人血管抑素A K(1-3)基因的克隆表达、纯化及活性鉴定

目的 克隆人Angiostatin K(1-3)基因,获得有活性的重组人血管抑素蛋白,为进一步开发应用奠定基础.方法 以人新鲜肝脏组织为材料,通过RT-PCR得到人Angiustatin基因的AK(1-3)片段.构建重组质粒pET30a-Angiostatin,转化表达菌Rosetta(DE3),对转化子进行诱导表达,利用Ni-NTA亲和层析纯化目的 产物,并验证其活性.结果 获得了人Angiostatin基因AK(1-3)片段的正确序列,表达和纯化了人血管抑素蛋白,表达量占菌体总蛋白的30%,纯化后证明表达产物具有较高纯度(达到90%).结论 Anginstatin K(1-3)可在原核融合蛋白表达载体中表达,且得到有活性的目的 蛋白.     

Blocking-ELISA for detection of specific antibodies to the glycoproteins of the human immunodeficiency virus type 1 (HIV-1)

A blocking ELISA was developed to confirm the specificity of screening tests for anti-HIV-1 antibodies. A murine monoclonal antibody (McAb) raised against recombinant gp160 was used in combination with a commercial technique (ELA-VIA-1). After determining the optimal experimental conditions, the assay was applied to 92 samples presenting different reactivities by Western blot (WB) analysis. All the sera containing antibodies to gp160/gp120 (53) were positive in our assay. The six patients who sero converted showed a low positivity by ELAVIA-1 (optical density near the cutoff value) reacted by blocking-ELAVIA-1 with an McAb binding inhibition greater than 85%. By contrast, negative samples (29) and specimens that exhibited reactivity only against gag-proteins (10) were not detected (McAb binding inhibition smaller than 15%). This sensitive and specific blocking-ELAVIA-1 represents a convenient alternative to WB as a confirmatory test. The technique is time-saving and inexpensive and can easily be integrated with a screening test for diagnostic or epidemiologic studies on HIV-1 infection.     

A Cross-Sectional,Correlational Study Comparing Individual Characteristics of Younger and Older Nursing Home Residents using Western Canadian Resident Assessment Instrument–Minimum Data Set (RAI-MDS) 2.0

ObjectiveTo compare characteristics of nursing home (NH) residents by age categories in Western Canada.DesignA cross-sectional, correlational analysis of secondary data.Setting and Participants89,231 residents living in Western Canada NHs in the provinces of Alberta, Manitoba, and British Columbia in 2016 and 2017.MethodsResident characteristics (age, sex, marital status, body mass index, medical diagnoses, cognitive function, physical function, depressive symptoms) came from the Resident Assessment Instrument–Minimum Data Set 2.0 and were analyzed using chi-square, analysis of variance, and post hoc pairwise tests. Human developmental stage age categories were used to create 5 age groups: 18-34, 35-50, 51-64, 65-80, and 81 years and older.ResultsThe demographics, medical diagnoses, cognitive function, and physical function characteristics of NH residents among 5 age groups differed considerably (all P < .001). Residents aged 18-34 years were predominately male, never married, with a higher incidence of paralysis and traumatic brain injury. Residents aged 35-50 years had a higher incidence of stroke and multiple sclerosis, and residents aged 51-64 years mainly were morbidly obese and more prone to depression. Residents aged 65-80 years were predominately married and more prone to diabetes, and residents aged 81 years and older were predominately widowed, with a higher incidence of dementia compared with others.Conclusions and ImplicationsFindings describe the uniqueness of younger NH age groups and indicate that the youngest NH residents often have the severe disability and a modest support system (as defined by partnered status) compared to older residents in NHs. Future studies must analyze longitudinal data that track the growth of, and changes in, residents’ health and functional status.     

