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81.
Benedita Sampaio-Maia Mónica Moreira-Rodrigues Paula Serr?o Manuel Pestana 《Nephrology, dialysis, transplantation》2006,21(2):314-323
BACKGROUND: A primary tubular sodium handling abnormality has been implicated in the edema formation of nephrotic syndrome. Dopamine synthesized by renal proximal tubules behaves as an endogenous natriuretic hormone by activating D(1)-like receptors as a paracrine/autocrine substance. METHODS: We examined the time courses of the urinary excretion of sodium, protein and dopamine in puromycin aminonucleoside (PAN)-treated and control rats. The rats were sacrificed during greatest sodium retention (day 7) as well as during negative sodium balance (day 14) for the evaluation of renal aromatic l-amino acid decarboxylase (AADC) activity, the enzyme responsible for the synthesis of renal dopamine. Also, the influence of volume expansion (VE) and the effects of the D(1)-like agonist fenoldopam (10 microg/kg bw/min) on natriuresis and on proximal tubular Na(+),K(+)-ATPase activity were examined on day 7. RESULTS: The daily urinary excretion of dopamine was decreased in PAN-treated rats, from day 5 and beyond. This was accompanied by a marked decrease in the renal AADC activity, on days 7 and 14. During VE, the fenoldopam-induced decrease in proximal tubular Na(+),K(+)-ATPase activity was more pronounced in PAN-treated rats than in controls. However, the urinary sodium excretion during fenoldopam infusion was markedly increased in control rats but was not altered in PAN-treated animals. CONCLUSION: PAN nephrosis is associated with a blunted renal dopaminergic system activity which may contribute to enhance the proximal tubular Na(+),K(+)-ATPase activity. However, the lack of renal dopamine appears not to be related with the overall renal sodium retention in a state of proteinuria. 相似文献
82.
83.
P Avalos-Peralta† A Herrera† JJ Ríos-Martín‡ AM Pérez-Bernal† D Moreno-Ramírez† F Camacho† 《Journal of the European Academy of Dermatology and Venereology》2006,20(1):79-83
We report the case of a patient with a 13-year history of pemphigus vulgaris (PV) treated with immunosuppressive agents, prednisone and mycophenolate mofetil who had developed lesions of Kaposi's sarcoma (KS) on a sole plaque of PV that had been previously treated with intralesional injections of steroids. The lesions were surgically removed and polymerase chain reaction (PCR) demonstrated human herpesvirus-8 (HHV-8) DNA. There were neither recurrences nor later dissemination of KS following gradual decrease of the immunosuppressive therapy. We suggest that the treatment with intralesional steroids may have influenced the local reactivation of a latent infection of the virus, determining the appearance of this localized KS. 相似文献
84.
85.
用小鼠败血症系统感染模型,以感染小鼠肾匀浆活菌计数为标准,以庆大霉素为对照药,探讨日剂量单次与分两次给药两种方案对新氨基糖苷类抗生素89—07体内抗留作用的影响。结果表明大肠杆菌90-020、绿脓假单胞菌3—374、金葡球菌90—506对抗生素89—07和庆大霉素均敏感(MIC范围0.0625~2mg/L);抗生素89—07和庆大霉素日剂量单次给药组(OD)3株实验菌的肾匀浆活菌计数值由给药前的4.94±0.09、4.60±0.27、4.86±0.101gcfu/ml分别降至1.94±0.19、1.84±0.25、1.88±0.251gcfu/ml(给药后7~8h).日剂量分两次给药组(BID)则分别降至2.95±0.48、2.87±0.37、3.44±0.271gcfu/ml。OD组对3株实验菌的最大杀菌作用Emax分别为3.02、2.97和2.851gcfu/ml,OD给药方案的体内杀菌作用明显优于BID方案。 相似文献
86.
