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991.
黄芪总黄酮对急性心肌梗死大鼠心脏血流动力学及心肌细胞钙电流的作用 总被引:2,自引:0,他引:2
目的探讨黄芪总黄酮(TFA)对急性心肌梗死(AMI)大鼠心脏血流动力学及心肌细胞钙电流的作用。方法大鼠开胸左前降支结扎造成AMI,开胸前5min实验组大鼠舌静脉注射剂量为(20mg/kg)TFA溶液50μl,并设正常对照组,AMI组及TFA实验组,后行血流动力学检查;酶解分离心室肌细胞,采用全细胞膜片钳记录技术记录左前降支供血区心外膜细胞的L型Ca^2+电流(L—Ica)的作用。结果①应用TFA(20mg/kg)后与AMI组比较,血流动力学指标明显改善(P〈0.05,n=7)。与正常对照组相比差异无统计学意义(P〉0.05,n=7)。②应用TFA(20mg/kg)后与AMI对照组比较,L-型钙电流(L—ICa)由给药前的(0.31±1.09)nA增加到给药后的(0.49±0.89)nA(P〈0.01,n=7)。结论TFA可以明显改善AMI大鼠心脏血流动力学,增加心室肌细胞L-型钙电流的幅值。 相似文献
992.
The properties of a specific adenosine nucleotide translocase from rat and pigeon heart mitochondria are similar to those of the system from liver. In particular, the Km for external ADP is very low, i.e. about 7 μm. The maximum activity of the translocase system, which was measured by following the exchange of adenine nucleotides across the mitochondrial membrane, or the Km of the system for ADP or ATP were not affected by Ca2+ concentration in the range 0.001 to 10 μm. Consequently the concentration change in sarcoplasmic Ca2+ that modifies the activities of several key metabolic enzymes plus the myofibrillar ATPase is unlikely to affect the activity of the translocase. However, in confirmation of previous work, higher concentrations of Ca2+ (up to 400 μm) increased considerably the exchange of the adenine nucleotides. There is some recent evidence that heart muscle mitochondria possess high affinity binding sites for Ca2+ in the mitochondrial membrane, which bind up to 300 nmol Ca2+ per g of heart muscle. It is emphasized that, if the adenine nucleotide translocase and the high affinity binding sites for Ca2+ are adjacent (or identical), the effects of the higher concentrations of Ca2+ on the translocase could be of considerable importance in control of the rate of translocation in heart muscle. 相似文献
993.
本实验观察了家兔创伤后胃粘膜损伤情况及川芎嗪的防治效应。发现动物创伤后目粘膜损伤严重.出现应激性溃疡.同时血浆和胃粘膜组织中脂质过氧化物商二醇含量增加,且胃粘膜细胞有明显的钙积聚。而应用川芎嗪防治的动物.脂质过氧化反应被明显地抑制,钙超载现象显著减轻.胃粘膜损伤轻微。提示,应激性溃疡的发生与自由基反应和细胞内钙超载有关,川芎嗪对应激性溃疡具有防治作用。 相似文献
994.
The effect of orlistat on body weight and coronary heart disease risk profile in obese patients: the Swedish Multimorbidity Study 总被引:1,自引:0,他引:1
Lindgärde F 《Journal of internal medicine》2000,248(3):245-254
OBJECTIVE: To assess the effect of orlistat on body weight and cardiovascular risk amongst obese patients at high coronary risk. DESIGN: After screening, patients entered a two-week single-blind placebo lead-in period, during which they followed a mildly hypocaloric diet, before being randomized to double-blind treatment with either orlistat 120 mg or placebo three times daily, in conjunction with dietary intervention for 1 years. SETTING: The study was conducted at 33 primary care centres in Sweden. SUBJECTS: A total of 382 obese adults (body mass index 28-38 kg m-2) with type 2 diabetes, hypercholesterolaemia and/or hypertension were recruited, of whom 376 were randomized to orlistat (n = 190) or placebo (n = 186). MAIN OUTCOME MEASURES: Change in body weight, waist and hip circumferences, blood pressure, serum lipid profile, fasting glucose and HbA1c. RESULTS: After 1 years, mean weight loss was significantly greater with orlistat compared with placebo (5.9% vs. 4.6%; P < 0.05). Moreover, significantly more orlistat-treated patients than placebo recipients maintained weight loss of > or = 5% (54.2% vs. 40.9%; P < 0.001). Orlistat was also associated with significantly greater improvements than placebo in total serum cholesterol (- 3.3% vs. -0.5%; P < 0.05), LDL-cholesterol (- 7.0% vs. -1.1%; P < 0.05), fasting glucose (5.1% vs. -0.1%; P < 0.01) and HbA1c (- 2.7% vs. -0.5%; P < 0.05). Similar results were reported for the subgroup of patients with type 2 diabetes. Orlistat was well tolerated. CONCLUSIONS: Treatment with orlistat in conjunction with diet promotes significantly greater weight loss and cardiovascular risk factor reduction than diet alone amongst obese patients at high risk of future coronary events. 相似文献
995.