中西医结合治疗眼底病的实验和临床研究

眼底病是一种复杂的致盲率很高的眼部疾病 ,包括视网膜脱离、视网膜玻璃体出血和增殖性玻璃体视网膜病变 (PVR)等疾病。本文采用中西医结合的治疗方法对动物模型和 1998～ 2 0 0 0年此类眼病患者进行研究 ,疗效显著 ,使眼底病的治愈率有了很大幅度的提高。中医药对眼底病的治疗常用活血化瘀复方治疗 ,对于从动物实验及临床两方面同时进行中西医结合的系统治疗研究 ,尚未见有报道。     

中西医结合治疗老年慢性肾功能衰竭

目的观察中西医结合治疗老年慢性肾功能衰竭的临床疗效.方法老年慢性肾功能衰竭病人57例,随机分为治疗组和对照组.对照组用常规西药治疗,治疗组在上述治疗基础上加用降压排毒饮和中药灌肠,观察治疗前后血压、尿素氮、血肌酐、内生肌酐清除率及血脂变化.结果治疗组经治疗后血压、尿素氮、血肌酐、胆固醇、甘油三酯显著下降,而内生肌酐清除率、高密度脂蛋白上升;对照组除血压略下降,其他无显著变化,两组比较,有显著差异,P＜0.01.结论中西医结合疗法在降压、降脂、改善肾功能方面,均显著优于对照组.     

缺氧预适应小鼠脑组织中缺氧诱导因子-1的表达

为探讨缺氧诱导因子 1 (HIF 1 )在缺氧预适应小鼠脑组织中的表达 ,用Western印迹法检测慢性缺氧 (H)和不缺氧 (C)HeLa细胞、急性重复性缺氧 0次 (H0 )、1次 (H1)、4次 (H4 )小鼠脑组织中的HIF 1α。结果 :慢性缺氧HeLa细胞中可检测到较多的HIF 1α,H0 组脑组织中检测不到HIF 1α ,H1组检测到微量HIF 1α,H4 组检测到较多HIF 1α。结果显示 :脑组织中HIF 1α的含量随缺氧预适应的产生而逐步增加 ,提示HIF 1可能参与缺氧预适应的形成。     

《中西医结合防治小儿腹泻病专家共识》解读

小儿腹泻病是儿童消化系统最常见的疾病,2022年中国中西医结合学会儿科专业委员会制定了《中西医结合防治小儿腹泻病专家共识》。现结合临床实践及最新的研究结果,对《中西医结合防治小儿腹泻病专家共识》中小儿腹泻病的发病机制、诊断和治疗中的重点内容进行解读,以期加深医务工作者对《中西医结合防治小儿腹泻病专家共识》的认识,促进中西医结合规范化治疗小儿腹泻病。     

数据挖掘在名老中医经验传承的应用现状与智能化趋势

现在,数据挖掘技术正朝着智能化的趋势快速发展,人工智能（AI）在西医学的广泛应用,尤其是AI分析方法在疾病早期诊断与评估、疾病的治疗策略与医疗资源管理这几个方面突出的使用效果,为AI与中医药的结合提供了有价值的参考,尤其在名老中医经验的学习与传承方面。近十余年来,名老中医经验学习与传承领域数据挖掘技术的研究成果颇多,在梳理常用数据挖掘工具与分析方法的基础上,结合AI分析方法在西医学的应用,可以对中医药领域数据挖掘的智能化学习发展方向进行预测与展望,为继承与发展名老中医经验提供更高效的途径,加快中医现代化研究。     

肌肉骨骼慢性疼痛诊治专家共识

肌肉骨骼系统慢性疼痛是临床最常见的慢性疼痛。随着人口老龄化,慢性肌肉骨骼疼痛的总体负担将继续加重,对安全有效的疼痛管理的需求也将日益增加。由于文化、经济和监管因素不同,国际指南可能不完全适合中国病人。可用的国内指南大多只针对于药物治疗,且没有阐述中医药在肌肉骨骼系统慢性疼痛诊治中的重要作用。为了制定慢性肌肉骨骼疼痛的中西医结合专家共识,我们召开会议回顾并讨论近期循证医学证据、临床经验和我们目前面临的实践挑战,为慢性肌肉骨骼疼痛的全面评估和管理提供了建议。这些共识建议不是指令性的,而是作为临床实践决策的指南,慢性肌肉骨骼疼痛的最佳治疗方案应该是个性化的。