白血病细胞p16基因突变分析

目的探讨ｐ１６基因突变在白血病发生中的作用及基因突变的机制。方法利用点突变检测仪、水平和垂直板电泳对ｐ１６基因的外显子１、外显子２的ＰＣＲ扩增产物作缺失和点突变分析。结果在白血病３５例临床标本中有２２例发生缺失突变，６例发生点突变，突变率约８０％。在２２例缺失突变病例中，有１０例为不完全缺失突变即有低于外显子５０９ｂｐ的扩增产物。结论ｐ１６基因含有“ＧＣ”ＤＮＡ重复顺序，易发生ＤＮＡ重组及易位和重排。在白血病发生中起重要作用     

三尖杉酯碱诱导慢性髓系白血病K-562细胞凋亡的实验研究

目的阐明三尖杉酯碱（HT）作用于K562细胞的机制。方法应用细胞形态、DNA凝胶电泳和流式细胞仪等检测,观察HT对K562细胞株的作用方式,进一步用RT-PCR方法研究bcr/abl基因在转录水平的改变。结果0.01～100.00μg/ml浓度HT可诱导K562细胞凋亡,并呈时间和剂量依赖性。随着药物作用时间的延长,bcr/abl基因的转录下调。结论低浓度HT就可通过抑制bcr/abl基因的表达,诱导K562细胞凋亡。     

不同锑剂对早幼粒细胞白血病凋亡诱导作用比较

目的：通过不同锑剂对早幼粒白血病细胞株NB4细胞的凋亡诱导作用的比较，力求找到有效治疗早幼粒细胞白血病锑剂药物。方法：采用细胞生长曲线、形态学及NBT（硝基四氮唑蓝）还原试验判定NB4细胞的生长、分化及功能；细胞周期分析和DNA电泳研究细胞凋亡。结果：三价锑剂能够诱导早幼粒白血病细胞凋亡，且具有时间剂量依赖性，而五价锑剂对早幼粒白血病细胞没有凋亡诱导作用。结论：三价锑剂能够有效地诱导早幼粒白血病细胞凋亡，提示酒石酸锑钾作为临床治疗寄生虫病有效药物，可以老药新用，有望用于临床治疗早幼粒细胞白血病。     

Granulocytic sarcoma: An atypical presentation in the oral cavity

Acute myelogenous leukemia (AMU is a hematologic disorder that is characterized by an abnormal proliferation of immature myeloid cells. Granulocytic sarcomas are clusters of leukemic myeloid cells that may develop as a result of AML. Oral manifestations of AML are common and often involve enlargements of the gingiva and/or mucosal tissue from direct leukemia cell infiltration. We describe the case history of a 50-year-old man who had an ulcera-tive lesion of the oral mucosa that was determined to be a granulocytic sarcoma of AML-M0 subtype. The combination of both the subtype and clinical presentation of the leukemia makes this presentation unusual, and to the best of our knowledge, of a type that has not been previously reported in the literature.     

冬凌草甲素对Raji细胞的增殖抑制作用及其机制

目的探讨冬凌草甲素对B淋巴细胞白血病细胞株Raji细胞的增殖抑制作用及其机制。方法以不同浓度的冬凌草甲素作用于体外培养的Raji细胞，MTT法检测细胞生长抑制率，应用流式细胞仪、Hoechst 33258荧光染色法观察细胞凋亡，TRAP-PCR-ELISA法检测细胞凋亡前后的端粒酶活性。结果冬凌草甲素可显著降低Raji细胞的端粒酶活性，抑制细胞的生长，诱导细胞发生凋亡，并呈现出明显的剂量-效应与时间-效应关系。结论冬凌草甲素对B淋巴细胞白血病具有明显的体外抗白血病作用，降低细胞端粒酶活性，诱导细胞发生凋亡，可能是其重要作用机制。     

Therapeutic effect of 5-FC on YCD modified murine P388 leukemia in vivo

Cytosine deaminases (CDs) in bacteria andfungi are found to deaminate prodrug 5 - FC intocytotoxic agent 5 - FU .Yeast CD (YCD) is clonedfrom Saccharomyces cerevisiae.It has been shownpreviously that YCD was more efficient inconversing5 - FC than bacterial CD(b CD) [1-3 ] .As anovel suicide gene therapy system,YCD/ 5 - Fcsystem may be promising in cancer therapy andprevention of graft versus host disease.In thepresent study,we established a P388/ DBA murineleukemia model by infusin…