中学生考试焦虑症患者认知功能损害的事件相关电位P300

目的探讨中学生考试焦虑症患者认知功能的事件相关电位P300特征。方法将30例诊断为考试焦虑症中学生患者作为实验组,与实验组性别、年龄匹配的30例健康中学生作为对照组。对照组、实验组在考试前后分别予P300检测及Zung氏焦虑自评量表（SAS）评定,比较组间P300各成分及SAS评分的差异。结果与对照组相比,考试前实验组P3潜伏期及反应时间明显延长,P3波幅明显降低（t=6．823～9．051,P〈0．05或0．01）。考试后,实验组P3潜伏期及反应时间比考试前明显缩短,P3波幅明显增高（t=5．973—11．120,P〈0．05或0．01）;考试前实验组SAS评分与P3潜伏期、反应时间呈正相关关系,与波幅呈负相关关系（r=0．4,0．8,-0．51,P〈O．05或0．01）。结论中学生考试焦虑症患者存在认知功能损害,针对缓解焦虑症状的干预十分必要。     

人工软骨材料——聚乙烯醇水凝胶的研制

聚乙烯醇溶液于－２０℃左右的温度下冷冻６－１２ｈ,室温下颌化１－２ｈ,上述过程反复进行１－３次,然后对试样进行真空脱水处理,制得一种人工软骨材料－ＰＶＡ水凝胶。     

Laparoscopic electrocautery of the ovaries versus recombinant FSH in clomiphene citrate-resistant polycystic ovary syndrome. Impact on women's health-related quality of life

BACKGROUND: Ovulation induction with gonadotrophins is the standard treatment strategy for women with clomiphene citrate (CC)-resistant polycystic ovary syndrome (PCOS). Laparoscopic electrocautery of the ovaries is an alternative treatment modality, leading to a comparable cumulative pregnancy rate. In deciding which treatment to opt for, women's health-related quality of life (HRQoL) should be taken into account. METHODS: A total of 168 CC-resistant women with PCOS were randomly assigned to receive either the electrocautery strategy, entailing laparoscopic electrocautery of the ovaries followed by CC and recombinant FSH (rFSH) if anovulation persisted, or ovulation induction with rFSH. We assessed women's HRQoL with the standard questionnaires Short Form-36, Rotterdam Symptom Checklist and Center for Epidemiological Studies Depression Scale, administered before randomization and 2, 12 and 24 weeks thereafter. RESULTS: The intention to treat analysis revealed no significant differences between the treatment groups on any of the scales at any point during follow-up. In women without an ongoing pregnancy, those treated with rFSH showed significantly more depressive symptoms than women allocated to the electrocautery strategy, with or without CC, although differences were small. CONCLUSIONS: Overall, HRQoL was not affected in both groups. In women still under treatment, rFSH was slightly more burdensome for women's HRQoL than electrocautery with or without CC.     

免疫性输血反应的调查及预防研究

目的　对各类输血反应进行调查研究 ,同时建立输血前免疫血液学检查新技术 ,以预防和治疗免疫性输血反应。方法　对上海地区 13家医院的 30 776名输血病人进行调查 ,并应用改良Polybrene、简易致敏红细胞血小板血清学试验 (SEPSA)等新技术 ,进行同种特异性抗体的筛选、鉴定及交叉配型试验。结果　发生各类输血反应5 0 8例 (1.6 5 % ) ,其中检出引起免疫性输血反应的同种抗体 6 4例 (12 .6 % ) ,抗体特异性分别为血小板抗体 (包括HLA) 5 1例 ,红细胞不规则抗体 13例 ;各项新技术的应用对预防免疫性输血反应的发生具有显著相关性 (χ2 >3.84 ,P <0 .0 5 )。结论　使用研究建立的输血前免疫血液学新技术能有效地诊断、预防和治疗免疫性输血反应的发生 ,提高了输血的安全性和有效性     

转移性肝癌多弹头射频治疗的疗效观察及价值

目的：探讨超声引导经皮肝穿治疗转移性肝癌的疗效及价值。方法：对18例转移性肝癌22次射频治疗,观察治疗前后肿瘤大小。结果：18例于治疗后10天,16例60天、13例6个月、5例12个月进行B超及CT复查,结果：10天后80％病人瘤体缩小30％以上,6个月后瘤体缩小40％,5例已存活1年,90％的病人自觉症状改善,无手术死亡,结论：超声引导经皮穿刺射频治疗转移性肝癌杀伤肿瘤彻底,对机体损害小,疗效确切而满意,对临床有较高的应用价值。     

Effectiveness of washed platelet concentrate and red cell transfusions for a patient with anhaptoglobinemia with antihaptoglobin antibody

A 71-year-old Japanese male with myelodysplastic syndrome progressing to overt leukaemia and hepatocellular carcinoma developed dyspnea and urticaria immediately after infusion of platelet concentrate (PC). He exhibited an identical reaction following blood transfusion. Serum haptoglobin was undetectable. The patient was determined to be homozygous for Hp(del) by polymerase chain reaction (PCR). Antibody to haptoglobin was detected by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. No antibodies against human leucocyte antigen (HLA) or platelet-specific antigens were detected. Washed PC and washed red blood cells were effective in preventing the transfusion-related anaphylactoid reactions.     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

丹参注射液诱导大鼠骨髓间充质干细胞分化为神经元样细胞的实验研究

目的:探讨丹参注射液在体外诱导大鼠骨髓间充质干细胞(rMSCs)向神经元样细胞分化的作用.方法:采用贴壁筛选法分离rMSCs,并通过不断传代进行纯化和扩增培养.采用含丹参注射液的无血清Dulbecco改良Eagle培养基(DMEM)/F-12培养液诱导rMSCs分化为神经元样细胞,观察不同浓度丹参注射液对rMSCs诱导分化为神经元样细胞的影响.通过观察rMSCs经诱导后细胞的形态变化和采用免疫组化方法检测神经元特异性核蛋白(NeuN)、神经丝蛋白(NF-M)、胶质纤维酸性蛋白(GFAP)的表达来鉴定神经元样细胞.结果:经丹参注射液诱导后,rMSCs胞体收缩,突起伸出,形似神经元;免疫组化显示诱导出的神经元样细胞NeuN、NF-M表达阳性,GFAP阴性.丹参注射液在浓度为10 ml/L时的诱导作用最为显著.结论:丹参注射液可以在体外诱导rMSCs分化为神经元样细胞,其诱导分化率与丹参注射液的浓度有关.     

体外循环术后TEG监测值与胸腔引流量和输血量的相关性研究

目的 通过血栓弹力描记图（TEG）的监测和传统凝血功能监测了解体外循环后凝血功能的改变，比较两者与术后出血量（胸腔引流量）和输血量的相关性，评估TEG在体外循环手术中的监测意义。 方法 124例病人分别在术前、肝素中和后10min、肝素中和后的3h抽血监测TEG和凝血三项（凝血酶原时间：PT，活化全血凝固时间：aPTT；纤维蛋白原：Fb）。记录病人回ICU后不同时间点的引流量以及病人总的输血量。比较病人在体外循环后凝血功能的变化，并分别作肝素中和后10min时的监测结果与不同时间点引流量和总输血量的相关性分析。 结果 ①病人的凝血功能在体外循环后都受到了明显的损害（P〈0．01）；②病人的引流量、输血量和监测值有一定的相关性，而TEG的监测值比凝血三项相关性好。 结论 TEG和凝血三项都能反映出体外循环后病人凝血功能的损伤，而TEG与术后的出血量及输血量的相关性更好，能对临床治疗起到有效的监测和一定的指导作用。