骨髓基质干细胞移植对大鼠胶质瘤局部微环境的影响

目的:探讨骨髓基质干细胞(BMSCs)移植对脑胶质瘤模型大鼠(荷瘤大鼠)胶质瘤的影响。方法:观察BMSCs培养上清液对C6胶质瘤细胞增殖活性、细胞周期的影响。观察脑内移植BMSCs对胶质瘤核增殖抗原(PCNA)表达阳性率、脑内胶质瘤微血管计数的影响及荷瘤鼠生存期的改变。结果:BMSCs培养上清液对胶质瘤细胞增殖没有明显的影响;BMSCs可抑制肿瘤边缘及卫星灶的肿瘤增殖,对肿瘤内部的细胞增殖没有明显影响。移植BMSCs后肿瘤边缘及卫星灶的微血管计数未见明显改变。移植BMSCs后的荷瘤大鼠脑内胶质瘤水肿有所减轻。结论:BMSCs移植可轻度抑制荷瘤鼠脑内胶质瘤细胞的增殖。     

Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: IV modulation of membrane fluidity of leukemic cell lines and tonsillar cell stimulated with McAbs

Summary In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the modulation effect on membrane-fluidity induced by McAbs against isotypic and idiotypic determinants of IgM from patients with leukemia was studied as well. The expression of the corresponding isotypic and idiotypic determinants of IgM on the cell membrane was determined. The results show that the membrane fluidity of leukemic cell lines is remarkably higher than that of tonsillar cells from healthy persons, and McAbs against isotypic determinants of leukemic IgM can enhance the membrane fluidity of all kinds of cells mentioned above. However, the anti-idiotypic monoclonal antibody increased only the membrane fluidity of leukemic cell lines. These results indicated that there was a close relationship between the effect of McAbs on cell membrane fluidity and the expression of corresponding isotypic and idiotypic determinants of IgM on the cell membrane.     

Measurement of arterial input functions for dynamic susceptibility contrast magnetic resonance imaging using echoplanar images: comparison of physical simulations with in vivo results.

Measurement of the arterial input bolus shape is essential to the quantification of mean transit time and blood flow with dynamic susceptibility contrast (DSC) MRI. Input functions derived from the echoplanar signal intensity within or near arteries are highly nonlinear, yet such input functions are widely used. We employed a physical model for the echoplanar signal intensity from an artery as a function of contrast agent concentration, artery size, and angle to the magnetic field to test approaches for the measurement of the arterial input function. The simulated results confirmed the strong nonlinearity of signal in the neighborhood of vessels. Of the input function measurement methods considered, the simulations suggested that measurement of signal near but not within a large vessel is most accurate, but mean transit times (MTT) calculated with these input functions are highly sensitive to peak bolus concentration. Input functions determined from voxels demonstrating the shortest first moment overestimated the MTT but the measured MTTs were more robust to changes in peak concentration. Characteristics of the measured in vivo input functions were consistent with the simulations. Our results emphasize the important contribution of input function errors to the uncertainty in MTT and blood flow imaging with DSC MRI.     

包头市区400名健康成年人发硒值测定

报告用巯基棉（ＳＣＦ）吸附富集分离──催化动力学法，测定了包头市区４００名１８～５５岁健康成年人发硒值。结果显示：发硒值的中位数（Ｍ）为０．７６ｍｇ／ｋｇ。男女分组分析，男性的Ｍ为０．６９ｍｇ／ｋｇ，女性Ｍ为０．８０ｍｇ／ｋｇ，男女两组间的差异无显著意义（Ｐ＞０．０５）。     

增殖细胞核抗原(PCNA)与胃癌预后关系的免疫组化研究

应用ＳＬＡＢ免疫酶标法在石蜡切片上半定量分析增殖细胞核抗原（ＰＣＮＡ）的表达与７３例胃癌患者预后的关系，探讨其临床应用价值。结果表明，以ＰＣＮＡ阳性细胞５０％为界线，阳性率＞５０％者术后生存期较短，５年生存率较低，而淋巴结转移率则较高。提示ＰＣＮＡ检测可作为胃癌预后判断的良好指征。     

595nm激光对兔耳瘢痕成纤维细胞增殖与凋亡的影响

目的：探讨595nmVbeam激光照射对增生性瘢痕动物模型伤口愈合过程中成纤维细胞增殖与凋亡的影响。方法：成年大耳白兔20只,建立兔耳腹侧面增生性瘢痕模型,对比研究在瘢痕形成过程中595nmVbeam激光照射对兔耳瘢痕成纤维细胞的影响,采用免疫组化方法检测增殖细胞核抗原（PCNA）蛋白表达和细胞凋亡的原位检测。结果：兔耳增生性瘢痕经595nmVbeam激光照射后,按不同时间段取材进行免疫组化染色并与对照组比较,高倍镜下观察结果,显示PCNA蛋白表达明显减弱,细胞凋亡增加。结论：595nmVbeam激光照射可抑制兔耳增生性瘢痕成纤维细胞的增殖过程,诱导细胞凋亡。应用595nmVbeam激光预防和治疗瘢痕是可行的。     

