去细胞喉支架的制备及喉肌细胞再生的可行性研究

Objective To prepare a deeelhilarized whole laryngeal scaffold by utilizing a perfusion-decellularized technique, reseed cells on it, and construct recellularized laryngeal muscles. Methods Perfusion decelluarized larynxes were obtained by common carotid arterious perfusion with detergents. Then they were performed by macroscopic view, histological examination, scanning electron microscopy (SEM) and cartilage viability. Decellularized laryngeal scaffold were then reseeded with inducted mesenchymal stem cells (MSCs). Composites were transferred into greater omentums of rabbits after one day' s adherence and harvested after eight weeks. Macroscopic view, histological examination and immunohistochemistry were performed. Results Perfusion larynxes became transparent after two hours. Histology and SEM indicated that perfnsion method shewed better deculluarized effect. More ventages and collagen fibers but no intact cell or anclei were retained in the decellularized martrix. Porosity measured by Image pro plus 6. 0 was 80. 4% ± 3.2% (x ± s). Chondrocyte vitality assay indicated chondrocyte vitality rate in the perfusion group was 86. 9% ± 1.5% . After eight weeks, vascularization formed and integrated cartilage frameworks still remained. Histological examination could clearly show the presence of muscle bundles and vessels. Immunohistoehemical examination indicated that sarcomeric-α actin expressed positively in corresponding areas. Conclusions It is feasible to reseed MSCs into the decellularized laryngeal muscle matrix for constructing tissue-engineered laryngeal muscles. This in vivo maturation into the omentum could be the first step before in situ implantation of the construct.     

炎症性肠病生物治疗评价

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成都仪器厂生物信号采集与处理系统等产品简介

Rm6240／Rm6280／Rm62160（四、八、十六道）生理记录仪暨生物信号采集与处理系统 ★Rm6240B／C、Rm6280C、Rm62160C生理记录仪暨生物信号采集与处理系统是成都仪器厂研制的新一代医学实验设备，产品获得了医疗仪器准产注册证、计量器具生产许可证等有关证照，符合医疗仪器及药品监督管理的有关法规。隔离型产品既可用于人体实验也可用于动物实验。     

心肌梗死的干细胞移植治疗新进展

心肌梗死(心梗)是目前严重危害人类健康的疾病之一,由于成体心肌细胞缺乏足够再生能力,因而梗死后将导致心肌细胞不可逆的丢失,并被纤维瘢痕组织取代,最终导致心力衰竭。心衰是心梗致死的主要原因之一。如何促进缺血坏死区心肌再生,防止心室重构,已成为防止心梗后心衰发生及降低远期病死率的关键。近年来随着分子生物学和细胞生物工程技术的发展以及对干细胞研究的深入,通过细胞移植技术,试图将干细胞移入受损心肌组织中分化为心肌细胞,替代坏死心肌改善心功能。这为心梗后心衰提供了一种全新的治疗策略。本文就近年来国内外在干细胞移植治…     

自体干细胞移植在烧创伤研究领域的应用

成体干细胞的可塑性(plasticity)是目前临床治疗性应用的基础，但一直备受争议。特别是2002年Nature杂志发表了Terada和Ying等将标记有绿色荧光蛋白(GFP)的骨髓间充质干细胞(MSC)与胚胎干细胞(ES)进行共培养，观察到形成GFP ES样细胞后，这种争论与质疑便进一步受到人们的关注，并引发了相关论战。但在争论的背后，临床的     

日采用小鼠胚胎干细胞培育出人造肝脏

据医业网11月8日报道，日研究人员用小鼠胚胎干细胞培育出生物人造肝脏，这种人造肝脏植入肝功能衰竭的小鼠体内后，可使小鼠肝脏功能恢复正常。     

造血干/祖细胞动员临床研究新进展

本文综述了干细胞动员的发展史,动员外周血干/祖细胞的特点与外周血干细胞移植的优势,介绍了外周血干细胞的动员方法与影响异基因干细胞的动员的因素。     

医学突破：睾丸细胞可能制成心脏

国际间对干细胞的研究又有了重大的突破!美国一家生物科技公司,近日在西班牙一场会议中发表最新研究成果,声称已从人类睾丸中取出未成熟的细胞,改造为干细胞。这项成果若经过证实,将在人体胚胎之外,提供另一个干细胞的来源。美国加州的研究人员表示,他们已从一群年龄26至50岁的男士睾丸中取出未成熟细胞,再将细胞重新排列为干细胞,并利用这些干细胞培植出神经心脏及骨骼细胞等。