Use of Cell Fat Mixed with Platelet Gel in Progressive Hemifacial Atrophy

Progressive hemifacial atrophy, also known as Parry-Romberg syndrome, is an uncommon degenerative and poorly understood condition. It is characterized by a slow and progressive atrophy affecting one side of the face. The incidence and cause of this alteration are unknown, and the pathogenesis of the syndrome is not clear. Some authors attribute the atrophy of the subcutaneous system to an alteration of the sympathetic system. Others attribute it to an alteration of the nervous system at the encephalic level or to an interstitial neuritis of the trigeminal nerve. The most common complications that appear in association with this disorder are trigeminal neuritis, facial disorders, and epilepsy. The latter is the most frequent complication of the central nervous system. Characteristically, the atrophy progresses slowly for several years and soon becomes stable. After stabilization of the disease, plastic surgery of autogenous fat grafts can be performed. This study aimed through the presentation of clinical cases to suggest a therapeutic plan comprised of two sequential treatments: aquisition of platelet gel from a small volume of blood (9 ml) followed by the Coleman technique for reconstructing the three-dimensional projection of the face contour, restoring the superficial density of the facial tissues. The results obtained prove the efficacy of these two treatments combined, and the satisfaction of the patient confirms the quality of the results.     

Associations of serum EBV DNA and gammopathy with post-transplant lymphoproliferative disease

Abstract: Background: Post‐transplant lymphoproliferative disease (PTLD) is a life‐threatening complication of immunosuppression following transplantation. Epstein–Barr virus (EBV) and gammopathy in serum are associated with PTLD, but these two parameters have not been evaluated in parallel for their association with PTLD. Methods: We evaluated the incidence of EBV load positivity, gammopathy, and protein expression in sera from all PTLD patients diagnosed at our hospital during the past seven yr. Results were compared with those of a control group including matched transplanted patients who did not develop PTLD. Results: Seven of 10 PTLD patients presented EBV⁺ PTLD, for which five patients had detectable serum EBV DNA levels compared with none of 38 controls (RR between two groups =121, p < 0.0001). Five out of 10 patients had gammopathy at PTLD diagnosis compared with 5/38 controls (RR between two groups = 6.6, p = 0.022). Additionally, protein serum analysis by high‐resolution two‐dimensional gel electrophoresis and image examination failed to evidence specific abnormality in patients with PTLD compared with controls. Conclusions: Our results confirm an association between EBV in sera and gammopathy with PTLD, and highlight the high specificity of the former analysis. Whether a combination of both analyses will improve the clinical detection of PTLD remains to be evaluated in a larger prospective cohort study.     

皮肤软组织扩张器联合硅凝胶瘢痕贴片在烧伤瘢痕整形修复中的应用与研究

分析整形修复烧伤瘢痕中运用皮肤软组织扩张器与硅凝胶瘢痕贴片联合治疗的效果。方法 选择2021年6月-2022年6月广州市花都区人民医院收治的60例烧伤瘢痕患者作为研究对象,采用随机数字表法划分为对照组、试验组,每组30例。对照组予以皮肤软组织扩张器治疗,试验组予以皮肤软组织扩张器联合硅凝胶瘢痕贴片治疗,比较两组创面愈合情况、修复效果。结果 试验组创面皮肤恢复血运时间及愈合时间短于对照组,皮肤扩张率高于对照组,术后皮肤面积及Ⅰ型/Ⅲ型胶原比例优于对照组,感染率低于对照组,差异有统计学意义（P ＜0.05）;两组治疗后弹性、质地、外观评分均高于治疗前,且试验组弹性、质地、外观评分高于对照组,差异有统计学意义（P ＜0.05）。结论 整形修复烧伤瘢痕中采取皮肤软组织扩张器与硅凝胶瘢痕贴片联合治疗可加快瘢痕的恢复速度,保护患者肌肤的美观性,促进患者的身心健康,临床疗效良好。     

苯对人精子DNA损伤的影响

目的: 研究苯是否引起接触工人精子DNA损伤。　方法: 选择 2年以上苯系物作业工人 27例为暴露组,无苯系物接触史者 35例为对照组,采用高压液相色谱法测定空气苯系物浓度;研究对象尿中反式粘糠酸浓度。采用单细胞凝胶电泳技术(SCGE)检测部分工人 (暴露组 13例,对照组 16例 )精子DNA损伤。　结果: 作业点苯浓度为(86. 49±2. 83)mg/m3,高于国家标准(40mg/cm3 )1倍以上;暴露组尿中反式粘糠酸平均浓度为 (1. 40±0. 62)mg/L,对照组为(0. 82±0. 16 )mg/L,P<0. 01;以DNA头部百分比为检测参数,SCGE结果显示,暴露组为 ( 70. 18±7. 36)%,明显低于对照组(90. 62±2. 94)%,P<0. 001。　结论: 苯可以引起精子DNA断裂损伤。     

Proteome analysis of round-headed and normal spermatozoa by 2-D fluorescence difference gel electrophoresis and mass spectrometry

Globozoospermia is a severe form of teratozoospermia characterized by round-headed spermatozoa with an absent acrosome, an aberrant nuclear membrane and midpiece defects. Globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa on semen analysis, and patients with this condition are absolutely infertile. The objective of this study was to investigate the differences in protein expression between human round- headed and normal spermatozoa. Two-dimensional （2-D） fluorescence difference gel electrophoresis （DIGE） coupled with mass spectrometry （MS） was used in this study. Over 61 protein spots were analysed in each paired normal/round-headed comparison, using DIGE technology along with an internal standard. In total, 35 protein spots identified by tandem mass spectrometry （MS/MS） exhibited significant changes （paired t-test, P 〈 0.05） in the expression level between normal and round-headed spermatozoa. A total of nine proteins were found to be upregulated and 26 proteins were found to be downregulated in round-headed spermatozoa compared with normal spermatozoa. The differentially expressed proteins that we identified may have important roles in a variety of cellular processes and structures, including spermatogenesis, cell skeleton, metabolism and spermatozoa motility.     

