肺腺癌和癌旁组织蛋白质表达谱差异的研究

通过比较肺癌患者癌组织和癌旁组织的二维凝胶电泳图谱(two-dimensional electrophoresis,2DE),以寻找肺癌相关蛋白、肺癌患者癌组织和癌旁组织,采用固相pH梯度(immobilized pH gradient,IPG)2DE分离总蛋白质,凝胶经银染显后,ImageMaster 2D图像分析软件进行比较分析、识别差异表达的蛋白质并进行质谱鉴定获取肺癌相关蛋白信息。结果分析得到肺癌组织蛋白点709个,癌旁组织蛋白点722个,选择表达量差异达3倍以上的40个,进行胶内酶解,基质辅助电离解析飞行时间质谱(MALDI TOF MS)获得肽质量指纹图谱(PMF),搜寻蛋白质数据库,得到7个肺癌标志物的候选蛋白,但这些差异蛋白点在肺癌发生发展中的作用需要进一步研究。     

带蒂颊肌瓣鼻底转移术与硅胶鼻底植入术治疗萎缩性鼻炎的疗效比较

目的：比较带蒂颊肌瓣鼻底转移术与硅胶鼻底植入术治疗萎缩性鼻炎的疗效。方法：将80例萎缩性鼻炎患者分为2组,43例行带蒂颊肌瓣鼻底转移术（A组）,37例行硅胶鼻底植入术（B组）。结果：手术治疗后总有效率A组高于B组（P＜0.05）,术后并发症A组少于B组（P＜0.05）。结论：带蒂颊肌瓣鼻底转移术治疗萎缩性鼻炎具有更好的疗效。     

Reciprocal Interaction Between Intestinal Microbiota and Mucosal Lymphocyte in Cynomolgus Monkeys After Alemtuzumab Treatment

It has been known that the gut microbiota plays a central role in shaping normal mucosal immunity, however, little information is available whether the variability of mucosal lymphocytes impacts the commensal flora. Here, we applied a cynomolgus monkey model to characterize the structure and composition of the gut microbiota in response to lymphocyte depletion and to determine their potential association. Molecular profiling of 16S rDNA showed that the intestinal microbiota composition was perturbed after the depletion of mucosal lymphocytes and were recovered following the repopulation. Some specific bacteria from the orders Lactobacillales, Enterobacteriales and Clostridiales, and the genus Prevotella and Faecalibacterium, were primarily responsible for the variations of the gut microbiota after lymphocyte depletion. Interestingly, the species richness of the ileal mucosal microbiota was associated the proportions of TCRαβ⁺ or TCRγδ⁺ T cells (p < 0.01). We demonstrate for the first time the feature of intestinal microbiota composition after lymphocyte depletion and provide novel evidence that the perturbation of gut microbiota is associated with lymphocyte depletion. It may contribute to understand the relationship between gut commensal microbiota and mucosal immune system. Study results provide insight into biological activity of alemtuzumab in intestinal barrier in organ transplantation.     

乙酸纤维膜电泳法快速分离磷酸肌酸激酶同功酶

采用乙酸纤维膜(涤沦基型)电泳法对CPK同功酶进行分离。所用缓冲液为Tris-巴比妥—巴比妥钠缓冲液,pH8.8,离子强度0.029M。电泳电压300V,时间10min。该方法不仅操作简便,分离效果好,重复性强,更重要的是省时价廉,适合临床急诊工作的需要。     

聚丙烯酰胺水凝胶注射隆乳材料清除的临床研究

目的 探讨分析采用不同方式清除聚丙烯酰胺水凝胶的适应症和手术方法.方法 对212例患者针对不同病情及要求,采取经乳晕切口单纯清除注射材料、清除材料同时行假体植入、清除材料同时行乳房提升、经乳腔镜清除材料等不同手术方式进行处理.结果 212例患者中单纯经乳晕切口清除139例,乳腔镜清除15例,同期植入假体46例,行乳房提升12例.大部分患者效果满意.结论 注射聚丙烯酰胺水凝胶患者要求清除者,应根据患者的具体注射情况及不同要求,在充分进行术前评估的基础上,采用合理方法进行处理,尽可能减少材料残留,达到满意效果,恢复乳房美观形态.     

