MicroRNA-125b Accelerates and Promotes PML-RARa-driven Murine Acute Promyelocytic Leukemia

ObjectiveMost acute promyelocytic leukemia cases are characterized by the PML-RARa fusion oncogene and low white cell counts in peripheral blood.MethodsBased on the frequent overexpression of miR-125-family miRNAs in acute promyelocytic leukemia, we examined the consequence of this phenomenon by using an inducible mouse model overexpressing human miR-125b.ResultsMiR-125b expression significantly accelerates PML-RARa-induced leukemogenesis, with the resultant induced leukemia being partially dependent on continued miR-125b overexpression. Interestingly, miR-125b expression led to low peripheral white cell counts to bone marrow blast percentage ratio, confirming the clinical observation in acute promyelocytic leukemia patients.ConclusionThis study suggests that dysregulated miR-125b expression is actively involved in disease progression and pathophysiology of acute promyelocytic leukemia, indicating that targeting miR-125b may represent a new therapeutic option for acute promyelocytic leukemia.     

Structural Response of Steel Jacket-UHPC Retrofitted Reinforced Concrete Columns under Blast Loading

The lateral capacity of exterior concrete columns subjected to a blast load is the key factor in the building collapse probability. Due to potentially severe consequences of the collapse, efforts have been made to improve the blast resistance of existing structures. One of the successful approaches is the use of ultra-high-performance-concrete (UHPC) jacketing for retrofitting a building’s columns. The columns on the first floor of a building normally have higher slenderness due to the higher first story. Since an explosion is more likely to take place at the ground level, retrofitting the columns of the lower floors is crucial to improve a building’s blast resistance. Casting a UHPC tube around a circular RC column can increase the moment of inertia of the column and improve the flexural strength. In this study, a retrofitting system consisting of a UHPC layer enclosed by a thin steel jacket is proposed to improve the blast resistance of buildings in service. Most of the previous research is focused on design aspects of blast-resistant columns and retrofitting systems are mostly based on fiber reinforced polymers or steel jackets. A validated FE model is used to investigate the effectiveness of this method. The results showed significant improvement both at the component and building system levels against combined gravity and blast loading.     

Disseminated intravascular coagulation and decrease in fibrinogen levels induced by vincristine/prednisolone therapy of lymphoid blast crisis of chronic myeloid leukemia

Summary Therapy with vincristine (2 mg i.v. weekly) and prednisolone (100 mg p.o. daily) caused a decrease in fibrinogen levels in nine patients treated for lymphoid blast crisis (LBC) of chronic myeloid leukemia (CML). During the first days of treatment disseminated intravascular coagulation (DIC), evidenced by a positive ethanol gelation test, markedly increased thrombin-antithrombin III complex and fibrin-split product D-dimer levels, and a rapid fall in fibrinogen levels was observed in two patients. The induction of DIC in these two patients caused profuse bleeding in one and necessitated substitution therapy with fibrinogen and platelet concentrates. The remaining seven patients revealed no signs of DIC; nevertheless, four of them showed a moderate increase in D-dimer levels after initiation of therapy. In these patients a well-known side effect of long-term steroid therapy, namely a decrease of fibrinogen levels, was observed within the first week of treatment. Fibrinogen levels did not fall below 150 mg/dl and increased after dose reduction from 100 mg/day to 50 mg/day. We conclude from our results that two types of disturbances in fibrinogen metabolism can be observed during vincristine/prednisolone therapy of LBC of CML: (a) a decrease of fibrinogen levels due to a steroid-mediated impairement of liver synthesis, and (b) a rapid fall in fibrinogen levels in the course of DIC, most likely induced by the release of procoagulants from detoriorating blast cells, leading to severe bleeding in selected cases.     

