Richner-Hanhart's Syndrome: New Ultrastructural Observations on Skin Lesions of Two Cases

New ultrastructural observations are described in skin lesions of two brothers with Richner-Hanhart's syndrome (RHS). Physical examination of the two patients showed painful skin lesions of palms and soles combined with denderitic corneal ulceration and mental retardation. The diagnosis of RHS was confirmed biochemically with high tyrosine levels in both blood and urine. Examination by transmission electron microscopy revealed several abnormal ultrastructural changes in the epidermal cells. The horny cells contained heterogeneously, electron-dense cytoplasm with many lipid droplets. The granular cell cytoplasm contained abundant tonofibrils and keratohyaline granules. The spinous cell cytoplasm was vacuolated due to the presence of minute tyrosine crystals, which are known to have a lytic effect. The surrounding keratinocytes contained multilobed nuclei. The basal epidermal cells appeared normal except for Merkel cells, which were severely damaged by vacuolatio, also due to the presence of tyrosine crystals. This study showed that high tyrosine levels can induce several ultrastructural pathological changes in the epidermal cells, including the skin chemoreceptor Merkel cells.     

活动力不足精子尾部微管超微结构研究

以２２例活动力不足精子病人的精液为标本，对其精子进行形态学研究，以了解精子尾部的形态变化。电镜观察发现：１例精子尾部形态变化较一致。轴丝排列为９＋０微管形式；另１例精子尾部形态变化呈多样性，或部分周围微管和部分外围致密纤维以及中央微管消失，或所有微管和外周致密纤维排列混乱。结果显示部分活动力不足精子病人精子尾部的微管异常和Ｋａｒｔａｇｅｎｅｒ‘ｓ综合征特征相似，这种异常可能影响精子的运动能力，而导     

Andrology: Deleterious effects of khat addiction on semen parameters and sperm ultrastructure

The semen parameters and sperm ultrastructural morphology havebeen described in semen samples from two groups of Yemeni subjects.The first ‘exposed’ group comprised 65 khat addicts,while the second control group included 50 non-khat addict subjects.The mean age was 39.94 ± 13.85 and 35.72 ± 11.35years in the exposed and control groups respectively, withouta significant difference. The mean duration of khat addictionamong the addicts was 25.34 ± 12.96 years (range 6.00–48.00).Statistically significant differences were detected betweenthe semen parameters of the two groups. Such parameters, includingsemen volume, sperm count, sperm motility, motility index andpercentage of normal spermatozoa, were lower among addicts.Significant negative correlation was also found between theduration of khat consumption and all semen parameters (r rangedfrom –0.30 to –0.74). At the transmission electronmicroscopy level, a counting system was incorporated to comparethe numbers of normal spermatozoa with deformed and dead spermatozoain ultrathin plastic sections. The total mean percentage ofdeformed spermatozoa was {small tilde}65%. Different patternsof sperm deformation were demonstrated, and included both thehead and flagella in complete spermatozoa, aflagellate heads,headless flagella and multiple heads and flagella. Deformedheads showed aberrated nuclei with immature nuclear chromatinand polymorphic intranuclear inclusions; these were associatedwith acrosomal defects. The deformed flagella demonstrated numericaberrations of the axonemal 9 + 2 configuration and structuraldefects of their associated elements. Persistent cytoplasmicdroplets were observed frequently. This study has shown forthe first time the deleterious effects of khat addiction onsemen parameters in general and sperm morphology in particularof all addicts, especially those who have consumed khat forlonger periods of time.     

黄芪多糖对小鼠脾脏急性放射损伤修复作用的实验研究

用组织学和组织化学方法动态地观察了黄芪乡糖(APS)对受5G⁶⁰coY线照射后小鼠脾脏及外周血淋巴细胞的作用。结果表明:APS可以促使损伤的脾脏组织结构明显恢复,以用药后第20天、第28天用药组与对照组变化差异有非常显着性(P<0.01和P<0.01),可以改善脾脏内淋巴细胞的超微结构,使线粒体增多,形态正常,提高外周血淋巴细胞内琥珀酸脱氢酶(SDH)的活性(P<0.01).     

黄连及其复方杀伤鼻咽癌细胞形态学观察

对黄连及其复方杀伤鼻咽癌细胞的形态改变进行了观察。发现Ⅰ、Ⅱ、Ⅲ号药作用６小时，各组ＨＮＥ１细胞无明显差别，均表现为轻微的细胞形态变化，细胞解离，细胞边缘毛糙。电镜下见粗面内质网扩张，线粒体轻度肿胀，核周间隙扩大，核染色质凝集。     

Lymphoglandular complexes of the colon: structure and distribution

The structure and distribution of lymphoglandular complexes of the colon are described. The cellular composition of these complexes, as detailed by immunohistochemistry and electron microscopy, suggests that they are sites of antigen processing. Variations in structure and in the number of complexes that are found in certain colonic diseases are documented.     

Brown Bowel Syndrome

The brown bowel syndrome (BBS) is a rare disease characterized by malabsorption and accumulation of lipofuscin in the smooth muscle cells of the muscularis externa of the small intestine. Recently, we incidentally observed a case of BBS in a colon operated on because of a neoplasm. Our ultrastructural investigation, which demonstrated changes in the mitochondria, further supports the significance of mitochondrial damage in BBS.     

口服川白芷制剂加黑光照射治疗银屑病的电子显微镜观察

本文报告接受口服川白芷制剂加黑光照射治疗的银屑病的皮疹病变超微结构观察。结果显示本疗法效应与文献曾报道过PUVA效应大致相似,其作用是破坏病变细胞,促进基底细胞正常增生发育。     

Recurrent Cervical Lymphadenopathy Due to Ki-1 Lymphoma in an Elderly Female

A 70-year-old female presented with recurring cervical lymphadenopathy over a 5-year period. Intermittently, symptoms of respiratory and Gl tract disease occurred, raising the possibility of malignancy. Investigation of these systems and of the breasts were negative. A lymph node biopsy was eventually done and showed intrasinusoidal proliferations of CD30-posi-tive malignant cells which were also characterized ultrastructurally. Therapy with CHOP has produced a good initial response. The value of electron microscopy in diagnostic pathology is illustrated.     

An Ultrastructural Study of Rabbit Renal Tissues with Experimental Crush Syndrome

Experimental crush syndrome was established in rabbits and the renaltissues were studied with electron microscope.It was found that apartfrom the damages to the renal tubules,the ultrastructural changes of theglomeruli were also prominent,and were characterized by the following:(1)Hypertrophy and hyperplasia of the glomerular cells resulting in nar-rowing and/or obliteration of the capillary loops.(2)Retrogressive changesof the glomerular cells in varying degrees such as mitochondrial swelling,dilatation of endoplasmic reticulum,vacuole formation,etc.On the basisof the morphological findings of the renal tissues in association with theclinical manifestations of acute renal failure,it is proposed that in additionto the damages of the renal tubules,a reduction of the glomerular filtrationrate,which is the result of the altered hemodynamics in the renal vesselsalso plays an important role in the pathogenesis of acute renal failure.