犬自体气管移植中骨形态发生蛋白复合不同载体的成软骨比较

目的：以三种可溶性物质作为重组人骨形态发生蛋白－2（rhBMP-2)的载体，寻求适合作为rhBMP-2在犬的自体气管移植段内诱发新生软骨的缓释载体。方法：32只犬随机等分4组，颈部切取5环气管段作为移植段。等量的rhBMP-2分别与葡聚糖、聚乙烯吡咯烷酮（PVP）和胶原复合，另设无载体作为对照，均注射入各移植段内。然后将移植段埋入自体的腹腔大网膜中。于术后4周处死动物取材，行大体及组织学观察。结果：移植段固有软骨环被部分地吸收。PVP和胶原组软件环间rhBMP-2复合物樾入区有大量新生软骨形成，且以胶原组更明显。结论：PVP和胶原均可协助rhBMP-2诱发气管移植段软骨再生，胶原更适合充当rhBMP-2的缓释载体。     

谈红斑狼疮及其临床检验

全身性(系统性)红斑狼疮．是一种原因不明的自身免疫性疾病，亦称胶原性疾病。可分为局限型和播散型两种。局限型．皮肤损害较明显，呈现淡红色，有毛孔扩张和粘连性鳞屑，边缘明显，多发生在面部，分布对称，呈蝴蝶型；播散型，红斑初起为鲜红色，对称分布，除面部外可发生在全身任何部位，尤其是四肢更为明显。     

胶原对小鼠烧伤创面愈合的影响

本实验将胶原用于小鼠烧伤创面局部,观察对创面愈合的影响,以探讨其应用价值。实验用18～22 g的小鼠20只,分为烧伤胶原治疗组和烧伤对照组。制作成7％Ⅱ度烧伤模型,伤后分别在创面涂用胶原和盐水(0.4ml/次,每日1次)。通过创面愈合率和光镜的观察,发观胶原对创面愈合有明显的促进作用。     

维生素E乳剂对肝脏星状细胞Ⅲ型前胶原和MMP1 mRNA表达的影响

肝纤维化的病理特征是细胞外基质（ＥＣＭ）在窦周间隙的大量沉积，特别是Ⅰ，Ⅲ型胶原更明显．肝纤维化动物模型研究表明，维生素Ｅ有抗肝纤维化作用［１］．我们观察维生素Ｅ对肝脏星状细胞的影响，以期从分子水平阐述维生素Ｅ抗纤维化作用机制，为临床用于慢性肝病的治疗提供更多的依据．１　材料和方法１．１　材料　肝脏星状细胞系（ＣＦＳＣ）由美国Ｇｒｅｅｎｗｅｌ教授建立；１０ｇ／Ｌ维生素Ｅ乳剂由重庆制药厂制备；ＲＰＭＩ１６４０培养基为Ｇｉｂｃｏ公司产品；Ⅲ型前胶原放免测定试剂盒为重庆肿瘤研究所产品；Ⅲ型前胶原探针、…     

低蛋白饮食对环孢素A肾病大鼠肾间质纤维化的作用

Objective To investigate whether low-protein diet has protective effect on the progression of renal interstitial fibrosis in rats with cyclosporine A (CsA)-induced nephropathy. Methods Eighteen male Sprague-Dawley rats were randomly divided into three groups (6 rats in each group). The rats in control group (C group) received common diet; in model group (M group) low-salt diet; in intervention group (Ⅰ group) low-salt and low-protein diet. After diet adaptation period of one week, the rats in C group received subcutaneous injection of olive oil 1 mg/kg daily for 5 weeks, while M group and Ⅰ group subcutaneous injection of CsA (diluted into 25 g/L with olive oil) 1 ml/kg for 5 weeks. All the rats were sacrificed at the end of the 5th week. The food-intake and body weight were measured daily. The creatinine clearance (Ccr) was examined before rats were sacrificed. The semi-quantitative pathological analysis on kidney sections was performed. The mRNA and protein expression of transforming growth factor-β1 (TGF-βI) and type Ⅰ collagen (Col Ⅰ) in kidney tissue was determined with real time PCR and immunohistochemical staining, respectively. Results The food-intake and body weight of rats in M and I groups were significantly lower than those in C group (P<0.05). Compared with C group, the Ccr levels in M and Ⅰ groups were significantly reduced [(0.65±0.15) ml/min, (0.40+0.13) ml/min vs (1.55±0.29) ml/min, P<0.05], the relative fibrosis areas of kidney interstitium in M and I groups were significantly increased (3.60%±0.46%, 3.26%±0.75% vs 0.44%±0.24%, P<0.05), the mRNA and protein expression of TGF-β1 in M and I group was significantly up-regulated (by 2.6 and 3.1 times in mRNA and by 1.5 and 1.6 times in protein, respectively, P<0.05), and the mRNA and protein expression of Col Ⅰ in M and I groups was also significantly up-regulated (by 3.0 and 3.5 times in mRNA and by 2.3 and 2.1 times in protein, respectively, P<0.05). There were no significant differences between M and I groups in every parameters above-mentioned except the rat body weight and Ccr. Both the body weight and Ccr in Ⅰ group were significantly lower than those in M group (P<0.05). Compared with C group, the urine osmotic pressure in M group and in I group were deceased (for M group, P>0.05; for I group, P<0.05). Compared with C group, the serum cholesterol levels in M and I groups were significantly increased (P<0.05), and the serum phosphorus level in I group was significantly decreased (P<0.05). The levels of serum albumin and serum calcium of all three groups had no statistical differences (P>0.05). Conclusion Low-protein diet has no renoprutective effects on the rat model of cyclosporin A nephropathy, on the contrary, may induce body weight loss.     