Ki-67、增殖细胞核抗原、P53在脑膜瘤中的表达及意义

目的 :通过对 Ki- 6 7、增殖细胞核抗原、P5 3在脑膜瘤中的表达 ,进一步研究其与肿瘤的良、恶性及肿瘤复发的关系 ,从而更好判断其生物学行为。方法 :行脑膜瘤全切除手术治疗 ( Simpson's Grade or Grade )并经 9～12 4个月 ( 4 8± 9)随访的 6 0例 ,采用 SP免疫组化法 ,检测手术切除石蜡包埋的肿瘤标本的 Ki- 6 7、增殖细胞核抗原、P5 3抗原 ,以显微镜下的细胞核内出现棕色颗粒为阳性 ,计算 3者的标记指数 ( L abeling Index) .对患者的临床资料与肿瘤细胞的标记指数的差异性进行统计学分析。结果 :Ki- 6 7、增殖细胞核抗原、P5 3在不同病理形态脑膜瘤中的表达具有显著性差异 ( P<0 .0 5 ) ,恶性脑膜瘤、非典型性脑膜瘤中的表达明显高于良性脑膜瘤 ;同样在复发性脑膜瘤中其表达也明显高于非复发性脑膜瘤 ( P<0 .0 5 ) ;良性脑膜瘤中 Ki- 6 7、增殖细胞核抗原的表达在复发组明显高于非复发组 ( P<0 .0 5 ) ,而 P5 3的表达在复发组与非复发组无统计学意义 ( P>0 .0 5 )。结论 :( 1) Ki- 6 7、增殖细胞核抗原、P5 3的表达与病理分型和脑膜瘤术后复发有关。 ( 2 )良性脑膜瘤结合 Ki- 6 7、增殖细胞核抗原的表达情况可用来判断预后即有无复发的可能 ,从而进一步来指导临床治疗     

大鼠肝癌发生发展过程中肝组织增殖细胞核抗原动态演变规律

目的研究原发性肝细胞癌发生、发展过程中肝组织增殖细胞核抗原(proliferatingcellnuclearanti-gen,PCNA)的动态演变规律。方法将60只SD大鼠按随机数字表法随机分为对照组(20只)和实验组(40只)。用化学致癌剂3,3′-二氨基联苯胺(3,3′-diaminobenzidine,DAB)诱发SD大鼠肝癌,采用免疫组织化学方法连续监测各时相肝组织中PCNA的表达,光镜下观察肝组织的病理变化。结果本实验的诱癌过程可分为炎性变期、增生纤维化期和癌变期3个阶段;PCNA的表达最早出现于炎性变早期汇管区的卵圆细胞中,并在汇管区呈区域性过表达分布;炎性变中期,PCNA表达阳性率达高峰,随后下降,在癌变期又升高,在整个病理演变过程中其表达具波动性。结论在致癌因素的存在下,PCNA首先表达于卵圆细胞;PCNA表达的动态演变对肝癌的发生有一定的预警作用。     

离子型与非离子型MR对比剂对肝脏病变的增强效果比较

目的　对比离子型钆喷酸葡胺 (Gd -DTPA)和非离子型钆双胺MR对比剂 (Gd -DTPA -BMA)在肝脏病变手推团注多时相增强扫描中的增强效果。方法　 5 8例肝脏疾病患者 (男 3 1例 ,女 2 7例 ,年龄 3 0～ 77岁 )分 2组 ,第 1组 :3 0例 (男 16例 ,女 14例 ) ,采用Gd -DTPA -BMA。第 2组 :2 8例 (男 17例 ,女 11例 ) ,采用Gd -DTPA。行MR手推团注多时相增强扫描。结果　Gd -DTPA -BMA组 :增强效果满意者 2 3例 (77% ) ,增强效果一般者 6例 (2 0 % ) ,增强效果差者 1例 (3 % )。Gd -DTPA组 :增强效果满意者 2 2例(79% ) ,增强效果一般者 6例 (2 1% )。结论　在肝脏疾病MR手推团注多时相增强扫描中 ,Gd -DTPA -BMA和Gd -DTPA均可达到满意的增强效果 ,安全及有效性两者无显著性差异。Gd -DTPA -BMA粘滞度小 ,利于快速推注。     

CMSS-VEPs: Contrast modulated steady state visual evoked potentials: Its neuronal origin and clinical use

CMSS-VEPs are presented as a sensitive, non-invasive functional investigation technique of the visual function, applicable in clinical practice. New improvements of the technique are presented. The underlying neuronal mechanisms are discussed. The clinical use is illustrated in a case of optic neuritis.