不同喂养方法对烫伤大鼠免疫功能康复影响

目的 观察3种不同喂养方法对烫伤大鼠免疫功能康复影响,探讨促进烧伤机体免疫功能康复方法。方法健康Wistar大鼠100只,随机分成药膳组、肉汤组、常规组(n=30)和对照组(n=10)。药膳组、肉汤组、常规组于伤后3、71、4 d各取10只,正常对照组假伤后立即处死取材,检测T淋巴细胞亚群和自然杀伤细胞(NK)细胞活性、血浆IgAI、gGI、gM、补体C₃、C₄含量、肠道sIgA含量。结果 烧伤药膳组CD₃⁺、CD₄⁺、NK细胞活性分别为(61.87±6.75%)、(33.14±4.91%)、(9.68±1.31%),IgAI、gGI、gM、C₃、C₄及肠道sIgA含量分别为(50.24±8.11)、(197.8±23.2)、(161.9±20.6)、(196.0±24.3)、(73.4±9.4)mg/L和(34.6±8.2)μg/mL,均低于对照组,CD₈⁺([34.33±2.87)%]高于对照组(P<0.05或P<0.01);与肉汤组、常规组比较,药膳组各免疫指标均恢复较快(P<0.05或P<0.01)。结论 药膳喂养可以改善烫伤大鼠T淋巴细胞亚群分布,提高NK细胞活性和肠黏膜细胞sIgA水平,促进机体免疫功能康复。     

检测胃液中sIgA诊断HP感染应用价值

目的:检测胃液sIgA,探讨其在诊断幽门螺杆菌(HP)感染的应用价值。方法:采用放射免疫法检测胃液sIgA并与快速尿素酶法(RUT)、组织涂片染色法、间接ELISA法相比较。结果:HP感染组和非感染组测定sIgA相比较差异有显著性意义(P<0.01)。结论:诊断HP感染时,检测sIgA具有灵敏度高、特异性好、无损伤、简便、易接受等特点,值得在临床上推广。     

理肺通络方对肺炎支原体肺炎大鼠肺泡灌洗液中sIgA的干预作用

目的 观察肺炎支原体肺炎大鼠肺泡灌洗液sIgA的含量及理肺通络方的干预作用。方法 将40只大鼠随机分为正常组、模型组、中药组、西药组，每组10只。一次性滴鼻感染肺炎支原体国际标准株（MPFH）菌液（每毫升含肺炎支原体10^7变色单位）造成肺炎支原体肺炎模型。造模第11 d起，除正常组外，其余各组分别灌服生理盐水、理肺通络方水煎剂、阿齐霉素溶液，2mL／只。中药组连续服药10d，西药组前、后3d灌药液，中间4d灌生理盐水。在治疗的第11d处死动物，观察各组大鼠血清MP—IgM滴度及右肺组织形态学，采用放免双抗夹心法检测sIgA含量。结果 模型组大鼠血清MP—IgM阳性，光镜下显示肺组织炎性细胞浸润和组织损伤明显；与正常组比较，模型组、中药组、西药组肺泡灌洗液中sIgA含量显著降低，有高度统计意义（P〈0．01）；与模型组比较，中药组、西药组sIgA含量显著升高（P〈0．01）；中药组与西药组比较有统计意义（P〈0．05）。结论 肺炎支原体肺炎大鼠肺泡灌洗液sIgA含量下降，理肺通络合剂能提高呼吸道局部sIgA含量，且优于西药阿齐霉素，可能有助于降低再次感染的几率。     

sIgA水平对白念珠菌粘附阴道上皮细胞的影响

目的探讨sIgA在念珠菌性阴道炎(CV)发病中的作用。方法采用放射免疫法测定CV组、健康组及带菌组阴道分泌物中sIgA含量,用体外粘附方法测定白念珠菌对阴道上皮细胞(VEC)的粘附能力,观察sIgA水平时白念珠菌粘附VEC的影响。结果CV组sIgA水平明显低于健康组和带菌组(P<0.01);CV组白念珠菌在含sIgA阴道分泌物中粘附能力高于健康组和带菌组(P<0.05);白念珠菌在不含sIgA阴道分泌物中粘附能力各组间差异无显著性意义(P>0.05);白念珠菌临床株和标准株间差异无显著性意义(P>0.05)。结论sIgA对白念珠菌粘附VEC有抑制作用,sIgA水平的降低是CV发病的原因之一,与白念珠菌来源无关。     

