乙酰葛根素对大鼠局灶性脑缺血再灌注损伤后一氧化氮和内皮素生成的影响

山东大学学报(医学版),2005,43(12):1173-1177.目的:探讨乙酰葛根素在脑缺血再灌注损伤后对一氧化氮(NO)和内皮素(ET)生成的影响。方法:采用大脑中动脉内栓线阻断法造成大鼠局灶性脑缺血再灌注损伤(假手术组除外)后,随机将实验大鼠分为6组,连续灌胃给药10d:损伤组[生理盐水5mL/(kg·d)];对照组[葛根素50mg/(kg·d)];乙酰葛根素高、中、低剂量组[乙酰葛根素250、50、10mg/(kg·d)];假手术组[生理盐水5mL/(kg·d)]。采用生化法测定脑缺血1h再灌注24h及48h大鼠脑组织匀浆和血清中NO水平,用放射免疫法测定血浆中ET水平,并进行脑组织病理学检…     

颈前筋膜注药治疗神经根型颈椎病的临床观察

目的:探讨神经根型颈椎病的临床特点及治疗,方法,本组病例采用颈前筋膜注射药物。结果:治疗后随访1个月到半年,本组51例,临床治愈30例,显效16例,有效5例,总有效率100%,结论:颈前筋膜注药治疗神经根型颈椎病,疗效满意,可作为传统颈椎牵引,按摩疗法的补充。     

川芎嗪联合降纤维酶治疗脑梗死的疗效观察

我科于2003年1月-2003年12月的40例急性脑梗死(ACI)患者分别给予川芎嗪和降纤酶及常规治疗,取得较好疗效,现报道如下。一般资料:全部患者共80例,均为住院病人,均有局灶性神经功能缺损,符合1995年全国第四届脑血管病学术会     

杨绍伊先生《考次汤液经序》(续)

广论之惑已明,再辨叔和撰次.<甲乙经·序>又云:"近世太医令王叔和,撰次仲景遗论甚精."①案今本仲景书卷端,即题云"王叔和撰次".以士安言解之:所谓"撰次"者,即撰集仲景遗论,以之次入仲景书中是也.若然,则今本仲景书为任圣之<汤液经>、张仲景之<广论>、王叔和之<仲景遗论撰>三种集合而成.求之叔和撰次书,见<辨太阳病>首篇.     

益气活血法加穴注治疗气虚血瘀型胸痹42例

笔者自2000年来用自拟益气活血通痹汤口服加生脉注射液，丹参注射液交替穴位注射治疗气虚血瘀型胸痹，取得满意的的临床疗效。     

肾上腺素在局麻中的应用观察

目的探讨局麻药中加入适量肾上腺素对手术患者出血量、麻醉时间、及心率、血压、心电图的影响。方法将2005年7月至2009年7月在手术室进行手术的患者200例，随机分为试验组和对照组各100例；对照组采用1％的利多卡因15ml进行局麻，观察组用1％的利多卡因15神加。肾上腺素5μg／Hd进行局麻；比较局麻部位组织及局麻前后两组的心率、血压和心电图等的变化。结果观察组患者的术中出血量明显减少，平均出血量仅为对照组的1／5，观察组麻醉止痛时间延长，为对照组的2—3倍；两组患者的心率、收缩压、舒张压、心电图波形及节律局麻前后无明显变化（P〉0．05）。结论局麻药中加入适量肾上腺素能够减少出血，延长麻醉时间，对患者的心率、血压及心电图等无明显影响，在局麻手术患者中值得推广。     

硬膜外腔麻醉下注药加手法治疗腰椎间盘突出症391例

我院自 1993年以来用硬膜外麻醉下注药加手法治疗腰椎间盘突出症 ,取得了满意疗效。现报告如下。1　一般资料本组 391例 ,女 187例 ,男 2 0 3例。突出部位 :L4～L52 12例 ,L5～S192例 ,L3 ～L418例 ,L4～L5合并L5～S16 9例。上述病例分别进行腰椎X线摄片、CT断层扫描或MRI影像检查 ,临床上均有腰痛、下肢放射痛、下肢感觉异常等腰椎间盘突出症的主要体征。2　治疗方法2 .1　麻醉注药均采用硬膜外腔麻醉。取L2 ～L3 间隙 ,用 1.5 %利多卡因针 15ml作硬膜外腔麻醉。在麻醉结束拔管前 ,取地塞米松针 10mg用生理盐水稀释到 5ml,从硬膜外…     