腹泻病原菌中产超广谱β-内酰胺酶菌株的基因型和同源性分析

目的了解分离的产超广谱β-内酰胺酶(ESBLs)腹泻病原菌的基因流行情况。方法采用TEM型、SHV型和CTX-M型ESBLs编码基因的通用引物对产ESBLs菌株进行扩增,并采用PFGE技术对分离的产ESBLs腹泻病原菌进行了DNA指纹图谱分析。结果在分离的产ESBLs的腹泻病原菌中,CTX-M型是最为常见的ESBLs型别,TEM型次之,SHV型最少,CTX-M亚型中CTX-M-14最多,部分产ESBLs腹泻病原菌同时产生一种以上的β-内酰胺酶,少数菌株的ESBLs基因型不属于上述三种基因型。分离的产ESBLs的大肠杆菌有6种基因型,宋内菌有2种基因型,变形杆菌和克雷伯菌只有1种基因型。结论 ESBLs不仅可以存在于不同种的细菌中,也可以存在同一种细菌的不同基因型中,推测这可能是耐药基因在不同细菌或同一细菌不同基因型中平行转移的结果。     

凝胶色谱法测定注射用美洛西林钠舒巴坦钠中聚合物

目的建立一种利用葡聚糖凝胶柱分离测定注射用美洛西林钠舒巴坦钠中美洛西林钠聚合物的方法。方法Sephadex G-10色谱柱,流动相A为0.05 mol·L-1磷酸盐缓冲液（pH 7.0）,流动相B为超纯水,流速为1.0 ml·min-1,检测波长为254 nm,进样量200μl。结果美洛西林对照品在20.2l～60.6μg·ml-1范围内与其峰面积成良好线性关系,r=0.9992。结论本方法简便、准确、重复性好,适用于注射用美洛西林钠舒巴坦钠聚合物的定量检测。     

ADPRT抑制剂对人肿瘤细胞的放射增敏效应

目的：从细胞的克隆形成能力和细胞ＤＮＡ双链断裂及修复几方面探讨了ＡＤＰ－核糖基转移酶（ＡＤＰＲＴ）的特异性抑制剂３－氨基苯甲酰胺（３－ＡＢ）对人卵巢癌细胞株ＨＯＣ８的放射增敏效应。结果表明，３－ＡＢ能降低受照细胞的克隆形成能力；照射所诱发的初始ＤＮＡ双链断裂水平不受３－ＡＢ的影响，但细胞对双链断裂的修复能力受到抑制，表现为慢速修复水平下降，两方面的结果呈正相关。结论：通过脉冲电场凝胶电泳测定ＤＮＡ双链断裂及其修复水平，可以预测细胞的放射敏感性。     

聚丙烯酰胺水凝胶注射后不良反应的剖析

目的 研究分析聚丙烯酰胺水凝胶填充物被迫取出者的症状体征和局部病理变化。方法 从2000年1月～2001年12月，我们对15例在外院行聚丙烯酰胺水凝胶注射充填者行充填物取除术。对不良反应的主要症状、体征和切除物的病理变化行统计学分析。结果 主要症状体征中：无美容效果占33.33％，继发畸形占20.00％，局部疼痛占60.00％，局部不适占13.33％，局部硬结占80.00％，局部红肿占6．67％。切除组织的主要病理改变：包膜形成占53.33％，异物巨细胞反应占60．00％，肉芽肿占20.00％。结论 聚丙烯酰胺水凝胶注入体内后会出现一定程度的不良反应，对其临床应用应持谨慎态度。     

上海市宝山区2015—2018年腹泻病人沙门菌血清分型和分子分型

目的 揭示上海市宝山区腹泻病人分离沙门菌的血清型和分子分型分布特征。方法 对2015—2018年分离的118株沙门菌进行血清分型和脉冲场凝胶电泳（pulsed-field gel electrophoresis,PFGE）分型,通过构建聚类树揭示不同血清型菌株的分子分型特征。结果 118株沙门菌分为25种血清型,前三位为肠炎沙门菌（45株,38.1%）、鼠伤寒沙门菌（26株,22.0%）、伦敦沙门菌（11株,9.3%）。其余22种血清型分别只包含1~3株菌。118株沙门菌通过PFGE分为71个PFGE带型,每个带型分别包含1~19株菌。45株肠炎沙门菌分为8个PFGE带型,相似度为75.0%~100.0%,在聚类树上形成一个簇;26株鼠伤寒沙门菌分为21个PFGE带型,相似度为48.0%~100.0%,在聚类树上形成两个簇;11株伦敦沙门菌分为8个PFGE带型,相似度为80.0%~100.0%,在聚类树上形成一个簇。结论 肠炎沙门菌和鼠伤寒沙门菌是上海市宝山区腹泻病人沙门菌优势血清型,但近年伦敦沙门菌有增多的趋势。肠炎沙门菌在本地区传播流行过程中的遗传变异相对比较保守,鼠伤寒沙门菌基因型多态性大,而且存在两个克隆。