Influence of aging on candidal growth and adhesion regulatory agents in saliva

Although oral candidiasis is frequently seen in the elderly, the factors determining candidal growth have insufficiently been explored. Hence, we examined the influence of aging on candidal adhesion and growth-inhibitory agents in saliva in 45 healthy volunteers and 60 patients with oral candidiasis. Both non-stimulated and stimulated salivary flow rates (SFRs) in the healthy controls decreased with aging. A gradual decrease of SFRs with aging was also observed in the patients, and the SFR levels were markedly lower than those in the controls. Although the salivary glucose levels were almost constant in all age groups, secretory immunoglobulin A and lactoferrin levels in saliva were significantly decreased statistically with age, and a marginal age-associated decrease in transferrin levels was also observed. In addition, the generation of superoxide from neutrophils in saliva and their Candida killing activity decreased with age, and these phenomena were more apparent in the patients. Furthermore, a larger number of Candida adhered to oral keratinocytes obtained from the elderly healthy controls than to those obtained from young controls. Correspondingly, keratinocytes from the aged controls showed more concanavalin-A binding sites than those from the young controls. However, oral Candida did not increase with increasing age in the controls, although an age-associated increase of oral Candida was observed in the patients. Taken together, these results indicate that the decreases of SFRs and salivary anti-candidal factors, suppression of salivary neutrophil function and the increase of candidal adhesion sites on keratinocytes predispose elderly individuals to oral candidiasis.     

温肺通窍方对儿童肺气虚寒型过敏性鼻炎黏膜免疫影响的临床研究

[目的]通过观察温肺通窍方治疗肺气虚寒型过敏性鼻炎患儿的临床疗效,探索温肺通窍方对此类患儿局部慢性炎症及黏膜免疫的影响.[方法]将84例肺气虚寒型过敏性鼻炎患儿随机分为试验组和对照组,每组各42例.试验组给予温肺通窍方治疗,对照组给予氯雷他定片治疗,疗程为7 d.观察2组患儿治疗前后鼻呼气一氧化氮(FeNO)浓度和鼻腔...     

Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects

The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated. C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days. Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA). Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum. Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS. Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control. Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group. HGB intake increased the bacterial count of microbiota, such as Bifidobacterium and Lactobacillus. Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level. Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet. Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.     

白色念珠菌二相性与口腔致病性关系的实验研究

目的　探讨白色念珠菌二相性与口腔致病性的关系。方法　分别将孢子相、菌丝相白色念珠菌与人口腔颊粘膜细胞 (BEC)进行粘附试验 ,比较二相性白色念珠菌对BEC的粘附率 ;用兔抗人sIgA血清中和人唾液中sIgA ,12 5Ⅰ sIgA放射免疫分析法检测唾液中sIgA的浓度 ,比较含sIgA及不含sIgA的唾液对二相性白色念珠菌粘附BEC的影响。 结果　菌丝相白色念珠菌对BEC的粘附率显著高于孢子相 (P <0 .0 0 1) ;含sIgA的唾液抑制孢子相及菌丝相白色念珠菌粘附BEC的能力强于不含sIgA的唾液 (P <0 .0 0 5 ) ,且唾液中sIgA抑制孢子相白色念珠菌粘附BEC的能力强于抑制菌丝相白色念珠菌粘附BEC的能力 (P <0 .0 5 )。结论　菌丝相白色念珠菌的口腔致病性强于孢子相     

Modulation of intestinal mucosal immunity by dietary polyunsaturated fatty acids in chickens

This study was undertaken to investigate the effects of dietary polyunsaturated fatty acids (PUFA) on intestinal immune responses and cytokines secretions in chickens at nutrition-immunity level. The proportion of CD3⁺CD8⁺ lymphocytes of intestinal mucosa in CO group was higher on d 21 than that in FO (p?<?0.01) and PO (p?<?0.05) group. The proportion of CD3⁺CD4⁺ lymphocytes of FO group was greatly significantly higher on d 21, relative to PO or CO group. The FO treatment enhanced the sIgA concentration in jejunum on d 42, compared with CO and PO group. Fish oil and corn oil had similar effects on enhancing IL-2 secretion of PBMC, and converse effects on IL-4 secretion. Compared with PO group, chickens supplemented with fish oil or corn oil had greater concentration of intestinal mucosal membrane phospholipids on d 21 and d 42. In conclusion, the changes of intestinal lymphocytes membrane phospholipids in FO or CO group would modulate lipid second messengers. The changes of CD expression would affect sIgA secretion of intestinal lymphocytes. Diet supplemented with corn oil or fish oil can affect cytokines secretion of PBMC in chickens, which works on systemic and mucosal immune response.     

肠黏膜免疫:刚地弓形虫感染中抗体分泌细胞及sIgA的地位

病原体经口感染机体后,肠相关淋巴组织受刺激,促进肠黏膜内IgA抗体分泌细胞(IgAASC)的形成,并向肠腔分泌大量的分泌型免疫球蛋白A(sIgA),IgA ASC和sIgA在肠黏膜免疫应答中起了重要作用.BALB/c小鼠经口感染弓形虫RH速殖子后,小鼠小肠黏膜固有层IgA ASC大量增殖,小肠冲洗液sIgA水平持续增高,发挥抗虫效应.小肠冲洗液sIgA水平与十二指肠黏膜IgA ASC数量呈正相关(r＝0.732,P<0.01).用可溶性速殖子抗原(STAg)鼻内免疫小鼠可诱导卜二指肠黏膜固有层IsAASC高表达,小肠液和血清sIgA水平升高,表明在抗弓形虫感染中STAg鼻内免疫发挥了作用.