.     

Prognostic value of serial serum KL-6 measurements in patients with idiopathic pulmonary fibrosis

Background

The prognostic significance of serial measurements of serum KL-6 levels in patients with idiopathic pulmonary fibrosis (IPF) is unclear; hence, it was assessed in this study.

刚地弓形虫MIC8 CTD作用蛋白的筛选及鉴定

目的从刚地弓形虫速殖子裂解液中筛选并鉴定微线体蛋白8羧基端胞质尾(MIC8CTD)的作用蛋白。方法分别以GST-MIC8CTD蛋白和GST蛋白(对照)作为探针蛋白,采用GST pull-down技术从弓形虫裂解液中筛选目标蛋白并进行SDS-PAGE分析;将目标蛋白转印至PVDF膜,测序,通过BLAST2在线对比搜索相似蛋白序列,初步确定目标蛋白;以目标蛋白抗体为一抗进行Western blot,分析GST pull-down产物与目标蛋白抗体的相互作用;分别用GSTMIC8CTD多克隆抗体和兔免疫前血清结合的sepharose与弓形虫裂解液进行免疫共沉淀试验,沉淀物进行SDS-PAGE和Western blot分析。结果 PAGE显示GST-MIC8CTD蛋白的pull-down产物中有一蛋白条带(分子质量单位约35~45ku),GST蛋白的pull-down产物中无此蛋白;BLAST2比对目标蛋白的氨基酸序列与弓形虫醛缩酶序列一致;ECM检测GST-MIC8CTD多克隆抗体的免疫共沉淀产物中有蛋白组分可被相应的抗体识别,免疫前血清的免疫共沉淀产物中无可被识别的蛋白。结论经GST pull-down技术和免疫共沉淀试验筛选、鉴定,弓形虫MIC8CTD的作用蛋白为醛缩酶。     

Reduced area of the normal lung on high-resolution computed tomography predicts poor survival in patients with lung cancer and combined pulmonary fibrosis and emphysema

Background

This study aimed to determine the radiologic predictors and clarify the clinical features related to survival in patients with combined pulmonary fibrosis and emphysema (CPFE) and lung cancer.

艾滋病人腹泻粪便中隐孢子虫的检测方法探讨

[目的]选择一种便捷实用的方法检测隐孢子虫,为艾滋病患者机会性感染引起隐孢子虫病的诊断治疗提供实验室依据.[方法]186份标本,每份一式两用.分别用硫酸锌离心浮聚＋碘染法（硫-碘法）和甲醛-乙酯乙酸沉淀集菌＋改良Ziehl-Neelsen染色法（改良法）检测隐孢子虫.[结果]硫-碘法阳性检出率为19.4%,改良法阳性检出率为44.1%.两方法相比较差异有统计学意义（χ2=38.142,P〈0.01）.[结论]甲醛-乙酸乙酯沉淀集菌＋改良Ziehl-Neelsen染色法染色效果佳,对艾滋病人腹泻粪便中隐孢子虫的检测更敏感,适合于基层医院推广使用.