Andre Gervais Ulrich Busch Jeanie M. Wood Arnold Schwartz 《Journal of molecular and cellular cardiology》1978,10(11):1003-1015
The effects of toxic doses of ouabain on two parameters of mitochondrial activity, oxidative phosphorylation and calcium uptake were examined. Ouabain was injected intraperitoneally into guinea-pigs until signs of severe intoxication appeared. State 3 oxygen consumption (QO2, State 3, in natom oxygen/mg/min) of isolated heart mitochondria was 314 ± 16 and 281 ± 16 (glutamate-malate) for treated and control group, respectively; 225 ± 21 and 207 ± 23 (pyruvate-malate), and 251 ± 12 and 230 ± 13 (succinate), respectively. The rate of calcium uptake was 411 nmol Ca2+/min/mg for treated and 329.6 nmol Ca2+/min/mg for control. The rate of calcium release was the same in control and treated groups.The data suggest that increases of respiration and calcium uptake in vitro, if they reflect similar increases in vivo, may contribute to digitalis intoxication by intracellular redistribution of calcium. 相似文献
996.
T. McLaughlin F. Abbasi C. Lamendola G. Yee S. Carter S.W. Cushman 《Nutrition, metabolism, and cardiovascular diseases : NMCD》2019,29(1):62-68
Background and Aims
Overweight and obesity increase risk for diabetes and cardiovascular disease, largely through development of insulin resistance. Benefits of dietary weight loss are documented for obese individuals with insulin resistance. Similar benefits have not been shown in overweight individuals. We sought to quantify whether dietary weight loss improves metabolic risk profile in overweight insulin-resistant individuals, and evaluated potential mediators between weight loss and metabolic response.Methods and Results
Healthy volunteers with BMI 25–29.9 kg/m2 underwent detailed metabolic phenotyping including insulin-mediated-glucose disposal, fasting/daylong glucose, insulin, triglycerides, FFA, and cholesterol. Subcutaneous fat biopsies were performed for measurement of adipose cell size. After 14 weeks of hypocaloric diet and 2 weeks of weight maintenance, cardiometabolic measures and biopsies were repeated. Changes in weight, % body fat, waist circumference, adipose cell size and FFA were evaluated as predictors of change in insulin resistance.Weight loss (4.3 kg) yielded significant improvements in insulin resistance and all cardiovascular risk markers except glucose, HDL-C, and LDL-C. Improvement in insulin sensitivity was greater among those with <2 vs >2 cardiovascular risk factors at baseline. Decrease in adipose cell size and waist circumference, but not weight or body fat, independently predicted improvement in insulin resistance.Conclusions
Weight loss yields metabolic health benefits in insulin-resistant overweight adults, even in the absence of classic cardiovascular risk factors. Weight loss-related improvement in insulin sensitivity may be mediated through changes in adipose cell size and/or central distribution of body fat. The insulin-resistant subgroup of overweight individuals should be identified and targeted for dietary weight loss.Clinical trials identifier
NCT00186459. 相似文献997.
