Tissue engineering of tendons and ligaments by human bone marrow stromal cells in a liquid fibrin matrix in immunodeficient rats: Results of a histologic study

Introduction The original complex structure and mechanical properties are not fully restored after ligament and tendon injuries. Due to their high proliferation rate and differentiation potential, Bone Marrow Stromal Cells (BMSC) are considered to be an ideal cell source for tissue engineering to optimize the healing process. Ideal matrices for tissue engineering of ligaments and tendons should allow for homogenous cell seeding and offer sufficient stability. Material and methods A mixture of human BMSC and liquid fibrin glue was injected into a standardized full-thickness window defect of the patellar tendon of immunodeficient rats (BMSC group). The histology of the tissue was analysed 10 and 20 days postoperatively and compared to four control groups. These groups consisted of a cohort with a mixture of human fibroblasts and fibrin glue, fibrin glue without cells, a defect group without treatment, and a group with uninjured patellar tendon tissue. Results Tendon defects in the BMSC group revealed dense collagen fibres and spindle-shaped cells, which were mainly orientated along the loading axis. Histologic sections of the control groups, especially of untreated defects and of defects filled with fibrin glue only, showed irregular patterns of cell distribution, irregular formed cell nucleoli and less tissue maturation. Compared to healthy tendon tissue, higher numbers of cells and less intense matrix staining was observed in the BMSC group. No ectopic bone or cartilage formation was observed in any specimen. Conclusions Injection of human BMSC in a fibrin glue matrix appears to lead to more mature tissue formation with more regular patterns of cell distribution. Advantages of this “in-vivo” tissue engineering approach are a homogenous cell-matrix mixture in a well-known and approved biological matrix, and simple, minimally-invasive application by injection.     

美宝创面速愈贴治疗糖尿病足溃疡的临床疗效观察

目的评价美宝创面速愈贴治疗糖尿病足溃疡的有效性。方法共纳入11例患者,随机分为两组,在均继续给予胰岛素和(或)口服降糖药控制血糖及降血脂、血压,同时给予抗血小板,改善微循环,营养神经抗感染等治疗前提下,分别使用美宝创面速愈贴(治疗组,n=6)和常规换药法(对照组,n=5)局部处理糖尿病足溃疡,观察12周。结果治疗组溃疡愈合、显效率明显优于对照组(P<0.05)。结论美宝创面速愈贴用于治疗糖尿病足溃疡有较好疗效。     

碱性成纤维细胞生长因子对宫颈上皮内瘤变术后创面愈合的临床疗效观察、

目的观察外用重组碱性成纤维细胞生长因子耐宫颈上皮内瘤变术后创面愈合的临床疗效。方法实验组30例患者LEEP术后予碘伏消毒后用重组碱性成纤维细胞生长因子进行创面换药治疗,对照组30例患者行碘伏消毒创面,比较两组愈合时间、创面愈合率及术名并发症的发生率。结果实验组较对照组愈合时间明显缩短,差异有统计学意义（P〈0．01）;实验组与对照组术后第1周、第2周和第3周的创面愈合率差异均具有统计学意义（P〈0．01）。实验组和对照组在不规则阴道流血、宫颈疤痕软硬度、阴道排液量和时间方面差异有统计学意义（P〈0．05）;但在阴道流血量和术后感染率方面差异无统计学意义（P〉0．05）：结论外用重组碱性成纤维细胞生长因子对宫颈上皮内瘤变术后创面愈合具有很好的促进作用,可以缩短愈合时间,增加创面愈合率,并减少不规则阴道出血率、阴道排液量及时间。     

中药药线对口引流法在复杂性肛瘘术后创面愈合观察

目的观察中药药线对口引流法在治疗复杂性肛瘘术后促进创面愈合的临床疗效。方法将60例复杂性肛瘘患者按随机数字表法分为治疗组30例,采用中药药线对口引流法,对照组30例采用切开旷置术,观察术后不同时间点创面愈合率、肉芽生长及治愈率情况。结果与对照组比较,治疗组术后不同时间点肉芽生长明显改善（P〈0．05）,愈合率在第10、14、20天差异有统计学意义（P〈0．05）;治疗组总有效率高于对照组,差异有统计学意义（χ^2=4．043,P〈0．05）。结论中药药线对口引流法治疗复杂性肛瘘,能加快肉芽生长,促进创面愈合,提高临床疗效。     

