缺血预处理对犬脊髓损伤及热休克蛋白70表达影响的研究

目的评价缺血预处理对犬脊髓损伤及热休克蛋白70表达的影响。方法41条杂种犬随机分成假手术组6只、预处理组21只、对照组14只。预处理组主动脉阻断6min后开放6min,反复2次,之后阻断35min;对照组主动脉阻断35min。术后进行神经功能评分,检测脊髓组织中热休克蛋白70表达。结果在再灌注后6h、24h预处理组热休克蛋白70于胞质和胞核均有表达,且强于对照组;而且神经功能评分预处理组高于对照组。在再灌注后7d预处理组神经功能评分无明显改变,且仍见热休克蛋白70表达。结论缺血预处理可以增加脊髓的缺血耐受;热休克蛋白70在胞质和胞核中表达可能在缺血耐受中起到一定的作用。     

Bile duct injuries during laparoscopic cholecystectomy: a 1994–2001 audit on 13,718 operations in the area of Rome

Background Bile duct injuries (BDIs) during laparoscopic cholecystectomy (LC) still are reported with greater frequency than during open cholecystectomy (OC).Methods In 1999, a retrospective study evaluating the incidence of BDIs during LC in the area of Rome from 1994 to 1998 (group A) was performed. In addition, a prospective audit was started, ending in December 2001 (group B).Results In group A, 6,419 LCs were performed (222 were converted to OC; 3.4%). In group B, 7,299 LCs were performed (225 were converted to OC; 3.1%). Seventeen BDIs (0.26%) occurred in group A and 16 (0.22%) in group B. Overall, mortality and major morbidity rates were 12.1% and 30.3%, respectively, without significant differences between the two groups.Conclusions The incidence and clinical relevance of BDIs during LC in the area of Rome appeared to be stable over the past 8 years and were not influenced by the use of a prospective audit, as compared with a retrospective survey.(T. Zanarini) Other members that actively participated in this multicenter study are: C. Allegri, Ospedale Cristo Re, Roma; D. Antonellis, Ospedale S. Giuseppe, Marino; N. Basso, Università La Sapienza, Roma; F. Cancrini, Ospedale S. Carlo di Nancy, Roma; G. Cucchiara, Ospedale Fatebenefratelli, Roma; A. Garofalo, Ospedale S. Camillo Forlanini, Roma; A.L. Gaspari, Università Tor Vergata, Roma; M. Lombardi, Ospedale CTO, Roma; G. Massi, Ospedale S. Camillo Forlanini, Roma; R. Marrese, G.B. Grassi, Ospedale S. Filippo Neri, Roma; V. Mazzucato, M. Mulieri, Ospedale Nuovo Regina Margherita, Roma; A. Montori, Università La Sapienza, Roma; A. Moraldi, Ospedale S. Giacomo, Roma; G. Pasquini, Ospedale Civile, Velletri; E. Santoro, Istituto Regina Elena, Roma; F. Scutari, Ospedale S. Spirito, Roma; R. Tersigni, Ospedale S. Camillo Forlanini, Roma; C.E. Vitelli, Ospedale Madre G. Vannini, Roma; F. Zaraca, M. Carboni, Università La Sapienza, Roma     

Spontaneous healing process of a supraspinatus tendon tear in rabbits

Introduction The rotator cuff has a characteristic structure, in that one surface faces articular cartilage and another faces bursa. This structure may produce differences in the healing process between the rotator cuff and other tendons. We investigated the spontaneous healing process of a surgically created supraspinatus tendon tear in rabbits.Materials and methods A transverse, full-thickness tear of the supraspinatus tendon was created and its healing examined.Results A tear of 12 mm was not repaired within 3 weeks. With a tear of 5 mm, reparative tissue gradually encroached into the defect from the bursal side, and the tear united from the bursal side to the articular side by 12 weeks. The healing rates (width of reparative tissue/width of the tendon×100%) were 32.2%, 52.4%, 58.0%, 88.9%, and 93.8% at 1, 2, 3, 6, and 12 weeks, respectively. The reparative tissue had continuity to the epitenon of the bursal side. Immunohistochemical study showed that at week 1, type III collagen was detected in the reparative tissue and the cutting ends, and the expression gradually decreased. On the other hand, the expression of type I collagen in the reparative tissue was weak at week 1 and increased until week 3. PCNA-positive cells were observed in the reparative tissue.Conclusion These results show that the origin of the reparative tissue is the epitenon, and from the bursal side rather than the articular side. This model is very useful for the investigation of the remodeling process of an acute rotator cuff tear.     

