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51.
PCBP1:一种在多水平上参与基因表达调控的蛋白因子 总被引:1,自引:0,他引:1
多聚胞嘧啶结合蛋白1 (poly C binding protein-1,PCBP1),属于RNA结合蛋白亚家族,因其具有同RNA的多聚胞嘧啶(poly C)区域特异的结合活性而得名.目前该亚家族共有5个成员:hnRNP K(heterogeneous nuclear ribonucleoprotein K)、PCBP1、PCBP2、PCBP3和PCBP4.PCBP1蛋白在多个水平参与基因的表达调控,如转录、mRNA加工、mRNA的转运、mRNA稳定以及翻译水平调控等.本文对PCBP1基因的结构和生物学功能进行了综述. 相似文献
52.
细胞凋亡是在一定的生理或病理条件下。由基因控制的细胞自我毁灭过程。凋亡异常与许多疾病(尤其是肿瘤)的形成及发展进程密切相关。细胞凋亡调控机制研究证明。多种基因(如P53、C-myc、Bcl-2、Bax等)参与了细胞凋亡的调控过程。TFAR19基因是由我国科研人员最近新发现的另一重要的凋亡相关基因,笔者主要就其结构特点、性质功能、临床意义作一概述。 相似文献
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In this report, we show that in the human astroglioma cell line D54-MG, both interleukin-1 (IL-1β) and tumor necrosis factor-alpha (TNF-α) enhance C3 gene expression in a time- and dose-dependent manner. Kinetic analysis demonstrates that after 96 h, C3 mRNA levels increase approximately 30-fold and 20-fold in response to IL-1β or TNF-α, respectively. C3 protein production increases proportionally, reaching levels 36-fold and 18-fold higher than untreated controls upon exposure to IL-1β or TNF-α, respectively. D54-MG cells require a minimal 1 h exposure to IL-1β in order to enhance C3 gene expression significantly, while 4 to 8 h are required for TNF-α. Simultaneous treatment of D54-MG cells with IL-1β and interferon-gamma (IFN-γ) resulted in an additive increase in both C3 mRNA and protein expression, a finding not seen with the combination of TNF-α and IFN-γ. Primary rat astrocytes also express increased C3 mRNA levels after 48 h in response to IL-1β (5.3-fold increase) and TNF-α (7-fold increase), while an additive effect was observed upon simultaneous treatment with both IL-1β and IFN-γ. In the central nervous system (CNS), endogenous complement and cytokine production by astrocytes, and enhancement by IFN-γ, a product of activated T cells often seen in the CNS in neural autoimmune disease, may contribute to the pathogenesis of inflammatory demyelinating diseases such as multiple sclerosis. 相似文献
55.
J. N. Laverri re J. L. Richard A. Morin N. Buisson A. Tixier-Vidal W. B. Huttner D. Gourdji 《Molecular and cellular endocrinology》1991,80(1-3):41-51
Secretogranin I (SgI; chromogranin B) belongs to a class of acidic tyrosine-sulfated secretory proteins believed to play a role in the secretory process of endocrine cells. Our aim here was to compare the levels of SgI mRNA to that of prolactin (PRL) and growth hormone (GH), using rat pituitary cell lines. As far as the constitutive expression is concerned, we found a positive correlation between SgI mRNA and PRL mRNA levels. However, the neuropeptide TRH (50 nM) inhibited the accumulation of SgI mRNA in GH3B6 cells whereas, as expected, it induced a rapid and sustained increase in PRL mRNA accumulation. By contrast, 17β-estradiol (1 nM) stimulated the accumulation of both SgI and PRL mRNAs, with the same EC50 (18–59 pM). Reciprocally, treatment with dexamethasone (100 nM) reduced the level of SgI and PRL mRNAs to 23% and 29% of control, respectively, but led to a 2.1-fold increase in the GH mRNA level. Altogether, the present work shows that SgI gene expression is subject to multiple hormonal regulations and occasionally parallels the regulation of the PRL gene but never that of the GH gene, under the conditions tested. 相似文献
56.
人重组C3片段通过IL-2自分泌效应对CTLL-2细胞产生促增殖作用 总被引:1,自引:0,他引:1
目的研究补体C3片段的体外生物学活性及其作用机理,进一步探讨C3片段与免疫细胞的关联。方法利用重组DNA技术表达纯化C3活性片段(命名为C33),观察其对IL-2依赖性的小鼠杀伤性T细胞株CTLL-2细胞的促增殖效应,并通过抗体封闭途径和分子杂交技术研究C33作用的机理。结果发现C33蛋白对CTLL-2细胞具有明显的促增殖作用,并呈剂量依赖关系;这一作用能够部分地被抗小鼠CD11b抗体所封闭,能够完全被抗小鼠IL-2抗体所封闭;分子杂交显示C33蛋白能够明显刺激CTLL-2细胞的IL-2mRNA表达。结论人重组C3片段C33可通过IL-2的自分泌效应对CTLL细胞产生促增殖作用,补体受体CR3参与这一作用。 相似文献
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58.
Brigitte Schiller Chun He David J. Salant Alice Lim Jessy J. Alexander Richard J. Quigg 《The Journal of experimental medicine》1998,188(7):1353-1358
Crry (complement receptor 1–related protein/gene y) is a key cellular complement regulator in rodents. It is also present in Fx1A, the renal tubular preparation used to immunize rats to induce active Heymann nephritis (HN), a model of membranous nephropathy. We hypothesized that rats immunized with anti-Fx1A develop autoantibodies (auto-Abs) to Crry as well as to the megalin-containing HN antigenic complex, and that anti-Crry Abs promote the development of injury in HN by neutralizing the complement regulatory activity of Crry. Rats immunized with Fx1A lacking Crry remained free of proteinuria and glomerular deposits of C3 during a 10-wk follow-up despite typical granular immunoglobulin (Ig)G deposits in glomeruli. Anti-Fx1A auto-Abs were present in their sera at levels that were not different from sera pooled from proteinuric rats with HN induced with nephritogenic Fx1A. Passive administration of sheep anti-Crry Abs to rats immunized with Crry-deficient Fx1A led to proteinuria and glomerular C3 deposition, which were not seen in such rats injected with preimmune IgG, nor in rats with collagen-induced arthritis injected with anti-Crry IgG. To directly examine the role of Crry in HN, rats were immunized with Crry-deficient Fx1A reconstituted with rCrry. This led to typical HN, with 8 out of 15 rats developing proteinuria within 14 wk. Moreover, the extent of glomerular C3 deposition correlated with proteinuria, and anti-Crry Abs were present in glomerular eluates. Thus, Crry is a key nephritogenic immunogen in Fx1A. Formation of neutralizing auto-Abs to Crry impairs its function, leading to unrestricted complement activation by Abs reactive with the HN antigenic complex on the epithelial cell surface. 相似文献
59.
大鼠烧伤后急性期蛋白mRNA的表达 总被引:2,自引:0,他引:2
分析了不同烧伤程度大鼠肝、脾、肾和骨骼骨内4种急性期蛋白,即a1-酸性糖蛋白、a1-抗胰蛋白酶、白蛋白和转铁蛋白(Trf)mRNA水平的动态变化特征,结果表明:(1)肝脏是合成各种APR的主要场所;(2)即使是小面积的烧伤也可以诱发急性期反应。(3)烧伤后血清Alb水平下降是急性期反应的特征,但在mRNA水平上并不下降而是增加。 相似文献
60.