定量CT评价低强度超声仪促进桡骨远端骨折愈合效果及价值分析

目的研究定量CT评价低强度超声仪（LIUS）用于治疗桡骨远端骨折的临床疗效以及价值。方法选取2009年7月～2010年7月治疗的闭合性桡骨远端骨折患者46例,将患者按照治疗方法分为观察组和对照组,每组各23例,观察组采取超声治疗,1次/d,20 min/次,超声平均强度水平为30 mW/cm2,对照组采取常规切开复位内固定治疗,在治疗的第2、6、10周利用CT对两组患者骨折端附近3 cm处的骨段、体模进行扫描,对两组临床愈合时间、骨性愈合时间、骨折端力学强度指数（BSI）、皮质骨骨密度（cBMD）进行比较。结果对照组临床愈合时间、骨性愈合时间均长于观察组,两组间比较,差异有统计学意义（均P〈0.05）。观察组和对照组第2、6、10周时BSI、cBMD差异均有统计学意义（均P〈0.05）。观察组患者第2、6周时的BSI、cBMD均优于对照组,差异有统计学意义（均P〈0.05）;第10周时观察组患者BSI、cBMD与对照组比较,差异无统计学意义（均P〉0.05）。结论 LIUS能够有效促进患者骨折端快速愈合,定量CT可以为患者的治疗提供详细的、精确的治疗依据。     

糖尿病皮肤缺损愈合进程中单核细胞趋化蛋白-1表达的研究

目的研究单核细胞趋化蛋白-1(Monocyte chemoattractant protein-1,MCP-1)在兔糖尿病皮肤缺损模型愈合进程中的表达水平,探讨其在糖尿病皮肤愈合中的作用.方法制作兔糖尿病模型,通过免疫组化和ELISA的方法研究MCP-1在兔糖尿病皮肤缺损模型愈合进程中的局部表达及血清中水平.结果免疫组化染色提示MCP-1在糖尿病组中各时间点位表达均高于空白对照组.外周血中造模后3 d和1周时MCP-1含量糖尿病组与空白组比较有统计学意义(P<0.05),2周和4周时无统计学意义(P>0.05).结论 MCP-1可能在糖尿病皮肤缺损修复愈合紊乱的炎性反应中发挥作用,最终干扰愈合.     

The effect of healing touch on the pain and mobility of persons with osteoarthritis: A feasibility study

The aims of this pilot study were to investigate the effects of Healing Touch (HT) on the pain level, joint function, mobility, and depression in persons with osteoarthritis (OA) of the knee joint(s). A randomized controlled trial using a repeated measures design was used. Cognitively intact persons (institutionalized and community) with a diagnosis of OA of the knee joint(s) received either HT sessions three times per week for 6 weeks (n = 12) or weekly friendly visits (FV) (n = 7). The HT sessions were delivered by a team of two nurses certified as HT practitioners and the FV was conducted by a nurse. All subjects continued to receive their standard care including the methods they had been using to relieve their joint pain. The two groups were similar regarding demographic variables, number of knees affected, co-morbidities, pain medications used and outcome variables at baseline. Two pain outcome measures (intensity and life interference) produced significant interaction effects. Two joint outcome measures (extension and extensor lag) also produced significant interaction effects. Furthermore, the HT group demonstrated significant improvements in 9 of the 12 outcome variables (75%) while no significant improvements occurred in the FV group. The HT group exhibited sustained effects (3 weeks post treatments) in three outcome variables. The reduction in joint pain and improvement in joint function suggest that biofield therapies could be effective non-pharmacological adjuncts to treatment of OA.     

脂联素在脂肪组织切口愈合中的作用及机制研究

目的 观察脂联素在脂肪组织切口愈合（ATWH）中的作用,探讨脂联素在ATWH中的作用机制.方法 制作猪ATWH模型;随机分为脂联素干预组和对照组（各组n=5）,观察ATWH等级,分析脂联素对ATWH的影响.应用免疫荧光标记技术和电镜观察脂肪细胞凋亡变化情况,分析脂联素水平对脂肪细胞凋亡的影响.结果 干预组切口愈合等级显著优于对照组（P〈0.05）;干预组脂肪细胞凋亡率明显少于对照组（P〈0.05）.结论 脂联素通过抑制脂肪细胞的凋亡促进ATWH.     

烫伤大鼠创面组织中P物质含量的动态变化

目的　观测神经肽P物质 (SP)在不同深度烫伤大鼠创面中含量的动态变化。方法　采用大鼠烫伤模型 ,根据烫伤时间随机分为 4 ,8,12s烫伤组 (每组 2 4只 ) ,每组于伤后 2 4h ,3d ,7d ,14d各处死 6只大鼠 ,放射免疫法测定创面组织SP含量。结果　伤后 2 4h ,浅Ⅱ度、深Ⅱ度、Ⅲ度烫伤创面SP含量分别为 (14 5. 35 7± 78 .5 38) ,(94 . 6 72± 4 8. 6 74 ) ,(5 3 .6 73± 2 7. 6 5 8)mg ,两组之间差异有显著性。浅Ⅱ度创面伤后 3d、7dSP含量显著增高 ;深Ⅱ度创面SP含量伤后 7d及 14d显著增高 ;Ⅲ度创面SP含量伤后无显著性变化。结论　SP含量与烫伤创面愈合能力正相关。