Acute Immobilization Stress and Intraventricular Injection of CRF Suppress Naloxone-Induced LH Release in Ovariectomized Estrogen-Primed Rats 总被引:1,自引:0,他引:1
Tatsuo Akema Atsuhiko Chiba Reiji Shinozaki Morihiro Oshida Fukuko Kimura & Jun-ichi Toyoda 《Journal of neuroendocrinology》1996,8(8):647-652
The present study was undertaken to evaluate the role and possible interaction of the endogenous opioid peptide (EOP) and corticotropin-releasing factor (CRF) in the acute stress-induced suppression of gonadotropin secretion in ovariectomized estrogen-primed rats. An intravenous (i.v.) injection of naloxone (10 or 20 mg/kg), an EOP antagonist, significantly elevated serum luteinizing hormone (LH) levels within 10 min in non-stressed animals. The naloxone-induced LH release was completely eliminated when tested 30 min after the onset of acute immobilization. In a subsequent study, it was found that suppression of the naloxone-induced LH release occurred as early as 5 min after the stress onset, and was still evident 60 min after the end of a 30-min period of immobilization. The effect of naloxone was restored 3 h after liberation of the animal from the 30-min immobilization. An intraventricular (i.c.v.) injection of CRF (1 or 5 μg) also significantly suppressed, in a dose-related manner, the effect of a subsequent i.v. injection of naloxone. However, an i.c.v. injection of α -helical CRF(9-41) (25 or 50 μg), a CRF antagonist, prior to immobilization, could not interfere with the suppressive effect of stress on naloxone-induced LH release. These results suggest that both acute immobilization stress and CRF can inhibit the LH secretory activity without mediation by EOP neurons. However, the stress-related suppression may involve non-CRF mechanism(s). 相似文献
87.
We describe a simple direct extraction method for the gas-liquid chromatography determination of serum valproic acid. The working range for the assay is 2-180 mg/L and our within-run precision was 5.8 and 4.3% at the 40 and 90 mg/L concentrations respectively. Hemolyzed and lipemic sera as well as samples from patients with hyperbilirubinemia and from patients with decreased renal function were put through the assay and no interfering peaks were noted. Interference occurred when teflon-lined screw caps were used during the extraction step. The method was proven to be accurate by linear regression analysis of samples containing weighed-in amounts of valproic acid. The above assay was compared to an enzyme immunoassay technique (EMIT). The working range for the latter is 10-150 mg/L and the with-run precision was 10.8 and 5.9% and 90 mg/L concentration respectively. Samples were run by both the gas-liquid chromatograph and enzyme immunoassay methods and gave very similar results over the range 16-139 mg/L. 相似文献
88.
目的:研究多形性低度恶性腺癌(PLGA)的组织病理学和免疫组织化学特点,并与腺样囊性癌(ACC)进行比较。方法;采用组织病理学与免疫组织化学方法,对8例PLGA与10例ACC进行分析。结果;PLAC的特点是组织学上具有细胞形态的一致性与组织结构的多形性;免疫级化染色呈阳性或部分阳性反应,与ACC没有明显差异。结论:光镜下组织结构的多形性是鉴别PLGA与ACC的重要指征;免疫组化对二者的区分意义不大;均起源于闰管储备细胞是导致二者相似性的根本原因。 相似文献
89.
"立交桥"高职护理人才培养模式初探 总被引:3,自引:1,他引:2
为探讨中等卫生学校毕业就读高等护理专业学生(即“立交桥”高职)的培养模式,在坚持高等教育培养目标及职业特色的前提下,结合学生特点,进行课程设置、教学方法及临床实践等方面的改革,以发挥学生的优势,弥补学生的不足,培养适应现代护理发展需要的高质量护理人才。 相似文献
90.
目的:研究治疗前列腺增生的α1-受体拮抗剂SL-89.0519-08的合成路线。方法:以5-氯-2-甲氧基苯胺为原料,经环合,烃化,脱保护基和亲核取代等步骤,合成了目标化合物,同时对原料合成工艺进行了摸索。结果:合成的目标化合物经IR,^1HMNR,EI-MS和HRMS得以确证。结论:该合成工艺和路线适宜工业化生产。 相似文献