Usefulness of both operative cholangiography and conversion to decrease major bile duct injuries during laparoscopic cholecystectomy

Background/Purpose We evaluated the role of operative cholangiography and of conversion to decrease major bile duct injuries.Methods We report 1074 patients who underwent laparoscopic cholecystectomy, out of a total of 1195 patients who underwent laparoscopy, over an 8-year period. The planned laparoscopic operative procedure in all the patients was the standard four-port technique with the operator on the left side of the patient. Operative cholangiography was performed with Olsens pliers.Results We performed 993 (83%) operative cholangiographies; 121 (10.1%) patients were converted from laparoscopic to open cholecystectomy. Despite a prolonged time of dissection, 54 (4.5%) patients were converted because of unclear anatomy of Calots triangle. One hundred and ninety patients suffered acute cholecystitis and, of those, 52 (27.3% of 190 patients) were converted. Fifteen patients showed intraoperative biliary duct stones and they were converted. Seven (0.58%) bile duct injuries (one stricture and six fistulas) are reported.Conclusions The low number of major bile duct injuries reported in our study showed the value of operative cholangiography during laparoscopic cholecystectomy. Moreover, another important factor found to reduce major bile duct injuries was conversion when, despite accurate dissection, the anatomy of Calots triangle remained unclear.Presented at the poster session of the 103rd Congress of The Japan Surgical Society, Sapporo, Hokkaido, Japan, June 2003, and published in abstract form in the Journal of the Japan Surgical Society (2003) 104: 1072–1073 (data-related years 1993–2000).     

Major bile duct injuries after laparoscopic cholecystectomy: A tertiary center experience

Bile duct injury is a severe and potentially life-threatening complication of laparoscopic cholecystectomy. Several series have described a 0.5% to 1.4% incidence of bile duct injuries during laparoscopic cholecystectomy. The aim of this study was to report on an institutional experience with the management of complex bile duct injuries and outcome after surgical repair. Data were collected prospectively from 40 patients with bile duct injuries referred for surgical treatment to our center between April {dy1998} and December 2003. Prior to referral, 35 patients (87.5%) underwent attempts at surgical reconstruction at the primary hospital. In77.5%of the patients, complex typeE1or typeE2BDIwas found. Concomitant with bile duct injury, seven patients had vascular injuries. Roux-en-Y hepaticojejunostomy was carried out in 33 patients. In two patients, Roux-en-Y hepaticojejunostomy and vascular reconstruction were necessary. Five patients, one with primary nondiagnosed Klatskin tumor, required right hepatectomy. Two patients, both with bile duct injuries and vascular damage, died postoperatively. Because of progressive liver insufficiency, one of them was listed for high-urgency liver transplantation but died prior to intervention. At the median follow-up of 589 days, 82.5% of the patients are in excellent general condition. Seven patients have signs of chronic cholangitis. Major bile duct injuries remain a significant cause of morbidity and even death after laparoscopic cholecystectomy. Because they present a considerable surgical challenge, early referral to an experienced hepatobiliary center is recommended.     

Dental Injuries in Intermediate and High School Athletes: A 15-Year Study at Punahou School

OBJECTIVE: To determine the rate, type, and severity of dental injuries recorded for intermediate and high school interscholastic athletic participants. DESIGN AND SETTING: A longitudinal study (1988-2003) of intermediate and high school athletes utilizing the same certified athletic trainers to evaluate and record all injury data. SUBJECTS: Sports participation included 123 teams in 19 female, 18 male, and 2 coed sports. Of 2445 Punahou School intermediate and high school students, on average, 1340 students (623 females, 717 males) annually participated in interscholastic athletics. MEASUREMENTS: Dental injuries were defined as injuries to the jaw, teeth, and oral soft tissue (lip, mouth, cheek, and tongue). Soft tissue injuries requiring physician or dentist referral were recorded. Other soft tissue injuries were treated as skin abrasions and were not recorded. Actual days lost from activity were recorded. The estimated injury rate was determined (injuries/1000 athlete-sessions). Mouth-guard use was recorded. RESULTS: During the 15-year study, 19 492 injuries were reported, with 56 (0.2%) recorded as dental injuries (23 tooth, 20 jaw, and 13 soft tissue). Injury rates were highest for girls' wrestling (0.243, confidence interval = 0-2.3), boys' judo (0.189, confidence interval = 0-3.6), and boys' soccer (0.127, confidence interval = 0.4-1.4). The football injury rate was 0.029 (confidence interval = 0.04-0.29), with no tooth injuries. CONCLUSIONS: The incidence and injury rate of dental injuries was extremely low for all reported sports. A universal definition of dental injuries must be established to facilitate injury data collection and analysis.     