Elucidating the Effects of Reaction Time on the Physicochemical Characterization of Valorized Synthesized Alumina

Aluminum waste-can management in Malaysia has recently become a serious environmental and public health issue, particularly in metropolitan areas. This has prompted the need to valorize these waste-cans into value-added products using the most economical and environmentally friendly techniques. In this study, the sol–gel technique was used to synthesize high-quality alumina from the aluminum waste-cans collected. From this method, the observed peaks of the synthesized alumina were identified as diaspore (α-AlO(OH)), boehmite (γ-AlO(OH)), aluminum oxide, or gamma-alumina (γ-Al₂O₃) crystalline structure and corundum. The morphological configuration, microstructure, and functional group properties of the synthesized alumina were evaluated. All the synthesized alumina exhibited a non-spherical shape and appeared to have hexagonal-like shape particles. Moreover, the XRD patterns of the synthesized alumina AL-6-30 and AL-12-30 exhibited a small angle (1–10°) with no XRD peak, which indicated a mesoporous pore structure with no long-range order. The overall results of γ-alumina synthesized from the aluminum waste-cans showed an optimal condition in producing a highly structured γ-alumina with excellent surface-area characteristics. The synthesized alumina exhibited stronger and highly crystalline functional characteristics almost comparable with the commercially available brands on the market.     

Testosterone gel for the treatment of male hypogonadism

Background: Testosterone supplementation is a standard treatment for young men with hypogonadism and has also been advocated as a treatment for symptomatic older men with low testosterone levels. Several modes of testosterone replacement are available including capsules, patches, injections, buccal tablets and gels. Objective: To review the literature concerning therapeutic efficacy and potential limitations of testosterone gels. Methods: The FDA medical reviews and the computerised database PubMed were searched for clinical trials studying currently approved testosterone gels. Results/conclusions: Testosterone gel has proved to be a valuable contribution to the existing modes of testosterone treatment, combining therapeutic efficacy, convenience and safety.     

手机二维码在医疗设备信息管理中的应用探索

杭州市红十字会医院将手机二维码技术应用于医院医疗设备的验收、核查、巡检、维修、防伪、技术培训以及设备库房管理、个人名片识别等领域,取得了很好的实践效果;分析了手机二维码在医疗设备管理领域的应用前景和面临的问题。     

上海市宝山区2015—2018年腹泻病人沙门菌血清分型和分子分型

目的 揭示上海市宝山区腹泻病人分离沙门菌的血清型和分子分型分布特征。方法 对2015—2018年分离的118株沙门菌进行血清分型和脉冲场凝胶电泳（pulsed-field gel electrophoresis,PFGE）分型,通过构建聚类树揭示不同血清型菌株的分子分型特征。结果 118株沙门菌分为25种血清型,前三位为肠炎沙门菌（45株,38.1%）、鼠伤寒沙门菌（26株,22.0%）、伦敦沙门菌（11株,9.3%）。其余22种血清型分别只包含1~3株菌。118株沙门菌通过PFGE分为71个PFGE带型,每个带型分别包含1~19株菌。45株肠炎沙门菌分为8个PFGE带型,相似度为75.0%~100.0%,在聚类树上形成一个簇;26株鼠伤寒沙门菌分为21个PFGE带型,相似度为48.0%~100.0%,在聚类树上形成两个簇;11株伦敦沙门菌分为8个PFGE带型,相似度为80.0%~100.0%,在聚类树上形成一个簇。结论 肠炎沙门菌和鼠伤寒沙门菌是上海市宝山区腹泻病人沙门菌优势血清型,但近年伦敦沙门菌有增多的趋势。肠炎沙门菌在本地区传播流行过程中的遗传变异相对比较保守,鼠伤寒沙门菌基因型多态性大,而且存在两个克隆。     

聚丙烯酰胺水凝胶毒副作用的实验研究

目的 通过动物实验探讨聚丙烯醇胺水凝胶应用于人体的安全性。方法 应用琼脂覆盖法和MTT法评价其对细胞的毒性。将聚丙烯酰胺水凝胶注入SD鼠皮下，定期取材，行大体及组织学观察。结果 两种方法测得的细胞毒性均在2级以上；注射局部轻度组织学反应，仅形成菲薄的包膜，其形态和位置不稳定，不能完全回抽，质地逐渐变硬；对鼠肾脏有一定的毒性反应。结论 聚丙烯酰胺水凝胶对细胞及肾脏有不同程度的毒副作用，作为软组织填充剂应用于临床应取审慎的态度。