肝纤维化过程中血清I型胶原与炎性介质关系的研究

用酶联免疫检测技术对慢性肝病患者血清Ｉ型胶原及ＴＮＦα进行了检测。结果表明，随着慢性肝病进行性发展，其血清含量逐渐增加。相关性分析表明二指标之间及与γ－球蛋白呈正相关。Ｉ型胶原与ＴＮＦα联合诊断可提高诊断符合率。以上结果提示，Ｉ型胶原和ＴＮＦα是反映肝纤维化程度的良好指标。ＴＮＦα对Ｉ型胶原的合成与分泌具有调节作用。胶原成分与细胞因子联合诊断肝硬化，可互相弥补其局限性，显示了联合怕优越性。     

慢性肝病患者血清透明质酸及Ⅲ型前胶原联合检测的临床意义

用放免法检测64例慢性肝病患者、20例正常人血清透明质酸（HA）及Ⅲ型前胶原（PCⅢ）。结果表明：慢性活动型肝炎、肝硬化病人血清HA及PCⅢ水平明显高于慢性迁延型肝炎及正常人，肝硬化病人血清HA及PCⅢ水平又显著高于慢性活动型肝炎病人，而慢性迁延型肝炎病人血清HA及PCⅢ水平与正常人相比无显著性差异。检测血清HA及PCⅢ对慢性肝病的诊断、预后判断及疗效观察均具重要临床意义，并且血清HA及KCⅢ联合检测优于HA或PCⅢ单项检测。     

各种肝病的肝纤维化标志改变的探讨

目的 :探讨各种肝病患者血清肝纤维化标志的改变 ,甘草酸二胺注射液对肝脏炎症、纤维化指标的作用。方法 :用放免法检测透明质酸 (HA)、层粘蛋白 (LN)、Ⅲ型前胶原多肽 (PCⅢ )。结果 :急性肝炎的三种肝纤指标均升高 (P <0 0 5 )。急黄肝高于急无黄肝 (P <0 0 5 )。慢性肝炎 1 1 2例 ,三项肝纤指标显著高于正常对照 (P <0 0 5 )。慢肝重度显著高于慢肝轻度 (P<0 0 0 1 )。肝硬化的三项肝纤指标又显著高于慢肝 (P <0 0 0 1 ) ,其它肝病的肝纤指标也升高。 2 5例慢肝用甘草酸二胺注射液治疗 2月 ,ALT、AST、GGT显著降低 (P <0 0 1 )。三项肝纤指标中HA降低较显著 (P =0 0 5 ) ,LN和PCⅢ虽有降低 ,但差异不明显 (P =0 4 5 9,P =0 1 92 )。结论 :各种肝病的三项肝纤维化指标均升高 ,以慢肝重度 ,肝硬化升高明显。甘草酸二胺注射液治疗有抗炎及改善肝纤维化的作用     

恶性淋巴瘤患者血清唾液酸、透明质酸、层粘蛋白、IV型胶原含量及临床意义

目的 :为探讨恶性淋巴瘤 (ML)患者血清唾液酸 (SA)、透明质酸 (HA)、粘层蛋白 (LN)、IV型胶原 (IVC)变化的临床意义。方法 :采用分光光度比色法及放射免疫分析法测定了 4 3例ML患者血清SA、HA、LN、IVC含量。结果 :ML患者血清SA、HA、LN、IVC明显高于正常人 (P <0 .0 5～0 .0 0 1) ,Ⅲ、Ⅳ期患者较Ⅰ、Ⅱ期明显增高 ;治疗达缓解后较化疗前明显下降 (P <0 .0 5～ 0 .0 1)。结论 :血清SA、HA、LN、IVC的变化可作为观察ML病情及监测治疗反应的一种手段     

Ⅱ型胶原与自身免疫性内耳病

1979年，McCabe发现某些感音神经性聋的临床表现及实验室检查特征提示其为自身免疫性疾病。其特征为：数周或数月内发展的渐进性聋(而非数小时、数天或数年)；感音神经性聋通常为双侧或非对称性；耳蜗常受累；常伴有面瘫和前庭功能不良；可有鼓膜、中耳和乳突组织破坏；对大剂量类固醇激素和环磷酰胺疗效好。McCabe将此类疾病定义为：自身免疫性内耳病(autoimmune inner ear disease，AIED)。现认为AIED可理解为两重范畴：一为狭义，如McCabe所描述，即AIED为一独立的疾病实体；一为广义，即与自身免疫相关的一组内耳疾病，包括梅尼埃病、耳硬化症和狭义范畴的AIED等。本文所指为其广义范畴。     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

儿童胸肺区恶性小细胞瘤1例报告

患儿男，14岁，因反复右胸痛2个月，近1周阵发性干咳入院。伴有精神差、盗汗、消瘦，偶有发热。在当地医院胸片示“右侧胸膜炎并大量胸腔积液”。曾胸穿抽出暗红血液约5ml，抽出即凝。体检：T39℃，P145次／分，R30次／分，BP14．6／93kpa，慢性病容，气管左偏，右胸隆起，右肺呼吸运动、触觉语颤明显减弱，     

肾小球基膜Ⅳ型胶原研究进展

由于慢性肾脏病最终发展为肾功能衰竭，病理形态上表现为肾小球硬化，即基膜增厚和系膜基质的增加，那么作为ＧＢＭ的主要构成成分的Ⅳ型胶原其中起什么作用呢？它随病病变化又有什么规律？值得探讨。通过对Ⅳ型胶原的深入研究，对Ｇｏｏｄｐｓｔｕｒｅ综合征和Ａｌｐｏｒｔ综合症等发病机理有了进一步的认识。     

甲状腺功能亢进患者骨代谢指标和甲状腺激素水平变化及其临床意义

为探讨甲状腺功能亢进(甲亢)患者骨代谢指标与甲状腺激素水平的变化, 本文对70例甲亢患者及60名健康对照者采用放射免疫分析法(RIA)测定了Ⅰ型胶原羧基末端肽(ICTP), 全自动微粒子化学发光分析法测定了骨碱性磷酸酶(BAP)及甲状腺激素(T3、T4、FT3、FT4)水平, 同时用二维骨密度仪测定骨密度(BMD).结果表明: 甲亢患者血清BAP、ICTP与甲状腺激素水平均明显高于健康对照组(P均小于0.01), 骨密度(BMD)则显著低于健康对照组(P＜0.01).相关分析显示, 甲亢患者血清BAP、ICTP水平与BMD呈明显负相关(r分别为-0.298和-0.276, P＜0.05), 与T3呈正相关(r分别为0.452和0.531,P＜0.01), 与T4亦呈正相关(r分别为0.419和0.398, P＜0.01).提示甲亢患者骨转换增强, 并以骨吸收增加更为显著, 与甲亢时甲状腺激素分泌过多有关. 定量检测血清BAP、ICTP水平对于甲亢代谢性骨病的诊断、病情研究均有重要的临床价值.