Long-term exocrine function of the pancreas transplant

The long-term exocrine function of fifty-nine pancreaticoduodenal isografts was evaluated in rats for up to one year post transplantation. At one, three, six, nine, and twelve months after transplantation the grafts were cannulated and the exocrine secretion was collected. The volume, protein content, pH, amylase and trypsin concentrations, and electrolyte composition of the secretion were compared with those obtained from the host pancreas in nineteen control rats. Twenty-four hour secretion studies demonstrated normal basal function of the pancreas transplant when compared with that of the host. Pancreozymin stimulation caused an increase in volume, trypsin concentration, and amylase concentration of the graft pancreas secretion that was similar to those seen in the host. These studies indicate that there is normal exocrine secretion of pancreas transplants in the absence of rejection and that denervation of the gland has little direct effect on over-all pancreatic function.     

术后认知功能障碍治疗的新靶向-γ-氨基丁酸A型受体相关药物

背景 术后认知功能障碍(postoperative cognitive dysfunction,POCD)是一种常见的围术期并发症.γ-氨基丁酸A型(γ-aminobutyricacid type A,GABAA)受体作为一种中枢神经抑制性受体,可能对POCD的发生起着关键性作用.目的 综述以GABAA受体为靶点的相关药物对POCD的影响.内容 与GABAA受体相关的3类药物:激动剂、拮抗剂和反向激动剂,对POCD可分别产生诱发、逆转和改善症状的作用. 趋向 对记忆或者认知功能损害具有改善作用的GABAA受体相关药物,对POCD的治疗具有重大意义,将成为今后POCD的研究热点.     

高糖培养下系膜细胞JAK2／STAT3信号转导通路活性变化及与活性氧簇作用的研究

目的：通过观察高糖培养条件下大鼠肾小球系膜细胞P-STAT3的表达变化,探讨糖尿病状态下JAK2／STAT3途径活性的变化及该通路与活性氧簇（ROS之间的相互作用。方法：用传代培养的大鼠肾小球系膜细胞同步化后分组：（1）正常糖浓度组（含5．5mmol／L）,高糖浓度组（25mmol／L）,甘露醇组（5．5mmot／L糖＋19．5mmol甘露醇）,正常糖＋AG-490（浓度10／μmol／L）组,高糖＋AG-490（浓度10μmol／L）组。继续观察培养后用Westem Blot及细胞免疫化学方法检测系膜细胞STAT3、P-STAT3表达的变化。（2）正常糖浓度组（N）,高糖浓度组（H）,甘露醇组（M）,正常糖＋Apocynin组（N＋A,Apocynin浓度为100μmol／L）,高糖＋Apocynin组（H＋A,Atx）cynin浓度为100μmol／L）,收集上清液,用比色法检测系膜细胞ROS水平。（3）NADPH氧化酶抑制剂Apocynin预处理,分组同（2）,Apocynin提前1h加入,与正常糖或高糖同时培养后,用Westem Blot方法检测系膜细胞P-STAT3表达。结果：（1）高糖培养大鼠肾小球系膜细胞P-STAT3的表达较正常糖浓度组明显升高,甘露醇组与正常糖浓度组相比差异无统计学意义;各组之间STAT3表达差异无统计学意义。（2）高糖条件下,ROS产生明显升高,NADPH氧化酶抑制剂Apocynin可明显降低ROS的产生。（3）高糖条件下,Apocynin经预处理,在正常糖浓度和高糖浓度同时培养48h后,正常糖浓度组和正常糖＋Apocynin组对比P-NTAT3的表达差异无明显区别;高糖十Apocynin组较正常糖浓度组有明显区别,但与高糖组相比明显降低。结论：高糖通过磷酸化方式激活大鼠肾小球系膜细胞JAK2／STAT3信号转导通路;高糖作用下,肾小球系膜细胞ROS产生增加,并具有时间依赖性;高糖状态下ROS可激活肾小球系膜细胞的JAK2／STAL信号传导通路,证明ROS可能参与糖尿病肾病的发生和发展过程。     

The Administration of Panax Ginseng Berry Extract Attenuates High-Fat-Diet-Induced Sarcopenic Obesity in C57BL/6 Mice

Sarcopenia and obesity are serious health problems that are highly related to several metabolic diseases. Sarcopenic obesity, a combined state of sarcopenia and obesity, results in higher risks of metabolic diseases and even mortality than sarcopenia or obesity alone. Therefore, the development of therapeutic agents for sarcopenic obesity is crucial. C57BL/6 mice were fed with a high-fat diet (HFD) for 9 weeks. Then, mice were administered with Panax ginseng berry extract (GBE) for an additional 4 weeks, with continuous HFD intake. GBE significantly decreased the food efficiency ratio, serum lipid and insulin levels, adipose tissue weights, and adipocyte size. It significantly increased the grip strength, muscle masses, and myofiber cross-sectional area. It deactivated the protein kinase C (PKC) theta and zeta, resulting in activation of the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway, which is known to regulate muscle synthesis and degradation. Furthermore, it inhibited the production of inflammatory cytokines in the muscle tissue. GBE attenuated both obesity and sarcopenia. Thus, GBE is a potential agent to prevent or treat sarcopenic obesity.     