Mitchell L. Ramsey Erin Talbert Daniel Ahn Tanios Bekaii-Saab Niharika Badi P. Mark Bloomston Darwin L. Conwell Zobeida Cruz-Monserrate Mary Dillhoff Matthew R. Farren Alice Hinton Somashekar G. Krishna Gregory B. Lesinski Thomas Mace Andrei Manilchuk Anne Noonan Timothy M. Pawlik Priyani V. Rajasekera Phil A. Hart 《Pancreatology》2019,19(1):80-87
Background
Cachexia is a wasting syndrome characterized by involuntary loss of >5% body weight due to depletion of adipose and skeletal muscle mass. In cancer, the pro-inflammatory cytokine interleukin-6 (IL-6) is considered a mediator of cachexia and a potential biomarker, but the relationship between IL-6, weight loss, and cancer stage is unknown. In this study we sought to evaluate IL-6 as a biomarker of cancer cachexia while accounting for disease progression.Methods
We retrospectively studied 136 subjects with biopsy-proven pancreatic ductal adenocarcinoma (PDAC), considering the high prevalence of cachexia is this population. Clinical data were abstracted from subjects in all cancer stages, and plasma IL-6 levels were measured using a multiplex array and a more sensitive ELISA. Data were evaluated with univariate comparisons, including Kaplan-Meier survival curves, and multivariate Cox survival models.Results
On multiplex, a total of 43 (31.4%) subjects had detectable levels of plasma IL-6, while by ELISA all subjects had detectable IL-6 levels. We found that increased plasma IL-6 levels, defined as detectable for multiplex and greater than median for ELISA, were not associated with weight loss at diagnosis, but rather with the presence of metastasis (p?<?0.001 for multiplex and p?=?0.007 for ELISA). Further, while >5% weight loss was not associated with worse survival, increased plasma IL-6 by either methodology was.Conclusion
Circulating IL-6 levels do not correlate with cachexia (when defined by weight loss), but rather with advanced cancer stage. This suggests that IL-6 may mediate wasting, but should not be considered a diagnostic biomarker for PDAC-induced cachexia. 相似文献998.
José Alberto de Agustín José Juan Gómez de Diego Pedro Marcos-Alberca Patricia Mahía José Luis Rodrigo María Luaces Iván Javier Núñez-Gil Joaquín Ferreiros Ana Bustos Beatriz Cabeza Miguel Ángel García-Fernández Carlos Macaya Leopoldo Pérez de Isla 《Revista espa?ola de cardiología》2018,71(2):105-109
Introduction and objectives
Multidetector computed tomography (MDCT) has been demonstrated as a feasible alternative to invasive coronary angiography (ICA). However, contradictory results have been reported regarding the effect of coronary artery calcium score (CS) on the diagnostic accuracy of MDCT. Our aim was to assess the agreement of MDCT and ICA and to evaluate the influence of CS on this agreement.Methods
We enrolled 266 consecutive patients who underwent evaluation with 64-slice MDCT and ICA. Standard CS software tools were used to calculate the Agatston score. Stenosis was qualitatively classified as mild, moderate, or severe by 1 blinded observer and the results were compared with those of ICA, which was used as the gold standard.Results
The mean age of the patients was 65.4 ± 11.2 years, and 188 patients (70.3%) were men. A total of 484 segments with coronary stenosis ≥ mild were qualitatively evaluated and quantified with MDCT. Noninvasive measurements were concordant with ICA in 402 stenoses (83.05%; Kappa, 0.684), with no significant differences between vessels and with no statistically significant influence of CS on this agreement (OR, 0.93; 95%CI, 0.76-1.09; P = .21). Multidetector computed tomography had high sensitivity, specificity, positive predictive value, and negative predictive value on a per-segment, per-vessel, and per-patient basis.Conclusions
Non-ICA using MDCT showed good agreement with ICA in the qualitative quantification coronary stenosis and CS had no significant impact on this agreement.Full English text available from: www.revespcardiol.org/en 相似文献999.
Coordinated control of connexin 26 and connexin 30 at the regulatory and functional level in the inner ear 下载免费PDF全文
1000.