增生性玻璃体视网膜病变玻璃体中的Ⅲ型前胶原

目的:检测增生性玻璃体视网膜病变(PVR)患者血清及玻璃体中Ⅲ型前胶原(Procollagen Ⅲ,PC Ⅲ)的含量,探讨其在PVR发病中的作用。方法:用放射免疫测定法测定5例正常人玻璃体,20例正常人血清及29例孔源性视网膜脱离合并PVR患者玻璃体及血清中的PC Ⅲ。用Logistic回归模型分析PVR患者发病时间、手术史、增生膜及PVR分级与PC Ⅲ含量的相关性。结果:PVR患者血清和对照组血清PC Ⅲ含量分别为83.76±18.52和85.02±17.50μg/L(P>0.05)。正常对照玻璃体PC Ⅲ含量低于最小可测值(40 μg/L)。PVR患者组中有12例未检测到,其余17例玻璃体中PC Ⅲ含量明显升高,平均268.69±176.07μg/L(P<0.05)。玻璃体PC Ⅲ水平与病史长短和PVR级别相关,发病时间延长,其玻璃体PC Ⅲ含量大于40μg/L的概率是小于40 μg/L的5.2655倍;PVR级别增加,其玻璃体PC Ⅲ含量大于40 μg/L的概率是小于40 μg/L的2.7978倍。结论:多数PVR患者玻璃体内可测得PC Ⅲ含量增加,并与PVR分级及发病时间长短相关。PC Ⅲ合成活跃属眼内的局部反应。提示PC Ⅲ及Ⅲ型胶原在PVR发展中起一定的作用。眼科学报1998;14:76—79。     

血、腹水Ⅲ型前胶原肽联合检测在肝脏疾病中的临床价值

目的：探讨血、腹水Ⅲ型前胶原肽（PⅢP）联合检测在肝脏疾病中的临床价值。方法：采用放射免疫法同步测定50例对照组，25例肝硬化失代偿患者血、腹水中Ⅲ型前胶原肽含量变化。结果：肝硬化患者血、腹水中PⅢP含量均明显高于对照组，差异有统计学意义，腹水中PHIP含量明显高于血清PⅢP含量．P〈0．001；以120μg／L为临界值，计算其95％可信区间内其PⅢP敏感性、特异性、真实性均为腹水〉血。结论：肝炎后肝硬化患者无论血、腹水中PⅢP检测均可获得较高的敏感性和特异性。PⅢP含量测定对诊断肝纤维化有一定的价值，其血清含量变化在反映肝硬化、肝细胞坏死及肝功能损害程度方面具有一定的临床实用价值。     

雌二醇对人类成骨细胞HOSTE85增殖及I型胶原产生的影响

目的：雌激素替代疗法更年期骨质疏松的预防及治疗效果显著，但作用机理不很明确，本研究通过体外培养的人类成骨细胞ＨＯＳＴＥ８５，观察雌二醇对成骨细胞增殖及１型胶原产生的影响。方法：在体外培养的人类成骨细胞ＨＯＳＴＥ８５培养兴衰，加入雌二醇（０．０１～１０ｎｍｏｌ／Ｌ），分别培养２４，４８，７２及９６ｈ，于结束培养前６ｈ加入＾３Ｈ－胸腺嘧啶脱氧核苷检测细胞增殖，或于细胞开始培养时，加入＾３Ｈ－脯氨酸检测