Dietary coral calcium and zeolite protects bone in a mouse model for postmenopausal bone loss

In patients diagnosed with osteoporosis, calcium is lost from bones making them weaker and easily susceptible to fractures. Supplementation of calcium is highly recommended for such conditions. However, the source of calcium plays an important role in the amount of calcium that is assimilated into bone. We hypothesize that naturally occurring coral calcium and zeolite may prevent ovariectomy-induced bone loss. We have measured bone loss in ovariectomized mice supplemented with coral calcium and Zeolite. Female C57BL/6 mice were either sham-operated or ovariectomized and fed diets containing coral calcium or zeolite for 6 months. Serum was analyzed for bone biochemical markers and cytokines. Bones were analyzed using dual x-ray absorbtiometry, peripheral quantitative computed tomography, and micro–computed tomography densitometry. In the distal femoral metaphysis, total bone and cortical bone mass was restored and the endocortical surface was significantly decreased in coral calcium and zeolite fed ovariectomized (OVX) mice. Trabecular number and the ratio of bone volume to total volume was higher in OVX mice after coral calcium and zeolite feeding, while trabecular separation decreased in the different treatment OVX groups. Coral calcium protected bone to a lesser extent in the proximal tibia and lumbar vertebrae. Overall, coral calcium and zeolite may protect postmenopausal bone loss.     

珊瑚人工骨的实验研究

研究珊瑚人工骨在成骨过程中所起作用、其生物降解与新骨形成的关系。方法选用６只杂交狗，将珊瑚人工骨植入下颌下缘，以对侧作为对照，采用Ｘ线片、扫描电镜及能谱分析进行观察。结果术后第四、第五个月，实验侧下缘有纤维钙结构和新骨形成，很少有珊瑚人工骨残留，能谱分析钙盐含量很高。     

骨髓基质干细胞与珊瑚复合物体内回植时间的研究

目的　探索骨髓基质干细胞和珊瑚复合物的体内回植时间。方法　骨髓基质干细胞经成骨诱导分化后与珊瑚复合 ,体外培养 1、3、5、7d时行相差显镜镜、扫描电镜观察 ,并行裸鼠皮下回植。结果　 3d时 ,骨髓基质干细胞与珊瑚达到较好的复合状态 ,皮下回植有新骨形成。结论骨髓基质干细胞与珊瑚复合后体外培养 3d时就可以体内回植。     

珊瑚人工骨复合游离骨膜瓣的肌内植入试验

珊瑚人工骨复合游离骨膜植入兔腓肠肌内，所有试样均有新生骨骼和血管组织生成，３周时新骨量占试样的１３．３±７．０，６周时为２２．３±７．０％，新骨于植入５天后开始发生，并持续６周以上。而单纯人工骨或骨膜肌内植入均无新骨产生。全部植入物未发现炎症和排斥反应。     

海南澄黄滨珊瑚作为人工代用骨的预实验研究

本研究的目的是评估和比较种植海南澄黄滨珊瑚(Hainan Coral, Porites Iutea; HNC)和骨基质明胶(Bone Matrix Gelatin; BMG)作为人工代用骨复合种植的实验动物可行性论证。采用实验兔下颌骨缺损种植作为实验模型,设HNC、BMG、HNC—BMG实验组和下颌骨折愈合、自身颌骨移植为对照组,进行喂养观察,X线摄片和组织学观察各项评估。珊瑚的生物相容性得到证实,复合种植不仅互补了材料自身的缺点而且获得了最好的实验效果。     

鹿角珊瑚骨与自体红骨髓复合体的临床应用

目的:探讨采用天然材料结合自体红骨髓移植代替人体骨组织对四肢骨缺损及骨不连的修复作用。方法:采用海洋天然鹿角珊瑚与自体红骨髓混合植入治疗四肢骨缺损及创伤性骨不连接,术后采用X线片、光镜、电镜观察结果。结果:本组稿31例患者中30例骨性愈合,1例因术后感染改用其它方法治疗,骨愈合时间8～14个月。随访10个月～7年。结论:鹿角珊瑚骨理化性质稳定,微细结构与人体骨类同,具有良好生物相溶性和降解性,是红骨髓的良好载体与愈合的框架。以珊瑚骨本身的微细结构为载体吸附的自体红骨髓是唯一含有丰富的定向性和诱导性骨祖细胞的组织,且骨髓间叶细胞诱导成骨活性最强,二者结合的植入物是一种理想的人体骨替代材料。     

