BASIC FIBROBLAST GROWTH FACTOR GENE TRANSFECTION TO ENHANCE THE REPAIR OF AVASCULAR NECROSIS OF THE FEMORAL HEAD

A VASCULAR necrosis of the femoral head usually leads to destruction of the hip joint, increasing musculoskeletal morbidity in young and middle aged patients. Total joint replacement is commonly used to treat disabling secondary osteoarthrosis. Although most recent improvements in total hip replacement may decrease the failure, patients are usually young and the current hip prothesis function would not fulfill purpose for the remaining life ex-pectancy for these patients. So it is necessary…     

宁痫冲剂对戊四唑致痫大鼠脑源性神经营养因子蛋白表达的影响

[目的]探讨以升清降浊法组成的中药复方宁痫冲剂的抗痫作用机理。[方法]SD大鼠40只,随机分为5组:即正常组,模型组,宁痫冲剂高、低剂量组(10g/kg和5g/kg)及苯巴比妥组(60 mg/kg);除正常组外,其他4组均以戊四唑(PTZ)亚惊厥剂量35mg/kg诱导慢性癫痫模型,造模同时各组按设定剂量分别给药,1次/d,连续4周;动物处死后以免疫组织化学方法,观察宁痫冲剂对大脑额叶皮质、海马CA3区脑源性神经营养因子(BDNF)蛋白表达的影响。[结果]模型组大鼠脑内海马、皮质区免疫标记阳性神经元数目均增多(P<0.05或P<0.01),标记强度增强;宁痫冲剂低剂量组能减少免疫阳性神经元数目(P<0.05或P<0.01),减弱免疫反应性。[结论]PTZ慢性诱导癫痫反复发作可导致脑内BDNF表达增强;宁痫冲剂的抗痫作用机制可能与降低BDNF蛋白表达有关。