高温、亚低温对大鼠脑缺血再灌注损伤的作用及其机制研究

目的：研究轻度高温、亚低温对大鼠脑缺血再灌注损伤组织兴奋性氨基酸（EAA）与氧自由基的相互关系及病理损伤程度的影响。方法：60只Wistar大鼠按不同脑温条件随机分为生化组（n=28）和病理组（n=32)，采用改良Nagasawa局灶脑缺血再灌注模型，观察脑缺血再灌注损伤组织谷氨酸（Glu），超氧化物歧化酶（SOD）、丙二醛（MDA）的变化及光镜，电镜下的病理变化。结果：轻度高温明显加重常温脑缺血再灌注损伤组织Glu、MDA的升高（P＜0．01）及SOD的下降（P＜0．05），加重常温脑缺血再灌注组织病理损伤程度，亚低温的作用则相反，结论：轻度高温可能通过同时促进EAA合成，释放和氧自由基生成系统活化，造成大鼠脑缺血再灌注损组织损伤加重；亚低温可能通过同时抑制EAA合成，释放和氧自由基生成系统活化，减轻大鼠脑缺血再灌注组织损伤程度，对大鼠脑缺血再灌注损伤组织起保护作用。     

青霉素皮试速发性过敏性休克抢救前后1例报告

1典型病例 患儿，女，12岁，发热，咽痛伴全身乏力3天入院治疗。既往无药物过敏史。查体：T38．9℃．P90次／分，BP100／60mmHg，发育正常，营养不良，神清，皮肤粘膜无黄染，浅表淋巴结无肿大，无鼻翼扇动，口唇无紫绀，咽部充血，双侧扁桃体1度肿大。颈软，心肺无异常，腹平软，腹部皮下脂肪明显减少，无压痛反跳痛，肝脾肋下未及，神经系统未见异常。     

痔瘘术后感染的原因分析和防治

痔瘘手术因肛门周围是大汗腺比较集中的地方，皮下脂肪丰富，还经常要受到粪便、尿液等污染，而且该处透气性较差，有利于各种细菌的生长繁殖，因此，在术前准备时，手术操作中和术后护理时都必须非常重视，防止感染的发生，相反如认为肛门部手术是个有菌手术而不加注意，则极易造成局部或严重的全身感染。     

牙保护托在全身麻醉下咽喉手术中的应用

随着医疗水平的提高,全身麻醉技术已日臻完善,加之人们对全身麻醉技术的认识逐渐深入,已逐渐摒弃了过去对全身麻醉的错误认识。尤其在儿童手术时更是出于担心局部麻醉可以增加患儿的恐惧心理,易造成长期的心理疾患,因此,儿童手术时全身麻醉比例有逐年增加的趋势。全身麻醉下耳鼻喉科咽喉手术时经常需用Davic开口器、支撑喉镜等,若准备不充分,可能造成一些副损伤,如牙齿松动、破碎或脱落等。因此我们设计牙保护托如图1,可有效避免上述副损伤的发生。国内专著有相似报道[1]。图1我们回顾性分析了北京航天总医院及沈阳市妇女儿童医院自1999年7…     

肠套叠患儿的临床护理观察

一段肠管套入相邻的肠内称为肠套叠，是婴幼儿急腹症之一，也是最常见的急性肠梗阻疾患，4～10个月的小儿发病率最高。据统计，山东省肠套叠发病率在急腹症中占第3位。我科2001-2004年收治28例肠套叠患儿，均为10个月内的婴幼儿。经临床护理观察，采用非手术治疗的17例中14例空气灌肠整腹，3例钡剂灌肠整腹；手术复位的11例中8例行肠套叠手术松解，3例行肠管坏死切除。住院时间最长15d，最短1d。现将护理观察介绍如下。     

夏练得法益寿祛病

夏季高温闷热，人体消耗特别大，各器官的衰老比其他季节更为明显。如果在这一时间能够坚持锻炼，其抗衰健体效果将更加显著。     

脊柱矫形术中唤醒试验的麻醉体会

<正>脊髓损伤是脊柱外科矫形手术中最严重的并发症,迄今为止仍未能完全避免,当患者手术结束完全清醒后发现脊髓损伤,虽然立即去除器械,采取各种措施,但脊髓功能仍不能完全恢复。     

超短波治疗输卵管不通的体会

输卵管不通其主要原因是由于各种感染因素所引起的输卵管炎症，如全身感染或人工流产，药物流产等其它原因引起的逆性型感染，炎症长期不愈，组织水肿及纤维组织大量增生，使输卵管不通，形成输卵管性不孕。如果堵塞不全，即输卵管通而不畅，易发生宫外孕。临床上经超短波治疗效果明显，将治疗体会介绍如下：     

Changes in T .lymphocyte subsets after severe traumatic brain inJury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.