Manufacturing of a Magnetic Composite Flexible Filament and Optimization of a 3D Printed Wideband Electromagnetic Multilayer Absorber in X-Ku Frequency Bands

With the multiplication of electronic devices in our daily life, there is a need for tailored wideband electromagnetic (EM) absorbers that could be conformed on any type of surface-like antennas for interference attenuation or military vehicles for stealth applications. In this study, a wideband flexible flat electromagnetic absorber compatible with additive manufacturing has been studied in the X-Ku frequency bands. A multilayer structure has been optimized using a genetic algorithm (GA), adapting the restrictions of additive manufacturing and exploiting the EM properties of loaded and non-loaded filaments, of which the elaboration is described. After optimization, a bi-material multilayer absorber with a thickness of 4.1 mm has been designed to provide a reflectivity below −12 dB between 8 and 18 GHz. Finally, the designed multilayer structure was 3D-printed and measured in an anechoic chamber, achieving −11.8 dB between 7 and 18 GHz. Thus, the development of dedicated materials has demonstrated the strong potential of additive technologies for the manufacturing of thin wideband flexible EM absorbers.     

脂褐素成分A2E诱导人视网膜色素上皮细胞自噬及损伤的研究

目的 探讨N-亚视黄基-N-视黄基-乙醇胺(A2 E)能否诱导人视网膜色素上皮细胞(ARPE-19细胞)的自噬及损伤反应,并从自噬的角度探索其与年龄相关性黄斑变性(AMD)发病相关的分子机制.方法 CCK-8法筛选A2E作用于ARPE-19细胞的最佳浓度用于后续实验.采用多重细胞因子检测技术检测A2E作用于ARPE-1...     

结核病的体液免疫诊断研究(V.PPA—ELISA检测胸、腹水结核抗体)

以PPD为抗原,建立了检测人体胸、腹水中抗结核菌抗体的PPA-ELISA,对方法的特异性、重复性和最适检测条件进行了探讨。63份患者胸、腹水标本的检测结果显示,28例结核性胸膜炎(腹膜炎)患者胸、腹水的特异性抗体水平非常明显地高于35例对照组患者(P<0.001)。本法简便快速且灵敏,可作为结核性胸膜炎(腹膜炎)的辅助诊断方法;其动态观察还可为疗效考核提供依据,作者等建议加以推广应用。     

线粒体去乙酰化蛋白SIRT3在噪声诱导隐匿性听力损失中的作用

目的 探讨线粒体去乙酰化蛋白(sirtuin-3,SIRT3)在噪声性隐性听力损失(noise-induced hidden hearing loss, NIHHL)中的作用。方法 将注射过SIRT3抑制剂[3-(1H-1,2,3-triazol-4-yl) pyridine,3-TYP]与未经处理的C57BL/6J小鼠分别于100 dB白噪声下暴露2 h,并在噪声暴露 (noise exposure, NE) 后1 d和14 d进行测听。利用免疫荧光染色观察内毛细胞 (inner hair cell, IHCs)带状突触(ribbon synapses, RS)的变化。冰冻切片下,4-羟基壬烯醛 (4-hydroxynonenal, 4-HNE)染色观察耳蜗内活性氧(reactive oxygen species, ROS) 的累积。结果 与对照组相比,噪声组及3-TYP组小鼠均在噪声后出现短暂性听力阈值的升高,ROS的累积及带状突触的丢失。与噪声组小鼠相比,3-TYP组小鼠在噪声后第14天听力未恢复正常,带状突触丢失明显,且伴有ROS的显著累积。结论 NE诱发了内耳的氧化应激反应,SIRT3可能通过抵抗氧化应激作用来保护耳蜗带状突触及听功能。     

小儿肛周脓肿56例治疗分析

目的：探讨小儿肛周脓肿的治疗效果。方法该院肛肠科于2012年6月—2014年6月期间对该院收治的56例小儿肛周脓肿患者进行一期根治术进行治疗,设为实验组,另外的56例患者进行切开引流术治疗小儿肛周脓肿,设为对照组,手术后观察患者的治疗效果。结果56例小儿肛周脓肿患者通过一期根治术治疗（实验组）,全部患者均有好转,治愈率为98.2%,进行切开引流术（对照组）的治愈率为87.5%,两组患儿的治愈率的比较,差异有统计学意义（P＜0.05）;实验组在复发率方面明显少于对照组,差异有统计学意义（P＜0.05）;实验组在切口愈合时间上明显短于对照组,差异有统计学意义（P＜0.05）。结论对小儿肛周脓肿进行一期根治术的治疗效果明显,无并发症,具有重要的临床意义,值得推广应用。     

VEGF受体3亲和肽亲和特性研究

目的通过测定人源VEGF受体3(VEGFR3)亲和肽(WHGSLKQNLWWY)及VEGF-D与VEGFR3的功能性亲和常数,鉴定亲和肽的亲和特性.方法通过戊二醛耦联法包被多肽,确定最佳多肽包被浓度,最佳多肽与VEGFR3结合反应时间以及包被系数后,采用非竞争性ELISA固项法,得到多肽及VEGF-D与VEGFR3的结合反应曲线,计算多肽及VEGF-D与VEGFR3的亲和常数.结果多肽及VEGF-D与VEGFR3的功能性亲和常数分别为(1.42±1.45)×107mol/L-1和(1.03±1.16)×108mol/L-1.结论该多肽与VEGFR3亲合能力较强,为今后开发以该多肽为基础的靶向治疗药物提供了理论依据.