Medical procedures, complications, and outcomes for patients with spinal cord injury: A multicenter investigation comparing African Americans and whites

OBJECTIVE: To examine the role of race on rehabilitation outcomes for a matched sample of patients with spinal cord injury (SCI). DESIGN: African Americans and whites with SCI were matched based on age group, level and completeness of injury, and sponsor of care to retrospectively analyze the impact of race. SETTING: Eighteen medical centers in the federally sponsored Model Spinal Cord Injury Systems project. PARTICIPANTS: A total of 628 adults with SCI. INTERVENTIONS: Not applicable. MAIN OUTCOME MEASURES: Medical procedures and complications; American Spinal Injury Association motor index, and FIMT instrument scores at admission and discharge; and discharge dispositions. RESULTS: Analysis revealed race-related differences in spinal surgeries, laparotomies, traction during acute care, and method of bladder management at discharge. In most cases, these were explained by cause of injury rather than direct affects of race. No differences were found with regard to medical complications functional outcomes, or discharge disposition. CONCLUSIONS: Although differences exist in the medical procedures given to African Americans and whites with SCI, they are generally accounted for by cause of injury rather than the direct affects of race.     

Acquired brain injury, visual attention, and the useful field of view test: A pilot study

OBJECTIVE: To compare the findings of the Useful Field of View (UFOV) test with those of conventional neuropsychologic tests to determine the utility of the UFOV test as a measure of attention in a population with brain injury. DESIGN: Cohort study. SETTING: Freestanding rehabilitation hospital. PARTICIPANTS: Fifteen inpatients with severe brain injury. INTERVENTIONS: Not applicable. MAIN OUTCOME MEASURES: UFOV test, FIM\T instrument, length of stay (LOS), and standard neuropsychologic testing. RESULTS: The UFOV subtest UF2 correlated strongly with the other 2 subtests, UF1 and UF3. The UF2 subtest correlated most strongly with paper and pencil tests of visual attention. The UF2 predicted 52% of the FIM change and 60% of the LOS variance, second only to admission FIM score, which predicted 75% and 80% of FIM change and LOS variance, respectively. CONCLUSIONS: Among the patients in our study, the UFOV test can be used to determine the visual divided attention of patients with acquired brain injury. The results also showed that the UFOV test correlated with LOS and FIM change in patients with acquired brain injury recovering in a rehabilitation facility. Because the UFOV test is much more quickly administered and scored than other measures of attention and divided attention, these results suggest that the UFOV test may provide an easy means to measure a critical variable in the population with head injury.     

Wheelchair back-support options: functional outcomes for persons with recent spinal cord injury

OBJECTIVE: To evaluate the functional outcomes for people with a recent spinal cord injury (SCI) when using 3 different back-support options. DESIGN: Repeated-measures within-subject comparison. SETTING: Rehabilitation center. PARTICIPANTS: Twenty-seven manual wheelchair users with recent SCI. INTERVENTIONS: Three types of wheelchair back supports--a standard sling upholstery, a Jay J2 back, and a Pindot PaxBac--were used by each participant to evaluate performance of 4 functional tasks. MAIN OUTCOME MEASURES: Functional tasks included timed forward wheeling, ramp ascent, distance for forward vertical reach, and 1-stroke push. Product satisfaction was also rated. RESULTS: For most participants, best performance for each task varied with back support used. Significant differences in functional performance were found for the reaching activity (P=.01), with greater reach when the J2 was used. Satisfaction ratings also differed significantly (P=.017), with participants indicating greater satisfaction with the J2. Participants' choice of back support varied and did not consistently correspond with best performance. CONCLUSIONS: Except for reaching, performance of functional activities did not differ depending on back support used. Objective evaluation of reaching ability may assist in product decision making. Inclusion of a consistent battery of functional tasks could provide a standardized reference point for clients' subjective evaluations.