Integrating a Top-Gas Recycling and CO2 Electrolysis Process for H2-Rich Gas Injection and Reduce CO2 Emissions from an Ironmaking Blast Furnace

Introducing CO₂ electrochemical conversion technology to the iron-making blast furnace not only reduces CO₂ emissions, but also produces H₂ as a byproduct that can be used as an auxiliary reductant to further decrease carbon consumption and emissions. With adequate H₂ supply to the blast furnace, the injection of H₂ is limited because of the disadvantageous thermodynamic characteristics of the H₂ reduction reaction in the blast furnace. This paper presents thermodynamic analysis of H₂ behaviour at different stages with the thermal requirement consideration of an iron-making blast furnace. The effect of injecting CO₂ lean top gas and CO₂ conversion products H₂–CO gas through the raceway and/or shaft tuyeres are investigated under different operating conditions. H₂ utilisation efficiency and corresponding injection volume are studied by considering different reduction stages. The relationship between H₂ injection and coke rate is established. Injecting 7.9–10.9 m³/tHM of H₂ saved 1 kg/tHM coke rate, depending on injection position. Compared with the traditional blast furnace, injecting 80 m³/tHM of H₂ with a medium oxygen enrichment rate (9%) and integrating CO₂ capture and conversion reduces CO₂ emissions from 534 to 278 m³/tHM. However, increasing the hydrogen injection amount causes this iron-making process to consume more energy than a traditional blast furnace does.     

姜黄素对胸部爆震致小鼠肺组织氧化应激损伤的影响

目的 探讨姜黄素对胸部爆震致小鼠肺组织氧化应激损伤的影响.方法 将30只C57BL/6小鼠随机分为对照组、模型组、模型+姜黄素组,伤后24 h处死小鼠,左肺进行10％甲醛固定,石蜡包埋切片,右肺-80℃保存.HE染色观察肺组织爆震后病理改变;活性氧生成(reactive oxygen species,ROS)检测肺组织...     

Comparison of Testing Methods for Evaluating the Resistance of Alkali-Activated Blast Furnace Slag Systems to Sulfur Dioxide

Alkali-activated systems (AAS) represent an ecologically and economically sustainable inorganic binder as an alternative to ordinary Portland cement (OPC). One of the main benefits of AAS is their durability in aggressive environments, which can be equal or even better than that of OPC. In this paper, the influence of the type of alkaline activator in alkali-activated blast furnace slag (AAS) in terms of resistance to sulfur dioxide corrosion was investigated. The durability testing process was based on the CSN EN ISO 3231 standard and simultaneously compared with mortar samples prepared by using Blastfurnace cement CEM III/A 32.5R. The degradation progress was evaluated by employing several different methods such as observing the compressive strength development, weight change evaluation, non-destructive testing methods like ultrasound or impact echo technique, or visual phenolphthalein technique. Subsequently, fundamental characterization of samples by the XRD method was performed during the degradation test. The obtained results indicate that none of the testing methods used could be prioritized over others to determine the resistance of AAS against the action of sulfur dioxide. For this reason, the durability testing of AAS remains an issue, and the development of specific standards considering the behavior of AAS seems necessary.     

眼眶爆裂骨折的CT表现

【目的】探讨CT检查对眼眶爆裂骨折的早期诊断价值。【方法】回顾性分析了47倒眼眶爆裂骨折的临床及CT表现及好发部位。【结果】眼眶骨折,直接征象是眶壁骨质连续性中断,粉碎,移位及曲度失常。根据骨折的特点可将眼眶骨折分为三类,即单眼眶骨折,多眼眶骨折,并发其他面部直接骨折的眼眶骨折。眼眶爆裂骨折最好发于眼眶内侧壁和底壁,占93．6％。副鼻窦内“泪滴”征是诊断眼眶下壁骨折的特异性间接征象。【结论】CT对早期诊断眼眶爆裂骨折有重要的临床价值。     

脊髓爆震伤后早期脊髓前角运动神经元的形态学变化

目的:建立脊髓爆震伤动物实验模型,探讨脊髓爆震伤后早期脊髓前角运动神经元形态学变化. 方法:将36只家兔随机分为对照组(A组,n=12) ,6 h实验组(B组,n=12)及24 h实验组(C组,n=12),采用0.9 g单质金属炸药黑索金(RDX)将B,C组家兔炸伤,分别于伤后6 h及24 h两个时间点取材,进行HE染色、甲苯胺蓝染色及银浸染色,观察脊髓神经元形态学改变. 结果:脊髓爆震伤后6 h脊髓运动神经元发生可逆性改变,部分神经元坏死,死亡均数为12.58±2.23,而伤后24 h脊髓运动神经元大量坏死,死亡均数达到了31.52±2.69,与对照组相比差异有统计学意义(P<0.01). 结论:脊髓爆震伤后6 h内脊髓运动神经元以可逆性改变为主,提出早期发现和治疗的重要性.