谷子种子经高空气球搭载后的遗传变异研究

目的 选育出高产、蛋白质和用脂肪含量高的谷子品（系）种。方法 利用高空气球搭载谷子的干种子,在高空飘游８ｈ后回收种植,对其植物学特性及进行分析研究。结果 种子发芽率比对照低５．２％,发芽热比对照高２１．１％。ＳＰ１代植株高度比对照低６．８ｃｍ,旗叶面积比对照高２５％,穗重比对照高１５．９％;ＳＰ３代大穗型株系性状相对稳定,其中ＳＰ３－２的种子蛋白质和脂肪含量均高于对照经高空气球搭载后的ＳＰＥ－３５     

Use of a Calibration Balloon Tube to Size the Pouch and Channel in Gastroplasty Procedures

Gastroplasty is currently one of the most common surgical procedures performed on the morbidly obese for weight loss. An adequate result can be assured only if the pouch that is created is less than 30 ml and the channel that connects that pouch to the distal stomach is approximately 1 cm in diameter. The current method to size the pouch is to occlude the esophagus and the outlet of the pouch and to measure with a manometer through a naso-gastric tube. We contend this method is both time consuming and adds to the potential of complications. Through the use of a calibration balloon tube the size of the pouch can be quickly and safely estimated. It can also be used to size the channel between the pouch and the distal stomach and check for leaks. The technique of how this tube has been used over the past 6 years is described. By the use of a calibration balloon tube, three problem areas in gastric stapling surgery for morbid obesity are avoided, namely: inappropriate pouch size, inappropriate channel size and postoperative leaks.     

桔梗扦插繁殖试验

对桔梗进行扦插繁殖试验，结果看出：用茎基部作插条的成活率最高（５２．２３％），茎梢部作插条成活率很低（１．１１％）；用激素处理可大大提高插条的成活率，其中ＮＡＡ（萘乙酸）１００ｐｐｍ处理３ｈ最显著，比对照组提高２３．３３％；扦插基质湿度过大，会引起插条腐烂。     

Histological findings after angioplasty using conventional balloon, radiofrequency thermal balloon, and stent for experimental aortic coarctation

Abstract Background : Use of balloon angioplasty or stent implantation has been reported to be effective in relieving coarctation of the aorta. However, restenosis frequently occurs after balloon angioplasty for native aortic coarctation in small infants, and sometimes develops after stent implantation because of vessel growth. The causes of restenosis remain uncertain. The purpose of this study was to assess the histologic differences in vascular responses to angioplasty using conventional balloon, radiofrequency thermal balloon (RFTB), or stent for experimental aortic coarctation. Methods : The authors surgically created an aortic coarctation model using 14 puppies. Angioplasty using conventional balloon, RFTB, or stent was performed 1 month after the initial operation. At the acute or chronic phase after angioplasty, the animals were killed and histologic studies were performed. Results : More vascular injuries were noted in the specimens from animals undergoing conventional angioplasty than in those with RFTB or stent. However, neointimal hyperplasia was seen more often after RFTB or stent because of the proliferation of smooth muscle cells from the tunica media, caused by secretion of growth factors. Apoptosis reached a peak 1?2 weeks after angioplasty, regardless of the type of intervention. Conclusions : The authors conclude that angioplasty with RFTB or stent can provide relatively small injuries in the vessel wall for aortic coarctation, but care must be taken to prevent restenosis caused by intimal hyperplasia, because neointima hyperplasia is more frequent after RFTB or stent.     

球瓣比和球囊长度与儿童经皮球囊肺动脉瓣成形术疗效的关系

目的通过分析不同的球瓣比和球囊长度对儿童经皮球囊肺动脉瓣成形术(PBPV)近期及中远期疗效的影响，探讨最适宜的球瓣比和球囊长度。 方法1987~2005年山东省立医院儿科诊治119例肺动脉瓣狭窄患儿，使用不同球瓣比和长度的扩张球囊行PBPV术，扩张前后测量右室与肺动脉间的峰值压力阶差，并行左侧位右室造影，测量瓣环大小并观察有无右室流出道激惹。术前、术后定期行经胸超声心动图检查，估测最大跨肺动脉瓣压力阶差，并观察肺动脉瓣形态及其反流情况。 结果超大球囊法行PBPV术后，患儿的近期及中远期跨肺动脉瓣压差持续下降，且压差下降率不随球瓣比的增加而增大；术后未发现有肺动脉瓣再狭窄者，所有患儿均有不同程度的肺动脉瓣反流，且反流的程度随时间的延长而加重，并与球瓣比成正相关。对于年龄较小(≤6岁)的儿童，球瓣比大且长度≥40mm的球囊较易引起右室流出道痉挛及三尖瓣反流。中远期三尖瓣反流的发生可能间接继发于肺动脉瓣反流所引起的右室容量负荷过重。 结论PBPV治疗肺动脉瓣狭窄，最佳的球瓣比为1.0~1.2，疗效满意且并发症少；6岁以下儿童宜使用长度<40mm的球囊，可减少右室流出道痉挛及近期三尖瓣反流的发生。     