黄芪注射液足三里穴位注射治疗银环蛇咬伤致呼吸衰竭临床研究

目的 观察黄芪注射液足三里穴位注射对银环蛇咬伤引起呼吸衰竭的临床疗效.方法 将患者60例随机分为治疗组和对照组,均给予常规治疗,治疗组加用黄芪注射液双侧足三里穴位注射,治疗24 h后比较两组动脉血氧分压（PaO2）、二氧化碳分压（PaCO2）、pH值,5d后比较两组疗效,所有患者治疗结束后比较两组疗程及使用呼吸机时间.结果 治疗后两组PaO2、PaCO2水平差异有统计学意义（P＜0.05）,pH值相当（P＞0.05）;治疗组临床疗效优于对照组（P＜0.05）;治疗组疗程、呼吸机平均使用时间均明显少于对照组（P＜0.05）.结论 黄芪注射液足三里穴位注射对银环蛇咬伤引起呼吸衰竭有良好疗效,能显著改善通气,减少呼吸机使用时间,缩短疗程.     

重组人骨形成蛋白2-珊瑚复合人工骨修复骨质缺损的实验研究

目的弥补珊瑚无骨诱导活性、成骨能力弱等缺陷。方法将珊瑚与重组人骨形成蛋白２（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｂｏｎｅｍｏｒｐｈｏｇｅｎｅｔｉｃｐｒｏｔｅｉｎ－２，ｒｈＢＭＰ－２）复合，制备出ｒｈＢＭＰ－２－珊瑚复合人工骨。将其植入兔颅骨直径１５ｍｍ的圆形缺损，以单纯珊瑚植入作对照。术后２、６、１２周取材，通过组织学观察和计算机图像分析，评价其骨修复能力。结果ｒｈＢＭＰ－２－珊瑚复合人工骨以传导成骨和诱导成骨双重机制完成骨修复过程，骨修复能力和骨修复效果明显优于单纯的珊瑚。结论此复合人工骨是一种较理想的骨移植替代材料。     

Combination of Coral UTI ScreenTM system,gram-stain and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for diagnosis of urinary tract infections directly from urine samples

This study proposes an algorithm for microbiological diagnosis of urinary tract infections based on screening by luminometry and Gram-stain, followed by identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Positive urine samples detected with the luminometry screening Coral UTI Screen^TM system underwent Gram staining and identification of the causative organism was performed by MALDI-TOF Microflex LT mass spectrometer (Bruker Daltonics, Germany). Subsequently, the results were compared with those of conventional culture identification using WIDER MIC/id system (Francisco Soria Melguizo SA, Spain). Considering the conventional approach as the gold standard, the proposed algorithm presented both a high specificity (98.1%) and a positive likelihood ratio of 37.42. The implementation of this algorithm would allow diagnosis of urinary tract infection in less than an hour in 92.4% of positive samples. This combination of techniques would be useful particularly for patients with severe UTI, pyelonephritis or urinary sepsis.     

The Tropical Brown Alga <Emphasis Type="Italic">Lobophora variegata</Emphasis> (Lamouroux) Womersley: A Prospective Bioindicator for Ag Contamination in Tropical Coastal Waters

Uptake and depuration kinetics of dissolved silver were determined in the brown alga Lobophora variegata, using radiotracer techniques. Results indicate that this widely distributed alga could be a useful bioindicator species for surveying silver contamination in tropical environments. Indeed, results showed that the alga readily concentrates silver (algal concentration of silver was 7,000 times higher than in water after a 28-day exposure) and retains it efficiently within its tissues (biological half-life: 72 ± 4 days).