增强型绿色荧光蛋白基因转染及TGFβ3诱导前软骨干细胞向成软骨方向分化

目的　探讨增强型绿色荧光蛋白基因 (EGFP)转染前软骨干细胞 (PSCs)的效果及TGFβ3 诱导PSCs向成软骨方向定向分化的可行性。方法　利用磁性细胞分选系统分离纯化有成纤维细胞生长因子受体 3(FGFR 3)表面标志的PSCs。采用脂质体介导法将EGFP基因转染PSCs,G4 18筛选得到EGFP基因修饰PSCs。用免疫组化及免疫荧光检测EGFP基因修饰PSCs的FGFR 3表达。采用藻酸盐凝胶培养 ,以TGFβ3 诱导分离纯化的PSCs向成软骨方向定向分化。应用免疫组化、RT PCR检测PSCs向成软骨方向分化过程中特异性软骨基质成分的表达情况。结果　EGFP基因转染PSCs后 ,2 4hGFP开始表达 ,4 8～ 72hGFP表达最强。G4 18筛选后 ,EGFP基因修饰PSCs体外培养 6周仍有较强GFP表达。在含TGFβ3 的藻酸盐凝胶培养 7、14、2 1d均有II型胶原表达 ;诱导2 1d的PSCs免疫组化检测可见细胞及周围均有X型胶原、Aggrecan、COMP表达 ;RT PCR检测显示在培养早期 ( 8d内 )开始出现Aggrecan、X型胶原、COMP等的表达 ,中期 ( 8d后...     

Finger-assisted stretching technique for cesarean section.

OBJECTIVE: To compare the perioperative outcomes of two cesarean section methods, the finger-assisted stretching technique (FAST), based on a modified Joel-Cohen method, with the traditional technique. METHODS: A retrospective review of the records of 416 women who underwent cesarean sections at Guro Hospital, Seoul, Korea, between May 1993 and December 2001 was performed. Of the 416 women, 283 underwent cesarean sections with FAST and 133 with the traditional technique. RESULTS: Operative time was significantly shorter with FAST (15.3 vs. 42.6 min, P<.05), and FAST was associated with lower blood loss (601 vs. 928 mL, P<.05) and shorter hospital stay (3.7 vs. 6.5 days, P<.05). There were no significant differences in wound infection, voiding difficulty, and postoperative adhesions between the two methods. CONCLUSION: These results suggest that FAST may be the better technique.     

关于心律失常教学课件的设计制作构想

介绍了一种基于计算机动画和视频剪辑技术的心律失常教学课件的设计思想、课件内容及演示特点。     

岗梅根清喉颗粒的制备及临床疗效观察

目的研究岗梅根清喉颗粒的制备方法,并观察其临床疗效。方法与草珊瑚含片对照比较,观察其治疗咽喉炎症的临床疗效,并进行动物急性毒性试验。结果符合颗粒的质量要求。动物试验LD50>1208g生药/kg。治疗组与对照组总有效率均在85%以上,两者对咽喉炎症均具有明显的治疗作用。结论本颗粒组方合理,工艺可行,无毒性;临床疗效满意。     

Vitamin-D Deficiency and Supplementation Altered the Network of the Coronary Arteries in a Rodent Model—In Situ Video Microscopic Technique

The aim of our study was to identify whether vitamin-D deficiency (VDD) can alter the geometry of the coronary-resistance-artery system. Male Wistar rats were divided into vitamin-D-deficient (VD−, n = 10) and vitamin-D-supplemented (VD+, n = 8) groups. After eight weeks, branches and segments of the left-anterior-descending-coronary-artery (LAD) network were analyzed by a video-microscopy technique. Segments were divided into 50 μm-long cylindrical ring units. VDD did not increase the number of morphological abnormalities. The number of segments did not differ between the groups (VD−: 210 and VD+: 224; pooled data of 8 networks). A larger lumen area of branches was found in VD+ group, while 1–4-order branches were lengthier in the VD− group. VD− rats had less rich coronary-resistance-artery networks in terms of 50 µm-long units. (VD−: 6365 vs. VD+: 6602; pooled data of 8 networks). VD+ animals were richer in the 100–350 µm outer diameter range, and VD− animals were richer in the 400–550 µm-diameter units. In VD− rats, 150–200 and 300 µm units were almost missing at higher flow distances from the orifice. Serum vitamin-D alterations caused by dietary changes can affect the geometry of the coronary-artery network, which may contribute to vitamin-D-dependent changes in cardiovascular mortality.     

薯蓣皂素生产工艺的研究进展

介绍薯蓣皂素生产工艺的现状及存在的问题,对生产工艺的研究进展进行总结,并对生产工艺的发展前景提出了观点,认为低污染或无污染的薯蓣皂素生产工艺将成为其发展方向.     

虎杖提取工艺初探

对乙肝康君虎杖进行单方水提、复方水提及７０％醇提工艺比较，结果７０％醇提，总蒽酯提取率为９６．７％，大黄素提取率为９６．１％，但经回收酒精，浓缩，干燥后，总蒽酯保留率为６８．９％，大黄素保留率为６５．６％。     

重庆喀斯特地区金银花的生物学特性及规范化种植技术研究

为促进金银花种植技术在广大喀斯特地区顺利推广,促进中药材产业的顺利发展,并促进喀斯特地区生态顺利恢复,结合喀斯特地区生态恢复技术研究项目的 开展,开展了金银花主要品种忍冬在喀斯特地区的生物学特性研究、生态作用分析和规范化种植技术研究,形成了较为系统金银花规范化种植技术体系,主要包括育苗、移栽、田间管理、病虫害防治及加工...     

细胞共培养技术在医药研究中的应用

细胞共培养由于能更好地模拟体内环境,便于更好的观察细胞与细胞、细胞与培养环境之间的相互作用以及探讨药物的作用机制和可能的作用靶点,填补了单层细胞培养与整体动物实验研究的鸿沟,近年来倍受医药领域的关注,成为药物研发、生物制药领域的研究热点.细胞共培养方法包括直接共培养和间接共培养,主要用于疾病病理基础、新型治疗手段以及药物活性筛选的研究.现有的细胞共培养技术主要用于单一药物的药效学研究,用于联合药物相互作用的研究甚少.中药复方之间协同配伍,减毒增效.细胞共培养技术符合中药多成分、多靶点的作用特点,这对于未来探讨中药联合用药对机体的作用及机制的研究具有一定参考价值,为中药及复方研究提供了一种新的手段.该文就细胞共培养技术的方法与运用进行了概述,并对该技术运用于中药及复方的研究进行了展望.     

经皮椎体成形术治疗15例骨质疏松性椎体压缩性骨折体会

近年来,国内外文献报导经皮椎体成形术治疗骨质疏松性椎体压缩性骨折由于创伤小、效果好、并发症少而大量应用于临床。2009-2010年我们采用该手术治疗骨质疏松性骨折15例,疗效满意,现报告如下。