布-加综合征腔内成型术治疗体会

目的 :评价单球囊导管扩张 +内支架置放腔内成型治疗膜性及局限性布 加综合征 ,以探讨此术式的优缺点及适应证。方法 :采用下腔静脉单球囊导管扩张及内支架置放的手术方法对 8例膜性及局限性布 加综合征进行治疗。随访时间 10个月～ 2a。结果 :8例患者均一次性穿刺、扩张成功。阻塞段被扩张至 12mm～ 2 0mm ,行内支架置放腔内成型术后 ,阻塞下方下腔静脉压力从平均 (32± 4 .7)cmH2 O下降至 (19± 3.4 )cmH2 O。随访期内全部患者无复发。结论 :经腔内成型术对于短段闭塞型及重度下腔静脉狭窄型有良好疗效。     

双球囊导管用于瘢痕子宫足月引产的临床观察

目的 探讨瘢痕子宫使用双球囊导管促宫颈成熟及引产后阴道分娩的安全性和有效性.方法 以2016年1月—2018年7月本院收治有阴道分娩条件和意愿的59例宫颈未成熟的足月瘢痕子宫作为研究组,以同期分娩的67例足月非瘢痕子宫宫颈未成熟的为对照组,两组均使用双球囊导管促宫颈成熟及引产,比较两组的母婴结局.结果 研究组孕产妇的年...     

血红素氧合酶-1对大鼠颈动脉球囊损伤后内膜增殖的影响

目的:观察血红素氧合酶-1(HO-1)对大鼠颈动脉球囊损伤后内膜增殖的影响。方法:健康雄性Wistar大鼠40只,随机分为4组(每组10例)。A组:正常对照组;B组:球囊损伤对照组;C组:氯血红素干预组;D组:原卟啉锌(ZnPP)干预组。A组行假手术(分离颈总动脉,但不损伤内膜),B、C、D组按文献方法制作颈动脉内膜损伤模型。B、C、D组于球囊损伤前3d至术后14d,分别给予0.9%氯化钠溶液1ml/只、氯血红素15mg/kg、ZnPP40μmol/kg隔日1次腹腔注射。球囊损伤后第14天处死大鼠,分离颈总动脉,行苏木精-伊红染色,观察颈动脉内膜增殖情况;RT-PCR及Western blotting分析颈动脉组织HO-1mRNA及蛋白质表达。结果:A组颈动脉内膜无增厚,颈动脉组织无HO-1表达;B组和D组颈动脉内膜明显增厚,D组颈动脉组织无明显HO-1表达,而B组颈动脉组织仅有少量HO-1表达;C组颈动脉内膜增厚明显减轻,HO-1表达量显著增高,与B组和D组比较差异有统计学意义(P<0.01)。结论:氯血红素显著抑制颈动脉球囊损伤后新生内膜的形成,提示HO-1在动脉损伤诱导的新生内膜形成中具有保护作用。     

新型双球囊导管经皮选择性隔离肝脏灌注化疗的效果研究

目的探讨自制双球囊导管经皮选择性隔离肝脏灌注化疗的隔离效果。方法12只成年猪随机分为两组：HAI组6头,进行常规经肝动脉灌注化疗;PSIHP组6头,利用自制双球囊导管经介入放射学方法行经皮选择性隔离肝脏灌注化疗结合血液灌流。化疗药物选用5-FU,分别检测肝静脉及外周血液中的血药浓度峰值,了解球囊隔离肝脏效果。结果HAI组肝静脉血和外周静脉血浓度峰值分别为4658.420±433.204mg/L和1676.140±226.933mg/L,PSIHP组为5321.711±517.318mg/L和65.735±6.425mg/L。两组外周静脉血浓度峰值有显著性差异（P〈0.01）。结论自制新型双球囊导管能有效隔离肝脏,是一种理想的隔离肝脏灌注化疗的球囊导管。     

赤芍对内膜增殖机制中丝裂素活化蛋白激酶信号通路的影响探讨

目的:观察赤芍对家兔颈动脉血管成形术后内膜增殖及丝裂素活化蛋白激酶磷酸酶-1(MKP-1)mRNA表达的影响。方法:新西兰白兔随机分为对照及赤芍组,各组均饲喂高脂饲料(普通饲料 2%胆固醇 5%猪油)。赤芍组按剂量给予赤芍提取物:高剂量组(相当于生药3g.kg-1.d-1)、中剂量组(相当于生药2g.kg-1.d-1)、低剂量组(相当于生药1g.kg-1.d-1)。血管成形术后,取材血管行RT-PCR检测MKP-1mRNA表达、形态学测定、免疫组化染色。结果:与对照组比较,增生内膜面积、增生内膜面积/中膜面积显著减少(P<0.05或P<0.01);MKP-1mRNA表达显著增高(P<0.05或P<0.01)。结论:赤芍增加高脂喂养兔颈动脉球囊损伤后MKP-1mRNA表达,减轻内膜增殖程度。表明内膜增殖程度的减低与赤芍阻断MAPK信号通路有关。