丹红注射液治疗椎-基底动脉缺血性眩晕96例

椎-基底动脉缺血性眩晕是一种临床常见病。我院从2006年5月-2007年5月收治了182例椎-基底动脉缺血性眩晕患者，96例应用丹红注射液治疗，并与盐酸培他啶注射液治疗的86例相对比，现将结果报道如下。     

Poststroke DNA immunization against neurite growth inhibitors is beneficial to the recovery from local cerebral ischemia in rats

BACKGROUND： Inhibitory signals, i.e. neurite growth inhibitors （NGIs）, presenting on central nervous system （CNS） myelin have been shown to play a crucial role in inhibiting lesioned axonal sprouting and leading to less functional recovery. Vaccines targeting NGIs may provide multifactorial protection against brain insults by overcoming the inhibitory effects of these NGIs and boosting the body＇s immune repair mechanisms. OBJECTIVE： To evaluate the effect of poststroke DNA immunization against NGIs on the rehabilitation for sensorimotor function of rat models of local cerebral ischemia. DESIGN： Completely randomized grouping design, and controlled experiment. SETTING： Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore. MATERIALS： Sixty adult male Sprague-Dawley rats ranging in age from 45 to 120 days and in weight from 180 to 250 grams were provided by Animal Center of Department of Anatomy, Faculty of Medicine, National University of Singapore. pcDNA3.1（＋）-neurite growth inhibitors （pcDNA-NGIs） a gift was provided by Dr. Xiao from Department of Clinical Research, Singapore General Hospital, Singapore. METHODS： The experiment was carried out at Brain Injury Research Laboratory, Department of Neurosurgery, National Neuroscience Institute, Singapore from August 2003 to April 2005. （1）The involved rats were randomized into 3 groups： pcDNA-NGIs group （group A）, pcDNA3.1 （＋） group （group B） and model group （group C）, with 20 rats in each group. Left focal cerebral ischemia （FCI） was permanently induced through middle cerebral artery occlusion （MCAO） with the assistance of an operating microscope. Successful MCAO was determined by a 20% decrease to baseline in the ipsilateral cerebral blood flow. 100 μg of pcDNA-NGIs eluted in phosphate-buffered saline （PBS） was intramuscularly injected into the tibial muscle once a week after MCAO for 6 weeks in group A. As control, pcDNA3.1 （＋） was also administrated in the same way in group B and nothing was administrated in group C. （2） The modified neurological severity score （mNSS）, a composite of motor, sensory, reflex and balance tests, was used to test the sensorimotor deficit. The mNSS was graded on a scale of 0 - 18, i.e. normal score was 0, maximal deficit score was 18, and 1 point was warded for the inability to perform the tasks or the lack of a tested reflex. （3） The newly generated axons of corticorubral projection were traced by stereotaxic guided injection of 100 g/L biotinylated dextran amine. Rats were sacrificed two weeks after tracing, and cryostat coronal sections of midbrains （30μm） were reacted to BDA according to the manufacturer＇s instruction by the free-floating method. Images were captured on a DM RXA2 LEICA Microscope with a Spot Digital Camera system （Germany）, and the numbers of labeled axons on the denervated side in four standard coronal sections including the red nucleus were manually quantified. MAIN OUTCOME MEASURES： （1） The number of newly generated axons of corticorubral projection. （2）The improvement in sensorimotor deficit. RESULTS： All the involved 60 rats entered the stage of final analysis. （1） The number of newly generated axons of corticorubral projection of rats： Only ipsilateral axons of CRP were noted with little evidence of fibers crossing to the contralateral red nucleus in rats of groups B and C. More BDA-positive fibers crossing the midline and terminating in the contralateral red nucleus in appropriate target areas mirroring the non-differentiated red nucleus were found in rats of group A. Quantitative analysis showed that BDA-labeled axons in the denervated side of rats in group A were more than those in group B （P 〈 0.05）. （2） Improvement in sensorimotor deficit of rats： At 2 weeks after immunization, significant improvement in sensorimotor deficit was found in rats of group A. There were significant differences of improvement in sensorimotor deficit of rats between group A and group B or group C at 12 and 14 weeks after immunization （P 〈 0.05）. CONCLUSION： （1） Poststroke DNA immunization against NGIs leads to increased sensorimotor recovery following FCI and compensatory newly growth of axons from corticorubral projection.